Recombinant Mouse IFN-alpha 12 Protein, CF Summary
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 100 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Recombinant Mouse IFN-alpha 12 (Catalog # 10370-IF) suppresses viral activity on L-929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is 10-100 pg/mL.
2 μg/lane of Recombinant Mouse IFN-alpha 12 (Catalog # 10370-IF) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 18-25 kDa.
Background: IFN-alpha 12
Interferon-alpha 12 (IFN alpha -12) is a member of the type I interferon-alpha (IFN-alpha ) family (1). The members of the IFN-alpha family, also known as alpha leukocyte interferons, encompass a group of distinct but closely related proteins which share approximately 80% amino acid (aa) sequence identity and have a similar globular structure composed of five alpha-helices (1-3). IFN-alpha family members signal through a common set of cell surface receptor complex composed of IFN-alpha R2 and IFN-alpha R1 subunits (2). The mature extracellular domain (ECD) of mouse IFN alpha -12 is 166 aa. While IFNa-12 does not exist in humans, it closely resembles other murine members of the IFN-alpha family, such as sharing 88% and 86% sequence identity with IFN alpha -15 and IFN alpha -11 respectively. Murine IFN-alpha 12 differs from other members of the IFN-alpha family by its anti-proliferative function and in the inability of its expression to be regulated by viral infection (5).
- Moulton, V. R. (2017) Trends Mol Med. 23:615.
- Oritani, K. et al. (2001) Cytokine & Growth Factor Reviews. 12:337.
- Pesch, V. et al. (2004). Journal of Virology. 78:8219.
- Hardy, M. P. et al. (2004). Genomics, 84:331.
- Tsang, S. L. et al. (2007). Cytokine, 37:101.
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