Recombinant Mouse Ephrin-B3 Fc Chimera Protein, CF

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7655-EB-050
R&D Systems Recombinant Proteins and Enzymes
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Citations (4)
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Recombinant Mouse Ephrin-B3 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Mouse Ephrin-B3 Fc Chimera is coated at 2 μg/mL, Recombinant Mouse EphB3 Fc Chimera (Catalog # 432-B3) binds with an apparent KD <1 nM.
Source
Mouse myeloma cell line, NS0-derived mouse Ephrin-B3 protein
Mouse Ephrin-B3
(Leu28-Ala227)
Accession # O35393
IEGRMDP Mouse IgG2A
(Glu98-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Leu28
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
49.2 kDa (monomer)
SDS-PAGE
58-61 kDa, reducing conditions

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7655-EB

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7655-EB

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: Ephrin-B3

Ephrin-B3, also known as Elk-L3, LERK8, Eplg8, NLERK-2, and EFL6, is an approximately 50 kDa member of the Ephrin-B family of transmembrane ligands that bind and induce the tyrosine autophosphorylation of Eph receptors. The extracellular domains (ECD) of Ephrin-B ligands are structurally related to GPI-anchored Ephrin-A ligands. Eph‑Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression. Ephrin-B3 preferentially interacts with receptors in the EphB family and also with EphA4. The binding of Ephrin-B3 to Eph proteins also triggers reverse signaling through Ephrin-B3 (1, 2). Mature mouse Ephrin-B3 consists of a 200 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 92 aa cytoplasmic domain (3, 4). Within the ECD, mouse Ephrin-B3 shares 96% and 98% aa sequence identity with human and rat Ephrin-B3, respectively. Ephrin-B3 is expressed on oligodendrocytes and neurons in the hippocampus and along the midline of the spinal cord (5-9). It is up-regulated in glioma and promotes tumor cell invasion and migration (10). Ephrin-B3 functions as a repulsive axon guidance molecule by inducing growth cone collapse, neurite retraction, and axon pruning (5-8). Its repulsive effect along the spinal cord midline restricts motor neuron axons to their ipsilateral sides, thereby maintaining the independence of voluntary left side/right side movements (8, 9). Ephrin-B3 plays a role in the regulation of excitatory synapse density and synaptic maturation (6, 11, 12). It also functions as a cellular receptor for Nipah virus (13) and can induce the migration of memory B cells (14).

References
  1. Miao, H. and B. Wang (2009) Int. J. Biochem. Cell Biol. 41:762.
  2. Pasquale, E.B. (2010) Nat. Rev. Cancer 10:165.
  3. Tang, X.X. et al. (1997) Genomics 41:17.
  4. Bergemann, A.D. et al. (1998) Oncogene 16:471.
  5. Benson, M.D. et al. (2005) Proc. Natl. Acad. Sci. USA 102:10694.
  6. Xu, N.-J. et al. (2011) Nat. Neurosci. 14:1421.
  7. Xu, N.-J. and M. Henkemeyer (2009) Nat. Neurosci. 12:268.
  8. Kullander, K. et al. (2001) Genes Dev. 15:877.
  9. Yokoyama, N. et al. (2001) Neuron 29:85.
  10. Nakada, M. et al. (2006) Cancer Res. 66:8492.
  11. McClelland, A.C. et al. (2010) Proc. Natl. Acad. Sci. USA 107:8830.
  12. Antion, M.D. et al. (2010) Mol. Cell. Neurosci. 45:378.
  13. Negrete, O.A. et al. (2006) PLoS Pathog. 2:e7.
  14. Holen, H.L. et al. (2011) Scand. J. Immunol. 74:144.
Entrez Gene IDs
1949 (Human); 13643 (Mouse)
Alternate Names
EFL-6; Efnb3; Elk-L3; EPH-related receptor tyrosine kinase ligand 8; Ephrin B3; EphrinB3; Ephrin-B3; Epl8; EPLG8EPH-related receptor transmembrane ligand ELK-L3; LERK-8; LERK8EFL6; NLERK-2

Citations for Recombinant Mouse Ephrin-B3 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Detection of Nipah and Hendra Viruses Using Recombinant Human Ephrin B2 Capture Virus in Immunoassays
    Authors: M Yang, W Zhu, T Truong, B Pickering, S Babiuk, D Kobasa, L Banadyga
    Viruses, 2022;14(8):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: ELISA Capture
  2. Identification and characterization of Nanobodies targeting the EphA4 receptor
    Authors: L Schoonaert, L Rué, B Roucourt, M Timmers, S Little, L Chávez-Gut, M Dewilde, P Joyce, A Curnock, P Weber, J Haustraete, G Hassanzade, B De Stroope, L Van Den Bo, P Van Damme, R Lemmens, W Robberecht
    J. Biol. Chem., 2017;292(27):11452-11465.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Nipah virus attachment glycoprotein stalk C-terminal region links receptor binding to fusion triggering.
    Authors: Liu Q, Bradel-Tretheway B, Monreal A, Saludes J, Lu X, Nicola A, Aguilar H
    J Virol, 2015;89(3):1838-50.
    Species: Virus
    Sample Types: Whole Cells
    Applications: Bioassay
  4. EphB4 forward signalling regulates lymphatic valve development.
    Authors: Zhang, Gu, Brady, John, Liang, Wei-Chin, Wu, Yan, Henkemeyer, Mark, Yan, Minhong
    Nat Commun, 2015;6(0):6625.
    Species: Mouse
    Sample Types: Antibody
    Applications: Enzyme Assay

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