Recombinant Mouse Galectin-3 (Hek293-expressed) Protein, CF

Catalog # Availability Size / Price Qty
9039-GAB-050
Recombinant Mouse Galectin-3 (Hek293-expressed) Protein Bioactivity
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Recombinant Mouse Galectin-3 (Hek293-expressed) Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of D10.G4.1 mouse helper T cells. The ED50 for this effect is 1-5 μg/mL.
Source
Human embryonic kidney cell, HEK293-derived mouse Galectin-3 protein
Met1-Ile264
Accession #
N-terminal Sequence
Analysis
No results obtained
Predicted Molecular Mass
27 kDa
SDS-PAGE
27-36 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9039-GAB

Formulation Lyophilized from a 0.2 μm filtered solution in HEPES, NaCl, TCEP, PEG and Trehalose.
Reconstitution Reconstitute at 200 μg/mL in water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity Recombinant Mouse Galectin-3 (Hek293-expressed) Protein Bioactivity View Larger

Recombinant Mouse Galectin-3 (Catalog # 9039-GAB) supports the adhesion of D10.G4.1 mouse helper T cells. The ED50 for this effect is 1-5 μg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Galectin-3

Galectin-3, also known as Mac-2, L29, CBP35, and epsilon BP, is classified as a chimeric member of the Galectin superfamily and contains one carbohydrate recognition domain (CRD) linked to a non-lectin domain (1, 2). Mature mouse Galectin-3 shares 80% and 86% amino acid (aa) sequence identity with human and rat Galectin-3, respectively. Galectin-3 is a 26-kDa protein that can be nuclear, cytoplasmic, or secreted (3, 4). Nuclear Galectin-3 can modulate gene expression, while cytosolic Galectin-3 can inhibit apoptosis and can participate in exocytosis, Caveolin-mediated endocytosis, and macrophage-mediated clearance of apoptotic cells (5-7). Extracellular Galectin-3 has been shown to form high-order oligomers that promote the crosslinking of cell surface oligosaccharides as well as integrin-dependent cell adhesion and apoptosis (8-11). Galectin-3 contributes to the innate immune response against Candida albicans and Streptococcus pneumoniae, and it can facilitate acute inflammatory responses via neutrophil activation and opsonization, macrophage recruitment, and mast cell activation (12-14). Galectin-3 can also contribute to chronic inflammation and fibrosis (15). It is implicated in neuroinflammatory disorders of the central nervous system, cardiac fibrosis, and heart failure, as well as tumor growth, progression, and metastasis (16-18).

References
  1. Robertson, M.W. et al. (1990) Biochemistry 29:8093.
  2. Elola, M.T. et al. (2007) Cell. Mol. Life Sci. 64:1679.
  3. Haudek, K.C. et al. (2010) Biochim. Biophys. Acta 1800:181.
  4. Hughes, R.C. (1999) Biochim. Biophys. Acta 1473:172.
  5. Krzeslak, A. and A. Lipinska (2004) Cell. Mol. Biol. Lett. 9:305.
  6. Dumic, J. et al. (2006) Biochim. Biophys. Acta 1760:616.
  7. Sano, H. et al. (2003) J. Clin. Invest. 112:389.
  8. Ahmad, N. et al. (2004) J. Biol. Chem. 279:10841.
  9. Friedrichs, J. et al. (2008) J. Biol. Chem. 283:32264.
  10. Wen, Y. et al. (2006) J. Cell. Biochem. 98:115.
  11. Zhuo, Y. et al. (2008) J. Biol. Chem. 283:22177.
  12. Henderson, N.C. and T. Sethi (2009) Immunol. Rev. 230:160.
  13. Kohatsu, L. et al. (2006) J. Immunol. 177:471814.
  14. Nieminen, J. et al. (2008) J. Immunol. 180:2466.
  15. Henderson, N.C. et al. (2008) Am. J. Pathol. 172:288.
  16. Newlaczyl, A.U. and L.G. Yu (2011) Cancer Lett. 313:123.
  17. Sherwi, N. et al. (2012) Future Cardiol. 8:885.
  18. Shin, T. (2013) Acta Histochem. 115:407.
Entrez Gene IDs
3958 (Human); 16854 (Mouse)
Alternate Names
AGE-R3; Carbohydrate-binding protein 35; CBP 35,35 kDa lectin; CBP35; GAL3; Gal-3; galactin-3; Galactose-specific lectin 3; Galactoside-binding protein; GALBPCBP35; Galectin3; Galectin-3; GALIG; IgE-binding protein; L29; L31; L-31; Laminin-binding protein; Lectin L-29; lectin, galactoside-binding, soluble, 3; LGALS2; LGALS3; Mac-2 antigen; Mac-2; MAC2GAL3

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