Recombinant Mouse IFN-alpha 11 Protein, CF

R&D Systems | Catalog # 10329-IF

R&D Systems
100% Guarantee

Key Product Details

  • R&D Systems HEK293-derived Recombinant Mouse IFN-alpha 11 Protein (10329-IF)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

HEK293

Accession Number

Q61716.2

Applications

Bioactivity
View all IFN-alpha 11 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived mouse IFN-alpha 11 protein
Cys24-Ala190

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Cys24

Predicted Molecular Mass

19 kDa

SDS-PAGE

18-24 kDa, under reducing conditions

Activity

Measured in an anti-viral assay using L‑929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. Vogel, S.N. et al. (1982) Infect. Immunol. 38:681.
The ED50 for this effect is <20 pg/mL.

Scientific Data Images for Recombinant Mouse IFN-alpha 11 Protein, CF

Recombinant Mouse IFN-alpha 11 Protein Bioactivity

Recombinant Mouse IFN-alpha 11 Protein Bioactivity

Recombinant Mouse IFN-alpha 11 Protein (Catalog # 10329-IF) suppresses viral activity on L-929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is <20 pg/mL.
Recombinant Mouse IFN-alpha 11 Protein SDS-PAGE

Recombinant Mouse IFN-alpha 11 Protein SDS-PAGE

2 μg/lane of Recombinant Mouse IFN-alpha 11 (Catalog # 10329-IF) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 18-24 kDa.

Formulation, Preparation, and Storage

10329-IF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: IFN-alpha 11

Interferons (IFN) are a family of cytokines with potent antiviral, antiproliferative and immunomodulatory properties, classified based on their binding specificity to cell surface receptors (1). There are more than a dozen closely related IFN alpha subtypes found in both the human and mouse genome, each sharing about 80% amino acid (aa) sequence homology (2, 3). The extracellular domain (ECD) of mature mouse IFNA11 shares 81% amino acid sequence identity with rat IFNA11. The closest homolog of IFNA11 identified in human is IFNA5. The type I IFNs bind to the interferon alpha receptor (IFNAR), which consists of two subunits: IFNAR1 (alpha ‑subunit) and IFNAR2 (beta ‑subunit) (4, 5). Individual IFN alpha subtypes are known to display unique efficacies to viral protection, with IFNA11 showing high activity when compared to other subtypes (4). IFNA11 stimulates NK cells resulting in activation and improved effector functions. Treatment with IFNA11 inhibits the FV-replication in acutely infected mice. IFNA11 is not only effective in retroviral infections but also is effective in herpes virus infections. IFNA11 has a potential as an efficient drug for treatment of many different viral diseases (6).

References

  1. Pestka, S. et al. (1987) Annu Rev. Biochem. 56:727.
  2. Matsumiya, T. et al. (2007) J. Immunol. 179:4542.
  3. Schreiber, G. and J. Piehler (2015) Trends Immunol. 36:139.
  4. van Pesch, V. et al. (2004) J. Virol. 78:8219.
  5. James C.M. et al. (2007) Vaccine. 25(10):1856.
  6. Gibbert K. et al. (2012) PLoSPathogens 8:1.

Long Name

Interferon alpha 11

Alternate Names

IFNA11, IFNalpha 11

Entrez Gene IDs

15964 (Mouse); 298211 (Rat)

Gene Symbol

IFNA11

UniProt

Q61716.2

Additional IFN-alpha 11 Products

Product Documents for Recombinant Mouse IFN-alpha 11 Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Mouse IFN-alpha 11 Protein, CF

For research use only

Related Research Areas

Interferons

Customer Reviews for Recombinant Mouse IFN-alpha 11 Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Mouse IFN-alpha 11 Protein, CF and earn rewards!

Have you used Recombinant Mouse IFN-alpha 11 Protein, CF?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Proteins and Enzymes