Recombinant Mouse EGFLAM Flag-tag Protein, CF Summary
Accession # Q4VBE4.1
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Supplied as a 0.2 μm filtered solution in PBS.
|Shipping||The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
When Recombinant Human Dystroglycan Fc Chimera (Catalog # 10223-DG) is immobilized at 1 µg/mL (100 µL/well), the concentration of Recombinant Mouse EGFLAM Flag-tag Protein (Catalog # 10367-EG) binds with an ED50 of 0.075-0.45 µg/mL.
2 μg/lane of Recombinant Mouse EGFLAM (Catalog # 10367-EG) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 103-198 kDa.
EGFLAM (EGF-like, fibronectin type-III and laminin G-like domain-containing protein), also known as Pikachurin or Nectican, is a highly conserved extracellular matrix-like protein with a molecular weight around 110 kDa. Murine EGFLAM has 1,017 amino acids (∼110 kDa) with a 24 amino acids signal peptide. It can bind to alpha -dystroglycan and has been found to localize mainly in the synaptic cleft of photoreceptor ribbon synapses (1). At the ribbon synapses, EGFLAM protein is colocalized with both dystrophin and dystroglycan, where it plays critical roles in interactions between the photoreceptor ribbon synapse and bipolar dendrites (1, 2). EGFLAM gene is also expressed in other organs and tissues including brain, endocrine tissues and muscle tissues (3). EGFLAM has been considered as a new biomarker for theca interna and granulosa cells (4), a novel susceptibility locus for aortic aneurysm (3), a hypomethylated biomarkers for ovarian cancer (5), and a prognostic biomarker and therapeutic target for glioblastoma (6). EGFLAM contains 11 intracellular disulfide bonds and one O-glycosylation site that attaches to chondroitin sulfate and heparan sulfate. This protein is composed of two fibronectin type-III domains followed by three EGF-like domain and three Laminin G-like domains. Mouse EGFLAM shares 89% and 95% amino acid identity to human and rat EGFLAM, respectively.
- Sato, S. et al. (2008) Nat. Neurosci. 11:923.
- Han, J. and Townes-Anderson, E. (2012) PloS One 7:e50552.
- Yamada, Y. et al. (2017) Int. J. Mol. Med. 39:1091.
- Hatzirodos, N. et al. (2015) PLoS One 10:e0119800.
- Gu, X.H. et al. (2009) Zhonghua Fu Chan Ke Za Zhi 44:754.
- Chen, Juhui, et al. (2019) Cancer Biomarkers 24:343.
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