Recombinant Mouse FcgR4/CD16-2 Protein, CF

Catalog # Availability Size / Price Qty
1974-CD-050
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Mouse FcgR4/CD16-2 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to bind mouse IgG with an estimated Kd <1 nM.
Source
Mouse myeloma cell line, NS0-derived mouse FcgR4/CD16-2 protein
Gly21-Gln203, with a C-terminal 10-His tag
Accession #
N-terminal Sequence
Analysis
Gly21
Predicted Molecular Mass
22.3 kDa
SDS-PAGE
25-35 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1974-CD

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: FcgR4/CD16-2

Receptors for the Fc region of IgG (Fc gamma Rs) are members of the Ig superfamily that function in the activation or inhibition of immune responses such as degranulation, phagocytosis, ADCC (antibody-dependent cellular toxicity), cytokine release, and B cell proliferation (1-3). The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma  RI (also known as CD64), Fc gamma  RII (CD32), and Fc gamma  RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. The CD64 proteins are high affinity receptors
(~10-8-10-9 M) capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities (~10-6-10-7 M) only recognizing IgG aggregates surrounding multivalent antigens (1, 4). Fc gamma Rs that deliver an activating signal either have an intrinsic immunoreceptor tyrosine-based activation motif (ITAM) within their cytoplasmic domains or associate with one of the ITAM-bearing adapter subunits, Fc R gamma or zeta (3, 5). The only inhibitory member in human and mouse, Fc gamma  RIIb, has an intrinsic cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM). The coordinated functioning of activating and inhibitory receptors is necessary for successful initiation, amplification, and termination of immune responses (5).

Human CD16 is encoded by two genes; Fc gamma RIIIA is an activating transmembrane receptor expressed in macrophages and NK cells, and Fc gamma RIIIB is a GPI-linked protein on granulocytes (2). Mouse Fc gamma RIII and human Fc gamma RIIIA share 44% amino acid identity and are frequently described as functional equivalents (5).

CD16-2, also referred to as Fcg R4, is a protein that is more closely related to human Fc gamma  RIIIA (60% amino acid identity) and may be its true ortholog (6). Similar to other activating receptors, it possesses a charged residue within its transmembrane domain that is necessary for association with an ITAM bearing accessory protein. CD16-2 RNA expression was observed in peripheral blood leukocytes, spleen, thymus, colon, and intestine.

References
  1. van de Winkel, J. and P. Capes (1993) Immunol. Today 14:215. 
  2. Raghaven, M. and P. Bjorkman (1996) Annu. Rev. Cell Dev. Biol. 12:181.  
  3. Ravetch, J. and S. Bolland (2001) Annu. Rev. Immunol. 19:275. 
  4. Takai, T. (2002) Nature Rev. Immunol. 2:580.
  5. Ravetch, J. and L. Lanier (2000) Science 290:84.
  6. Mechetina, L. et al. (2002) Immunogenetics 54:463.
Long Name
Fc receptor, IgG, low affinity IV
Entrez Gene IDs
246256 (Mouse)
Alternate Names
CD16-2; FcgR4; Fcrl3

Citation for Recombinant Mouse FcgR4/CD16-2 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. IgEb immune complexes activate macrophages through FcgammaRIV binding.
    Authors: Hirano M, Davis RS, Fine WD, Nakamura S, Shimizu K, Yagi H, Kato K, Stephan RP, Cooper MD
    Nat. Immunol., 2007;8(7):762-71.
    Species: Human
    Sample Types: Antibody
    Applications: Surface Plasmon Resonance

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