Recombinant Mouse FLRT1 His-tag Protein, CF

Catalog # Availability Size / Price Qty
Recombinant Mouse FLRT1 His-tag Protein Bioactivity.
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Product Details

Recombinant Mouse FLRT1 His-tag Protein, CF Summary

Product Specifications

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by the ability of the immobilized protein to support the adhesion of Neuro‑2A mouse neuroblastoma cells. The ED50 for this effect is 0.50-4.00 μg/mL.
Mouse myeloma cell line, NS0-derived mouse FLRT1 protein
Accession #
N-terminal Sequence
Predicted Molecular Mass
60 kDa
65-83 kDa, under reducing conditions.

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity View Larger

Recombinant Mouse FLRT1 His-tag Protein (Catalog # 10955-FL) supports the adhesion of Neuro 2A mouse neuroblastoma cells. The ED50 for this effect is 0.500-4.00 µg/mL.

SDS-PAGE View Larger

2 μg/lane of Recombinant Mouse FLRT1 His-tag Protein (Catalog # 10955-FL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 65-83 kDa.

Reconstitution Calculator

Reconstitution Calculator

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Background: FLRT1

Fibronectin-like domain-containing leucine-rich transmembrane protein 1 (FLRT1) is one of three FLRT leucine-rich repeat transmembrane glycoproteins involved in the regulation of FGF signaling. FLRTs are characterized by an extracellular domain (ECD) with fibronectin and leucine rich repeat (LRR) motifs involved in cell adhesion functions (1). Mature mouse FLRT1 consists of an ECD with 10 N-terminal leucine-rich repeats and a juxtamembrane fibronectin type III domain, a single transmembrane domain and a short cytoplasmic domain lacking defined signaling motifs (1). The ECD of mouse FLRT1 shares 97% amino acid (aa) sequence identity with the ECD of human FLRT1. Both during development and in adulthood, FLRT1 is expressed in distinct areas of the brain and other tissues, including the kidney, and Its expression is distinct from that of FLRT2 and FLRT3 (1, 2). In mouse, FLRT1 is expressed at brain compartmental boundaries in embryos (2). The fibronectin domain of all three FLRTs can bind FGF receptors and this binding is thought to regulate FGF signaling during development (2, 3). The LRR domains are responsible for both the localization of FLRTs in areas of cell contact and homotypic cell-cell association (4). This may be through direct interactions with other FLRT molecules or, as has been shown for FLRT3, by regulating internalization of adhesion molecules such as cadherins (4, 5). Down-regulation of FLRT1 has been associated with prognosis of gastric cancer and has potential to be a therapeutic target for tumor treatment (6).

  1. Lacy, S.E. et al. (1999) Genomics 62:417.
  2. Haines, B.P. et al. (2006) Dev. Biol. 297:14.
  3. Bottcher, R.T. et al. (2004) Nat. Cell Biol. 6:38.
  4. Karaulanov, E.E. et al. (2006) EMBO Rep. 7:283.
  5. Ogata, S. et al. (2007) Genes Dev. 21:1817.
  6. Liang, Y. et al. (2019) Pathol. Res. Pract. 215:152570.
Long Name
Fibronectin Leucine Rich Transmembrane Protein 1
Entrez Gene IDs
23769 (Human); 396184 (Mouse)
Alternate Names
fibronectin leucine rich transmembrane protein 1; Fibronectin-like domain-containing leucine-rich transmembrane protein 1; FLRT1; leucine-rich repeat transmembrane protein FLRT1; MGC21624; SPG68


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