Recombinant Mouse FGFR1 Fc Chimera Protein, CF

R&D Systems | Catalog # 10135-FR

R&D Systems
100% Guarantee

Key Product Details

  • R&D Systems NS0-derived Recombinant Mouse FGFR1 Fc Chimera Protein (10135-FR)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

P16092-6

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
View all FGFR1 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse FGFR1 protein
Mouse FGF R1
(Arg22-Ile376)
Accession # P16092-6
 IEGRMDP Mouse IgG2A
(Glu98-Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Arg22

Predicted Molecular Mass

66 kDa

SDS-PAGE

95-115 kDa, under reducing conditions

Activity

Measured by its ability to inhibit FGF acidic-dependent proliferation of NR6R‑3T3 mouse fibroblast cells.
The ED50 for this effect is 15-90 ng/mL

Scientific Data Images for Recombinant Mouse FGFR1 Fc Chimera Protein, CF

Recombinant Mouse FGFR1 Fc Chimera Protein Inhibition Activity

Recombinant Mouse FGFR1 Fc Chimera Protein Inhibition Activity

Recombinant Mouse FGF R1 Fc Chimera (Catalog # 10135-FR) inhibits Recombinant Mouse FGF acidic (Catalog # 4686-FA) induced proliferation of NR6R-3T3 mouse fibroblast cells. The ED50 for this effect is 15-90 ng/mL.

Recombinant Mouse FGFR1 Fc Chimera Protein SDS-PAGE

Recombinant Mouse FGFR1 Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Mouse FGF R1 Fc Chimer (Catalog # 10135-FR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® blue staining, showing bands at 95-115 kDa and 190-230 kDa, respectively.

Formulation, Preparation, and Storage

10135-FR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: FGFR1

Fibroblast growth factor receptor 1 (FGF R1) is a member of a tyrosine kinase family that acts as cell-surface receptors for fibroblast growth factors (FGFs). The FGF R family consists of four members that differ in their ligand affinities and play essential roles in the regulation of embryonic development, cell proliferation, differentiation, and migration (1, 2). Mature mouse FGF R1 consists of a 355 amino acid (aa) extracellular domain (ECD) composed of three immunoglobulin-like domains, a single transmembrane domain and a cytoplasmic tyrosine kinase domain (1). The ECD of mouse FGF R1 shares 98% and 99% aa identity with the human and rat ECD, respectively. FGF R1 binds both acidic and basic fibroblast growth factors and is involved in limb induction (2, 3). Alternative splice variants of this gene have been reported. This product is the longest variant, isoform 1 (1, 4). FGF Rs play essential roles in the regulation of embryonic development, cell proliferation, differentiation and migration (5). Upon FGF and heparan sulfate binding, the FGF Rs form homodimers and the kinase domains are activated (6, 7). Alterations in the binding regulates FGF bioactivity (8).

References

  1. Beer, H.-D. et al. (2000) J. Biol. Chem. 275:16091.
  2. Ornitz, D.M. et al. (1992) Mol. Cell. Bio. 12:240.
  3. Cuiling, Li. et al. (2005) Dev. 132:4755.
  4. Kouhara, H. et al. (1991) Biochem. Biophys. Res. Commun. 176:31.
  5. Turner, N and Grose, R. (2010) Nat. Rev. Cancer 10:116.
  6. Pantoliano, M.W. et al. (1994) Biochemistry 33:10229.
  7. Ornitz, D.M. et al. (1996) J. Biol. Chem. 271:15292.
  8. Guerrini, M. et al. (2007) Curr. Pharm. Des. 13:2045.

Long Name

Fibroblast Growth Factor Receptor 1

Alternate Names

CD331, FGF R1, Flt-2

Entrez Gene IDs

2260 (Human); 14182 (Mouse)

Gene Symbol

FGFR1

UniProt

P16092-6

Additional FGFR1 Products

Product Documents for Recombinant Mouse FGFR1 Fc Chimera Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Mouse FGFR1 Fc Chimera Protein, CF

For research use only

Customer Reviews for Recombinant Mouse FGFR1 Fc Chimera Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Mouse FGFR1 Fc Chimera Protein, CF and earn rewards!

Have you used Recombinant Mouse FGFR1 Fc Chimera Protein, CF?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Proteins and Enzymes