Recombinant Mouse IGFBP-2 Protein, CF

Catalog # Availability Size / Price Qty
797-B2-025
Product Details
Citations (5)
FAQs
Reviews (1)

Recombinant Mouse IGFBP-2 Protein, CF Summary

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit the biological activity of IGF-I or IGF-II on MCF‑7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083. The ED50 for this effect is 0.125‑0.5 µg/mL in the presence of 30 ng/mL rmIGF-II.
Source
Mouse myeloma cell line, NS0-derived mouse IGFBP-2 protein
Gln35-Gln305
Accession #
N-terminal Sequence
Analysis
Gln35
Predicted Molecular Mass
29.4 kDa
SDS-PAGE
35 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

797-B2

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IGFBP-2

The superfamily of insulin-like growth factor (IGF) binding proteins include the six high-affinity IGF binding proteins (IGFBP) and at least four additional low-affinity binding proteins referred to as IGFBP related proteins (IGFBP-rP). All IGFBP superfamily members are cysteine-rich proteins with conserved cysteine residues, which are clustered in the amino- and carboxy-terminal thirds of the molecule. IGFBPs modulate the biological activities of IGF proteins. Some IGFBPs may also have intrinsic bioactivity that is independent of their ability to bind IGF proteins. Post-translational modifications of IGFBPs, including glycosylation, phosphorylation and proteolysis, have been shown to modify the affinities of the binding proteins to IGF.

Mouse IGFBP-2 cDNA encodes a 305 amino acid (aa) residue precursor protein with a putative 34 aa residue signal peptide that is processed to generate the 271 aa residue mature protein. Mouse and human IGFBP-2 share approximately 82% homology. IGFBP-2 contains an integrin receptor recognition sequence (RGD sequence) but lacks potential N-linked glycosylation sites. During development, IGFBP-2 is expressed in a number of tissues. The highest expression level is found in the central nervous system. In adults, high expression levels are also detected in the central nervous system and in a number of reproductive tissues. IGFBP-2 binds preferentially to IGF II, exhibiting a 2 - 10 fold higher affinity for IGF II than for IGF I.

References
  1. Jones, J.I. and D.R. Clemmons (1995) Endocrine Rev. 16:3.
  2. Kelley, K.M. et al. (1996) Int. J. Biochem. Cell Biol. 28:619.
  3. Schuller, A.G.P. et al. (1994) Mol. Cell. Endoc. 104:57.
  4. Landwehr, J. et al. (1993) Gene 124:281.
Long Name
Insulin-like Growth Factor Binding Protein 2
Entrez Gene IDs
3485 (Human); 16008 (Mouse)
Alternate Names
BP2; IBP2; IBP-2; IGF-binding protein 2; IGFBP2; IGFBP-2; IGF-BP53; insulin-like growth factor binding protein 2 (36kD); insulin-like growth factor binding protein 2, 36kDa; insulin-like growth factor-binding protein 2

Citations for Recombinant Mouse IGFBP-2 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Tbx3-dependent amplifying stem cell progeny drives interfollicular epidermal expansion during pregnancy and regeneration
    Authors: R Ichijo, H Kobayashi, S Yoneda, Y Iizuka, H Kubo, S Matsumura, S Kitano, H Miyachi, T Honda, F Toyoshima
    Nat Commun, 2017;8(1):508.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  2. Neural deletion of Tgfbr2 impairs angiogenesis through an altered secretome.
    Authors: Hellbach N, Weise S, Vezzali R, Wahane S, Heidrich S, Roidl D, Pruszak J, Esser J, Vogel T
    Hum Mol Genet, 2014;23(23):6177-90.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Patched1 inhibits epidermal progenitor cell expansion and basal cell carcinoma formation by limiting Igfbp2 activity.
    Authors: Villani RM, Adolphe C, Palmer J, Waters MJ, Wainwright BJ
    Cancer Prev Res (Phila), 2010;3(10):1222-34.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  4. Evidence of a role for insulin-like growth factor binding protein (IGFBP)-3 in metabolic regulation.
    Authors: Yamada PM, Mehta HH, Hwang D
    Endocrinology, 2010;151(12):5741-50.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)
  5. Dietary feeding of silibinin inhibits prostate tumor growth and progression in transgenic adenocarcinoma of the mouse prostate model.
    Authors: Raina K, Blouin MJ, Singh RP, Majeed N, Deep G, Varghese L, Glode LM, Greenberg NM, Hwang D, Cohen P, Pollak MN, Agarwal R
    Cancer Res., 2007;67(22):11083-91.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)

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Reviews for Recombinant Mouse IGFBP-2 Protein, CF

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Recombinant Mouse IGFBP-2 Protein, CF
By Anonymous on 10/21/2016
Application: CellProlif