Recombinant Mouse GDF-9 Protein

(6 citations)   
  • Purity
    >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to induce cell death using Mv1Lu mink lung epithelial cells. The ED50 for this effect is 0.1-0.4 μg/mL.
  • Source
    Chinese Hamster Ovary cell line, CHO-derived Gly307-Arg441
  • Accession #
  • N-terminal Sequence
    Analysis
    Gly307
  • Predicted Molecular Mass
    15.6 kDa
  • SDS-PAGE
    20 kDa, reducing conditions
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
739-G9
 
739-G9/CF
Formulation Lyophilized from a 0.2 μm filtered solution in HCl with BSA as a carrier protein.
Formulation Lyophilized from a 0.2 μm filtered solution in HCl.
Reconstitution Reconstitute at 100 μg/mL in 4 mM HCl containing 0.1% bovine serum albumin.
Reconstitution Reconstitute at 100 μg/mL in 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Recombinant Mouse GDF-9 induces Mv1Lu mink lung epithelial cell death. The ED50 for this effect is 0.1-0.4 μg/mL.
Background: GDF-9
Growth Differentiation Factor-9 (GDF-9) is an oocyte secreted paracrine factor in the TGF-beta superfamily (1, 2). It is synthesized as a prepropeptide and is subsequently processed by proteases into the mature protein (1, 2). Mature mouse GDF-9 has a predicted molecular weight of 15.6 kDa, and shares 90% and 95% amino acid sequence identity with mature human and rat GDF-9, respectively. It forms both non-covalent homodimers and heterodimers with BMP-15, which is coordinately expressed with GDF-9 in the oocyte. (3-5). GDF-9 signals through TGF-beta RI/ALK-5 and BMPR-II, while the GDF-9:BMP-15 heterodimer is believed to signal through BMPR-II, ALK-4, -5, -7, and BMPR-IB/ALK-6 (5-8). SMAD2 and SMAD3 are phosphorylated following activation of receptor complexes by GDF-9 (5, 6). GDF-9 functions as a paracrine factor in the development of primary follicles in the ovary (9, 10). It is critical for the growth of granulosa and theca cells and for the differentiation and maturation of the oocyte (11, 12). GDF-9 is thought to act synergistically with BMP-15 to control development of the oocyte-cumulus cell complex (5, 12-14). In mice, GDF-9:BMP-15 heterodimers have been shown to be more potent regulators of granulosa cell functions compared to GDF-9 homodimers (6). Studies on GDF-9 null mice have demonstrated arrested follicular development at the primary follicle stage (10). In humans, aberrant GDF-9 expression and activation is associated with a multitude of common human ovarian disorders including premature ovarian failure and polycystic ovary syndrome (15-17).
  • References:
    1. McPherron, A.C. and S.J. Lee (1993) J. Biol. Chem. 268:3444.
    2. McGrath, S. A. et al. (1995) Mol. Endocrinol. 9:131.
    3. Laitinen, M. et al. (1998) Mech. Dev. 78:135.
    4. Liao, W.X. et al. (2003) J. Biol. Chem. 278:3713.
    5. Gilchrist, R.B. et al. (2008) Hum. Reprod. Update 14:159.
    6. Peng, J. et al. (2013) Proc. Natl. Acad. Sci. USA 110:E776.
    7. Vitt, U.A. et al. (2002) Biol. Reprod. 67:473.
    8. Mazerbourg, S. et al. (2004) Mol. Endocrinol. 18:653.
    9. Elvin, J.A. et al. (1999) Mol. Endocrinol. 13:1035.
    10. Dong, J. et al. (1996) Nature 383:531.
    11. Hreinsson, J.G. et al. (2002) J. Clin. Endocrinol. Metab. 87:316.
    12. Su, Y.Q. et al. (2004) Dev. Biol. 276:64.
    13. Yan, C. et al. (2001) Mol. Endocrinol. 15:854.
    14. Mottershead, D.G. et al. (2012) Mol. Hum. Reprod. 18:121.
    15. Zhao, S.Y. et al. (2010) Fertil. Steril. 94:261.
    16. Wei, L.N. et al. (2011) Fertil. Steril. 96:464.
    17. Simpson, C.M. et al. (2014) J. Clin. Endocrinol. Metab. [Epub ahead of print].
  • Long Name:
    Growth Differentiation Factor 9
  • Entrez Gene IDs:
    2661 (Human); 14566 (Mouse)
  • Alternate Names:
    GDF9; GDF-9; growth differentiation factor 9; growth/differentiation factor 9
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
Filter your results:

Species
Applications
Sample Type
  1. Natriuretic peptide type C induces sperm attraction for fertilization in mouse
    Authors: N Kong, X Xu, Y Zhang, Y Wang, X Hao, Y Zhao, J Qiao, G Xia, M Zhang
    Sci Rep, 2017;7(0):39711.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  2. Oocyte-dependent activation of MTOR in cumulus cells controls the development and survival of cumulus-oocyte complexes
    J Cell Sci, 2016;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  3. Cumulin, an Oocyte-secreted Heterodimer of the Transforming Growth Factor-beta Family, Is a Potent Activator of Granulosa Cells and Improves Oocyte Quality.
    Authors: Mottershead D, Sugimura S, Al-Musawi S, Li J, Richani D, White M, Martin G, Trotta A, Ritter L, Shi J, Mueller T, Harrison C, Gilchrist R
    J Biol Chem, 2015;290(39):24007-20.
    Species: Human
    Sample Type: Protein
    Application: WB Standard
  4. Bone morphogenetic protein 15 in the pro-mature complex form enhances bovine oocyte developmental competence.
    Authors: Sudiman J, Sutton-McDowall M, Ritter L, White M, Mottershead D, Thompson J, Gilchrist R
    PLoS ONE, 2014;9(7):e103563.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  5. Signalling pathways mediating specific synergistic interactions between GDF9 and BMP15.
    Authors: Mottershead D, Ritter L, Gilchrist R
    Mol Hum Reprod, 2012;18(3):121-8.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  6. Fibroblast growth factors and epidermal growth factor cooperate with oocyte-derived members of the TGFbeta superfamily to regulate Spry2 mRNA levels in mouse cumulus cells.
    Authors: Sugiura K, Su YQ, Li Q, Wigglesworth K, Matzuk MM, Eppig JJ
    Biol. Reprod., 2009;81(5):833-41.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay

Have you used Recombinant Mouse GDF-9 Protein?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

  • Formulations

Contact Information

Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee

Feedback

Customer Information