Recombinant Mouse Fibulin 7 Protein, CF

R&D Systems | Catalog # 8606-FB

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Key Product Details

  • R&D Systems CHO-derived Recombinant Mouse Fibulin 7 Protein (8606-FB)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

Q501P1

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
View all Fibulin 7 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived mouse Fibulin 7 protein
Gln25-Phe440, with an N-terminal HA Tag

Purity

>90%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Tyr 

Predicted Molecular Mass

47 kDa

SDS-PAGE

53-63 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of SVEC4‑10 mouse vascular endothelial cells.
The ED50 for this effect is 0.7-3.5 μg/mL.

Formulation, Preparation, and Storage

8606-FB
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Fibulin 7

Fibulin 7, also known as TM14, is an approximately 46 kDa member of the fibulin family of proteins (1, 2). Structurally, Fibulin 7 contains an N-terminal Sushi domain, 3 central tandem EGF modules, and a C-terminal domain homologous to the fibulin module (2). Mouse Fibulin 7 mRNA is highly expressed in teeth, with weaker expression in brain, kidneys, muscles, and bones (2). Mouse Fibulin 7 shares 86% amino acid sequence identity with human Fibulin 7. Fibulin 7 interacts with several extracellular molecules, including Fibronectin, DSPP, and Fibulin 1 (2). A C-terminal fragment of Fibulin 7 was able to bind Human Umbilical Vein Endothelial Cells (HUVECs) and inhibit HUVEC tube formation and vessel sprouting in aortic ring assays, suggesting that Fibulin 7 may have anti-angiogenic activity (3). Fibulin 7 may be important for proper development, since it is included in a chromosomal deletion that is associated with severe cardiac defects and craniofacial anomalies (4).

References

  1. Gallagher, W.M. et al. (2005) Trends Mol. Med. 11:336.
  2. de Vega, S. et al. (2007) J. Biol. Chem. 282:30878.
  3. de Vega, S. et al. (2014) Arch. Biochem. Biophys. 545:148.
  4. Russell, M.W. et al. (2014) Hum. Mol. Genet. 23:4272.

Alternate Names

FBLN7, FIBL-7, TM14

Entrez Gene IDs

129804 (Human); 70370 (Mouse); 296145 (Rat)

Gene Symbol

FBLN7

UniProt

Q501P1

Additional Fibulin 7 Products

Product Documents for Recombinant Mouse Fibulin 7 Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Recombinant Mouse Fibulin 7 Protein, CF

For research use only

Related Research Areas

Extracellular Matrix Molecules

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