Recombinant Mouse IGSF4B/SynCAM3 His-tag Protein, CF Summary
Asn23-His328, with a C-terminal 6-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 1 mg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||
2 μg/lane of Recombinant Mouse IGSF4B/SynCAM3 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 35-44 kDa.
IGSF4B (immunoglobulin superfamily member 4B), also called CADM3, TSLL1, Necl-1 and SynCAM-3, is a neural tissue-specific member of the nectin-like family of immunoglobulin superfamily (1, 2). It is a 48-50 kDa type I transmembrane (TM) glycoprotein that is concentrated at non-junctional contact sites of neuronal axons and glial processes (2, 3). In myelinated peripheral nerve fibers, IGSF4B is concentrated at nodes of Ranvier in contact sites of Schwann cells (2). The 396 amino acid (aa) mouse IGSF4B contains a 22 aa signal sequence, a 306 aa extracellular domain (ECD), a 21 aa transmembrane domain and a 47 aa cytoplasmic domain. The IGSF4B ECD is highly conserved, sharing 95-96% aa identity between human, mouse, rat, canine and bovine sequences. The ECD of IGSF4A, B, C and D proteins share 35-50% aa identity. The ECD contains an N-terminal V-type Ig-like domain that is responsible for Ca2+‑independent homophilic and heterophilic interactions with Nectin-1, Nectin-3 or Necl-2 (IGSF-4) in trans. It also contains two C2‑type Ig-like domains that are responsible for Ca2+-independent homophilic dimerization in cis that is thought to precede trans interaction (2, 4, 5). The cytoplasmic domain binds members of the MAGUK guanylate kinase subfamily, such as Dlg3, Pals2 and CASK (2). These activities are thought to play roles in adhesion and architecture at the synapse (2). IGSF-4B was also identified as a tumor-suppressor gene and it inhibits migration, invasion, and induces differentiation of glioma cells (6). It also suppresses growth and tumorigenic ability of colon cancer cells (7). We have also shown that IGSF4B promotes in vitro outgrowth of cortical neurons.
- Fukuhara, H. et al. (2001) Oncogene 20:5401.
- Kakunaga, S. et al. (2005) J. Cell Sci. 118:1267.
- Fukami, T. et al. (2003) Gene 323:11.
- Shingai, T. et al. (2003) J. Biol. Chem. 278:35421.
- Dong, X. et al. (2006) J. Biol. Chem. 281:10610.
- Yin B. et al. (2009) Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 31:669.
- Raveh S. et al. (2009) J. Cell Biochem. 108:326.
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