Recombinant Mouse Granzyme G Protein, CF

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R&D Systems Recombinant Proteins and Enzymes
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Product Details

Recombinant Mouse Granzyme G Protein, CF Summary

Product Specifications

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Measured by its ability to cleave a peptide substrate, Succinyl-Phe-Leu-Phe-ThioBenzyl ester (Suc-FLF-SBzl), in the presence of 5,5’-Dithio-bis (2-nitrobenzoic acid) (DTNB). Edwards, K.M. et al. (1999) J. Biol. Chem. 274:30468. The specific activity is >150 pmol/min/µg, as measured under the described conditions.
Spodoptera frugiperda, Sf 21 (baculovirus)-derived mouse Granzyme G protein
Ile21-Leu248, with a C-terminal 10-His tag
Accession # P13366
Accession #
N-terminal Sequence
Structure / Form
Mature form
Predicted Molecular Mass
27 kDa
35 kDa, reducing conditions

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Assay Procedure

  • Assay Buffer: 0.1 M Tris, pH 8.0
  • Recombinant Mouse Granzyme G (rmGranzyme G) (Catalog # 1346-SE)
  • Substrate: SUC-Phe-Leu-Phe-SBZL (Bachem, Catalog # M-1740), 10 mM in DMSO
  • 5,5’-dithio-bis (2-nitrobenzoic acid) (DTNB) (Sigma, Catalog # D-8130), 10 mM in DMSO
  • 96 well Clear Plate (Costar, Catalog # 92592)
  • Plate Reader (Model: SpectraMax Plus by Molecular Devices) or equivalent
  1. Dilute rmGranzyme G to 2 ng/µL in Assay Buffer.
  2. Combine equal volumes of Substrate and DTNB for a final concentration of 5 mM of each.
  3. Dilute the Substrate/DTNB mixture to 200 µM in Assay Buffer.
  4. Load 50 µL of the diluted rmGranzyme G into a 96 well clear plate. Include a Substrate Blank containing 50 µL of Assay Buffer.
  5. Start the reaction by adding 50 µL of the diluted Substrate/DTNB mixture to wells.
  6. Read at a wavelength of 405 nm (bottom read) in kinetic mode for 5 minutes.
  7. Calculate specific activity:

     Specific Activity (pmol/min/µg) =

Adjusted Vmax* (OD/min) x well volume (L) x 1012 pmol/mol
ext. coeff** (M-1cm-1) x path corr.*** (cm) x amount of enzyme (µg)

     *Adjusted for Substrate Blank 
     **Using the extinction coefficient 13260 M-1cm-1 
     ***Using the path correction 0.32 cm
     Note: the output of many spectrophotometers is in mOD. Per Well:
  • rmGranzyme G: 0.1 µg
  • Substrate: 100 µM
  • TNB: 100 µM
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Background: Granzyme G

Granzyme G is a member of the Granzyme family of the serine proteases found specifically in the cytotoxic granules of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells (1). Together with Granzymes D, E and F, it is regulated through pregnancy and by IL-2 and IL-15 in granulated metrial gland cells (2). Human or rat counterpart of mouse Granzyme G has not been found. Like other Granzymes, mouse Granzyme G is not secreted as a zymogen but stored as a fully processed and activated enzyme in the cytoplasmic granules of CTL (3). It is synthesized as a 248 amino acid precursor with a 18 amino acid signal peptide and a 2 amino acid propeptide (3, 4). The mature protein (residues 21‑248) is expressed and purified.

  1. Smyth, M.J. et al. (1996) J. Leukoc. Biol. 60:555.
  2. Allen, M.P. and M. Nilsen-Hamilton (1998) J. Immunol. 161:2772.
  3. Jenne, D.E. et al. (1989) Biochemistry 28:7953.
  4. Kwon, B.S. et al. (1988) J. Exp. Med. 168:1839.
Entrez Gene IDs
14944 (Mouse); 266704 (Rat)
Alternate Names
Ctla7; Ctla-7; Granzyme G; GrzG; GZMG


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