Recombinant Mouse GFR alpha-3 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
2645-FR-050
R&D Systems Recombinant Proteins and Enzymes
1 Image
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews

Recombinant Mouse GFR alpha-3 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>80%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized Recombinant Mouse Artemin (Catalog # 1085-AR) at 1 µg/mL can bind Recombinant Mouse GFR alpha ‑3/GDNF R alpha ‑3 Fc Chimera with an apparent Kd <1 nM.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived mouse GFR alpha-3/GDNF R alpha-3 protein
Met Mouse GFR alpha -3
(Glu34-Arg379)
Accession # AAB70931
IEGRMD Human IgG1
(Pro100-Lys330)
6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Met
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
66.2 kDa (monomer)
SDS-PAGE
70-75 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2645-FR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS and NaCl.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: GFR alpha-3/GDNF R alpha-3

Mouse glial cell line-derived neurotrophic factor (GDNF) family receptor alpha 3 (GFR alpha -3, GDNF R alpha -3) is an approximately 50 kDa plasma membrane glycoprotein that is one of four GDNF receptors, all of which are glycosylphosphoinositol (GPI)-anchored, contain three conserved cysteine repeats, and promote survival of neurons (1‑4). It is synthesized as a 397 aa precursor with a 28 aa N‑terminal signal sequence, a 343 aa mature segment, and a C-terminal GPI attachment signal sequence (3‑5). Mouse GFR alpha -3 shares 94% aa sequence identity with rat GFR alpha -3 and 76‑81% aa sequence identity with human, equine, bovine and canine GFR alpha -3. GFR alpha -3 is expressed in the central nervous system only during the earliest stages of neurogenesis, while later it is predominantly found in developing and adult nociceptive sensory neurons (2, 4‑8). It is expressed with the shared GDNF co‑receptor, the Ret receptor tyrosine kinase, in the trigeminal ganglion, pituitary gland, thymus, lung, and duodenum (2, 4). Its ligand, the GDNF family ligand artemin, is primarily expressed by immature Schwann cells in the peripheral nervous system, and vascular smooth muscle cells, directing axonal projection of sympathetic neurons (6, 7). Artemin first binds GFR alpha -3, which recruits Ret, forming a signaling complex that is a pentamer containing one artemin, two GFR alpha -3, and two Ret molecules (1, 6, 9). Signals from this complex are required for the development and survival of the superior cervical ganglion (SCG) neurons, and deletion of mouse GFR alpha -3 results in deficits in the SCG and inhibited development of intestinal Peyer’s patches (6, 7, 10, 11).

References
  1. Wang, X. et al. (2006) Structure 14:1083.
  2. Naveilhan, P. et al. (1998) Proc. Natl. Acad. Sci. USA 95:1295.
  3. Nomoto, S. et al. (1998) Biochem. Biophys. Res. Commun. 244:849.
  4. Baloh, R. et al. (1998) Proc. Natl. Acad. Sci. USA 95:5801.
  5. Worby, C. et al. (1998) J. Biol. Chem. 273:3502.
  6. Baloh, R. et al. (1998) Neuron 21:1291.
  7. Honma, Y. et al. (2002) Neuron 35:267.
  8. Orozco, O.E. et al.(2001) Eur. J. Neurosci. 13:2177.
  9. Schlee, S. et al. (2006) Nat. Chem. Biol. 2:636.
  10. Nishino, J et al. (1999) Neuron 23:725.
  11. Veiga-Fernandez, H. et al. (2007) Nature 446:547.
Long Name
Glial Cell line-derived Neurotrophic Factor Receptor alpha 3
Entrez Gene IDs
2676 (Human); 14587 (Mouse)
Alternate Names
GDNF family receptor alpha 3; GDNF family receptor alpha-3; GDNF R alpha-3; GDNF receptor alpha-3; GDNFR-alpha-3; GFR alpha3; GFR alpha-3; GFRA3; GFRa-3; GFR-alpha-3; glial cell line-derived neurotrophic factor receptor alpha-3; GPI-linked receptor

Citation for Recombinant Mouse GFR alpha-3 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Autocrine regulation of early embryonic development by the artemin-GFRA3 (GDNF family receptor-alpha 3) signaling system in mice.
    Authors: Li J, Klein C, Liang C, Rauch R, Kawamura K, Hsueh AJ
    FEBS Lett., 2009;583(15):2479-85.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Recombinant Proteins

Reconstitution Buffers

Reviews for Recombinant Mouse GFR alpha-3 Fc Chimera Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Mouse GFR alpha-3 Fc Chimera Protein, CF and earn rewards!

Have you used Recombinant Mouse GFR alpha-3 Fc Chimera Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review