Recombinant Mouse Fc alpha/mu R His-tag Protein, CF

Catalog # Availability Size / Price Qty
10069-FC-050
Recombinant Mouse Fc alpha/mu R His-tag Protein, CF Binding Activity
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Recombinant Mouse Fc alpha/mu R His-tag Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Mouse Fc alpha /μ R is immobilized at 2 μg/mL, 100 μL/well, the concentration of Mouse IgM that produces 50% of the optimal binding response is 0.04-0.24 μg/mL.
Source
Mouse myeloma cell line, NS0-derived mouse Fc alpha/mu R protein
Trp47-Arg455, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Trp47
Predicted Molecular Mass
44 kDa
SDS-PAGE
97-112 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10069-FC

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Data Image

Binding Activity Binding Activity View Larger

When Recombinant Mouse Fc alpha/mu R (Catalog # 10069-FC) is coated at 2ug/mL, 100 uL/well, Mouse IgM binds with an ED50 of 0.04-0.24 μg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Fc alpha/mu R

Fc alpha/mu Receptor (FCAMR), designated CD351, is an approximately 60-kDa transmembrane protein that serves as a receptor for IgA and IgM immunoglobulins (1). Mature mouse FCAMR consists of a 420 amino acid (aa) extracellular domain with one Ig-like V-type domain, a 21 aa transmembrane segment, and a 59 aa cytoplasmic domain. Within the ECD, mouse FCAMR shares 51% aa and 80% aa sequence identity with human and rat FCAMR, respectively. FCAMR is expressed on B cells, macrophages, and kidney mesangial cells (2-4). It binds to both IgA and IgM in immune complexes but not to monomeric immunoglobulin (2, 5). FCAMR participates in pathogen clearance as well as foam cell formation by mediating the internalization of IgM-opsonized microbes and oxidized LDL-containing particles (2, 6).

References
  1. Wang, H. et al. (2016) Front. Immunol. 7:99.
  2. Shibuya, A. et al. (2000) Nat. Immunol. 1:441.
  3. Matesanz-Isabel, J. et al. (2011) Immunol. Lett. 134:104.
  4. McDonald, K.J. et al. (2002) Biochem. Biophys. Res. Commun. 290:438.
  5. Ghumra, A. et al. (2009) Eur. J. Immunol. 39:1147.
  6. Feng, X. et al. (2010) Atherosclerosis 208:396.
Long Name
Fc alpha/mu Receptor
Entrez Gene IDs
83953 (Human); 64435 (Mouse); 681070 (Rat)
Alternate Names
CD351 antigen; CD351; Fc alpha/mu R; Fc alpha/mu receptor; Fc receptor, IgA, IgM, high affinity; FCA/MR; FCAMR; FKSG87; high affinity immunoglobulin alpha and immunoglobulin mu Fc receptor; immunity related factor; receptor for Fc fragment of IgA and IgM; uR

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