Interferon-alpha (IFN-alpha), also known as leukocyte interferon, represents a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon family which share a common cell surface receptor composed of two subunits, a 100 kDa ligand-binding subunit (IFN-alpha R2) and a 125 kDa ligand binding and signal transduction subunit (IFN-alpha R1) that is involved both in ligand binding and signal transduction. IFN-alpha has both anti-viral and immunomodulatory activities on target cells.
Recombinant Mouse IFN-alpha 4 (Mammalian) Protein
R&D Systems | Catalog # 12115-1
New Version Available:
10259-IF
NEW
A New rmIFN-alpha 4 is Available! It has lower endotoxin specifications, is made in-house, and is priced competitively!
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Key Product Details
- R&D Systems N/A-derived Recombinant Mouse IFN-alpha 4 (Mammalian) Protein (12115-1)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Accession Number
Applications
Bioactivity
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Product Specifications
Source
Murine (C57/BI) DNA expressed in mammalian cells
Accession # NM_010504
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Predicted Molecular Mass
19.5 kDa
Activity
Interferon was titrated with the use of the cytopathic effect inhibition assay as
described [Familletti
et al. (1981) "A Convenience and Rapid Cytopathic Effect Inhibition Assay for Interferon," in Methods
in Enzymology
, Vol. 78 (S. Pestka, ed.), Academic Press, New York, 387-394] with the exception that EMCV rather than
VSV was used as the challenge. The activity was determined relative to a lab standard of Mu-IFN-alpha A which was
calibrated to the NIH Murine IFN-alpha standard (Ga02-901-511). Mouse (L929/EMCV) in this assay the EC50 for IFN is ~5
U/ml. Lot Activity was derived from multiple determinations in the above assay. Please note that IFN assays vary between
labs and assay systems [Meager
et al (2001). J. Immunol. Meth. 257:17. Meager and Das (2005) J. Immunol Meth. 306:1]
Formulation, Preparation, and Storage
12115-1
| Formulation | Phosphate buffered saline (PBS) containing 0.1% bovine serum albumin (BSA) |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date. |
Background: IFN-alpha 4
Long Name
Interferon alpha 4
Alternate Names
IFNA4, IFNalpha 4
Gene Symbol
IFNA4
UniProt
Additional IFN-alpha 4 Products
Product Documents for Recombinant Mouse IFN-alpha 4 (Mammalian) Protein
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Mouse IFN-alpha 4 (Mammalian) Protein
For research use only
Related Research Areas
Citations for Recombinant Mouse IFN-alpha 4 (Mammalian) Protein
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