Recombinant Mouse FGFR1 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
10135-FR-050
Recombinant Mouse FGFR1 Fc Chimera Protein, CF Inhibition Activity
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Recombinant Mouse FGFR1 Fc Chimera Protein, CF Summary

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit FGF acidic-dependent proliferation of NR6R‑3T3 mouse fibroblast cells. The ED50 for this effect is 15-90 ng/mL
Source
Mouse myeloma cell line, NS0-derived mouse FGF R1 protein
Mouse FGF R1
(Arg22-Ile376)
Accession # P16092-6
IEGRMDP Mouse IgG2A
(Glu98-Lys330)
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Arg22
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
66 kDa
SDS-PAGE
95-115 kDa, under reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10135-FR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Inhibition Activity Inhibition Activity View Larger

Recombinant Mouse FGF R1 Fc Chimera (Catalog # 10135-FR)inhibits Recombinant Mouse FGF acidic (Catalog # 4686-FA) induced proliferationof NR6R‑3T3mouse fibroblast cells. The ED50 for this effect is 15‑90 ng/mL.

SDS-PAGE SDS-PAGE View Larger

2 μg/lane of Recombinant Mouse FGF R1 Fc Chimer (Catalog #10135-FR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR)conditions and visualized by Coomassie® blue staining, showing bands at 95-115 kDa and  190-230 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FGFR1

Fibroblast growth factor receptor 1 (FGF R1) is a member of a tyrosine kinase family that acts as cell-surface receptors for fibroblast growth factors (FGFs). The FGF R family consists of four members that differ in their ligand affinities and play essential roles in the regulation of embryonic development, cell proliferation, differentiation, and migration (1, 2). Mature mouse FGF R1 consists of a 355 amino acid (aa) extracellular domain (ECD) composed of three immunoglobulin-like domains, a single transmembrane domain and a cytoplasmic tyrosine kinase domain (1). The ECD of mouse FGF R1 shares 98% and 99% aa identity with the human and rat ECD, respectively. FGF R1 binds both acidic and basic fibroblast growth factors and is involved in limb induction (2, 3). Alternative splice variants of this gene have been reported. This product is the longest variant, isoform 1 (1, 4). FGF Rs play essential roles in the regulation of embryonic development, cell proliferation, differentiation and migration (5). Upon FGF and heparan sulfate binding, the FGF Rs form homodimers and the kinase domains are activated (6, 7). Alterations in the binding regulates FGF bioactivity (8).

References
  1. Beer, H.-D. et al. (2000) J. Biol. Chem. 275:16091.
  2. Ornitz, D.M. et al. (1992) Mol. Cell. Bio. 12:240.
  3. Cuiling, Li. et al. (2005) Dev. 132:4755.
  4. Kouhara, H. et al. (1991) Biochem. Biophys. Res. Commun. 176:31.
  5. Turner, N and Grose, R. (2010) Nat. Rev. Cancer 10:116.
  6. Pantoliano, M.W. et al. (1994) Biochemistry 33:10229.
  7. Ornitz, D.M. et al. (1996) J. Biol. Chem. 271:15292.
  8. Guerrini, M. et al. (2007) Curr. Pharm. Des. 13:2045.
Long Name
Fibroblast Growth Factor Receptor 1
Entrez Gene IDs
2260 (Human); 14182 (Mouse)
Alternate Names
basic fibroblast growth factor receptor 1; BFGFR; bFGF-R-1; CD331 antigen; CD331; CEK; EC 2.7.10; EC 2.7.10.1; FGF R1; FGFBR; FGFR1; FGFR-1; fibroblast growth factor receptor 1; FLGH3; FLJ99988; Flt-2; FLT2H4; Fms-like tyrosine kinase 2; fms-related tyrosine kinase 2; H2; H5; HBGFR; heparin-binding growth factor receptor; hydroxyaryl-protein kinase; KAL 2; KAL2; N-SAM; OGD; Proto-oncogene c-Fgr; soluble FGFR1 variant 1; soluble FGFR1 variant 2

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