Recombinant Mouse IFN-alpha 6/IFNA6 Protein, CF

Catalog # Availability Size / Price Qty
10207-IF-010
10207-IF-050
Recombinant Mouse IFN-alpha 6/IFNA6 Protein, CF Bioactivity
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Recombinant Mouse IFN-alpha 6/IFNA6 Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in an anti-viral assay using L‑929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. Vogel, S.N. et al. (1982) Infect. Immunol. 38:681. The ED50 for this effect is <100 pg/mL.
Source
Human embryonic kidney cell, HEK293-derived mouse IFN-alpha 6/IFNA6 protein
Cys24-Glu189
Accession #
N-terminal Sequence
Analysis
Cys24
Predicted Molecular Mass
19 kDa
SDS-PAGE
19-22 kDa, under reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10207-IF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Bioactivity Bioactivity View Larger

Recombinant Mouse IFN-alpha 6/IFN6 (Catalog # 10207-IF) suppresses viral activity on L-929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is 100 pg/mL

SDS-PAGE SDS-PAGE View Larger

2 μg/lane of Recombinant Mouse IFN-alpha 6/IFNA6 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 19-22 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IFN-alpha 6/IFNA6

Interferons (IFN) are a family of cytokines with potent antiviral, antiproliferative and immunomodulatory properties, classified based on their binding specificity to cell surface receptors (1). There are more than a dozen closely related IFN alpha subtypes found in both the human and mouse genome, each sharing about 80% amino acid (aa) sequence homology (2, 3). Mature mouse IFNA6 consists of 166 aa and shares 60% aa identity with human IFNA6. The type I IFNs binds to the interferon alpha receptor (IFNAR) which consists of two subunits: IFNAR1 (alpha -subunit) and IFNAR2 (beta -subunit) (4, 5). Individual IFN alpha subtypes are known to display unique efficacies to viral protection, with IFNA6 displaying the superior efficacy controlling influenza virus infection and disease (6). Treatment with IFNA6 DNA 2 weeks post‐MCMV infection proved effective at inhibiting the development of chronic autoimmune myocarditis. IFNA6 is also able to reduce chronic cardiac inflammation. These findings suggest that immunomodulation of both antiviral and autoimmune responses by IFN DNA immunization may be an avenue for improved viral immunotherapy (7, 8).

References
  1. Pestka, S. et al. (1987) Annu Rev Biochem. 56:727.
  2. Matsumiya, T. et al. (2007) J. Immunol. 179:4542.
  3. Schreiber, G. and J. Piehler (2015) Trends Immunol. 36:139.
  4. Fung, K.Y. et al. (2013) Science 339:1088.
  5. van Pesch, V. et al. (2004) J. Virol. 78:8219.
  6. James, C.M. et al. (2007) Vaccine. 25(10):1856.
  7. Bartlett, E.J. et al. (2002) Immunol. Cell Biol. 5:425.
  8. Bartlett, E.J. et al. (2003) Biol. Proced. 5:43.
Long Name
Interferon alpha 6
Entrez Gene IDs
3443 (Human); 15969 (Mouse)
Alternate Names
IFNA6; IFN-alpha 6; IFNalpha K; IFN-alpha K; IFN-kappa; interferon kappa; interferon, kappa; RP11-27J8.1; UNQ6124/PRO20084

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