Recombinant Mouse IFN-alpha 7/IFNA7 Protein, CF Summary
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 100 μg/mL in PBS.|
|Shipping||The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Recombinant Mouse IFN-alpha 7/IFNA7 (Catalog # 10208-IJ) suppresses viral activity on L-929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is 1.5 ng/mL.
2 μg/lane of Recombinant Mouse IFN-alpha J1/IFNA7 (Catalog # 10208-IJ) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 18-24 kDa.
Background: IFN-alpha 7/IFNA7
Interferons (IFN) are a family of cytokines with potent antiviral, antiproliferative and immunomodulatory properties, classified based on their binding specificity to cell surface receptors (1). There are more than a dozen closely related IFN alpha subtypes found in both the human and mouse genome, each sharing about 80% amino acid (aa) sequence homology (2, 3). Mature mouse IFNA7 consists of 166 aa and shares 59% identity with human IFNA7. The type I IFNs bind to the interferon alpha receptor (IFNAR), which consists of two subunits: IFNAR1 (alpha -subunit) and IFNAR2 (beta -subunit) (4, 5). Individual IFN alpha subtypes are known to display unique efficacies to viral protection, with IFNA7 showing slightly lower antiviral potency against Mengo virus but maintaining similar anti-proliferative potency in B16 melanoma cells (5, 6).
- Pestka S, et al. (1987) Annu Rev Biochem. 56:727.
- Matsumiya, T. et al. (2007) J. Immunol. 179:4542.
- Schreiber, G. and J. Piehler (2015) Trends Immunol. 36:139.
- Fung, K.Y. et al. (2013) Science 339:1088.
- van Pesch, V. et al. (2004) J. Virol. 78:8219.
- James CM et al. (2007) Vaccine. 25(10):1856.
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