Recombinant Mouse Fc alpha/mu R His-tag Protein, CF Summary
Trp47-Arg455, with a C-terminal 6-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 500 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
When Recombinant Mouse Fc alpha/mu R (Catalog # 10069-FC) is coated at 2ug/mL, 100 uL/well, Mouse IgM binds with an ED50 of 0.04-0.24 μg/mL.
Background: Fc alpha/mu R
Fc alpha/mu Receptor (FCAMR), designated CD351, is an approximately 60-kDa transmembrane protein that serves as a receptor for IgA and IgM immunoglobulins (1). Mature mouse FCAMR consists of a 420 amino acid (aa) extracellular domain with one Ig-like V-type domain, a 21 aa transmembrane segment, and a 59 aa cytoplasmic domain. Within the ECD, mouse FCAMR shares 51% aa and 80% aa sequence identity with human and rat FCAMR, respectively. FCAMR is expressed on B cells, macrophages, and kidney mesangial cells (2-4). It binds to both IgA and IgM in immune complexes but not to monomeric immunoglobulin (2, 5). FCAMR participates in pathogen clearance as well as foam cell formation by mediating the internalization of IgM-opsonized microbes and oxidized LDL-containing particles (2, 6).
- Wang, H. et al. (2016) Front. Immunol. 7:99.
- Shibuya, A. et al. (2000) Nat. Immunol. 1:441.
- Matesanz-Isabel, J. et al. (2011) Immunol. Lett. 134:104.
- McDonald, K.J. et al. (2002) Biochem. Biophys. Res. Commun. 290:438.
- Ghumra, A. et al. (2009) Eur. J. Immunol. 39:1147.
- Feng, X. et al. (2010) Atherosclerosis 208:396.
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