Recombinant Mouse IGSF9 Protein, CF

  
  • Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to inhibit neurite outgrowth of dissociated E13 chick embryonic dorsal root ganglia (DRG) neurons. Able to significantly inhibit neurite outgrowth when immobilized as a 3 µL droplet containing 150 ng on a nitrocellulose-coated microplate
  • Source
    Mouse myeloma cell line, NS0-derived Arg21-Leu730, with a C-terminal 6-His tag
  • Accession #
  • N-terminal Sequence
    Analysis
    Arg21
  • Predicted Molecular Mass
    78 kDa
  • SDS-PAGE
    95-110 kDa, reducing conditions
9140-S9
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Recombinant Mouse IGSF9 (Catalog #
9140-S9) Inhibits Neurite Outgrowth in E13 Chicken Embryonic Dorsal Root Ganglia (DRG) Neurons. A) Normal Neurite outgrowth is observed in untreated chicken E13 DRG neurons. B) Chicken DRG neurite outgrowth is significantly inhibited when Recombinant Mouse IGSF9 is immobilized as a 3 μL droplet containing 150 ng of IGSF9 protein on a nitrocellulose-coated microplate.
Background: IGSF9
IGSF9, also known as Dasm1, is an approximately 130 kDa transmembrane protein that plays a role in neuronal synapse maintenance and function (1). Mature mouse Dasm1 consists of a 714 amino acid (aa) extracellular domain (ECD) with 5 Ig-like domains and 2 fibronectin type-3 domains, a 21 aa transmembrane segment, and a 424 aa cytoplasmic domain (2). Within the ECD, mouse Dasm1 shares 90% and 96% aa sequence identity with human and rat Dasm1, respectively. Alternative splicing generates an additional isoform that is truncated following the fifth Ig-like domain. Dasm1 is expressed in the dorsal root and trigeminal ganglia, forebrain, cortex, dentate gyrus, pyramidal cells, Purkinje cells, and hippocampal CA1 interneurons (2-5). It localizes to dendrites, cell bodies, and post-synaptic densities (3, 6). Dasm1 functions as a homophilic adhesion protein that supports the maintenance of inhibitory synapses as well as inhibitory neurotransmission (5, 6).
  • References:
    1. Hansen, M. and P.S. Walmod (2013) Neurochem. Res. 38:1236.
    2. Doudney, K. et al. (2002) Genomics 79:663.
    3. Shi, S.-H. et al. (2004) Proc. Natl. Acad. Sci. USA 101:13341.
    4. Mishra, A. et al. (2008) Mol. Cell. Biol. 28:2782.
    5. Mishra, A. et al. (2014) J. Neurosci. 34:4187.
    6. Shi, S.-H. et al. (2004) Proc. Natl. Acad. Sci. USA 101:13346.
  • Long Name:
    Immunoglobulin Superfamily, Member 9
  • Entrez Gene IDs:
    57549 (Human); 93842 (Mouse); 304982 (Rat)
  • Alternate Names:
    Dasm1; FP18798; IGSF9; IGSF9A; immunoglobulin superfamily member 9A; immunoglobulin superfamily, member 9; KIAA1355; Nrt1; Protein Turtle Homolog A; TUTLA
Related Research Areas
Primary Antibodies
Description Application Cat# Citations Images  

His Tag Antibody

WB, Flow, AP MAB050R  
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His Tag Antibody

WB, Simple Western, Flow, AP, CyTOF-ready MAB050 20  
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His Tag Horseradish Peroxidase-conjugated Antibody

WB, Simple Western MAB050H 3  
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His Tag APC-conjugated Antibody

ICFlow IC050A  
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His Tag PE-conjugated Antibody

ICFlow IC050P 1  
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His Tag Biotinylated Antibody

WB BAM050 1  
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His Tag Alexa Fluor® 488-conjugated Antibody

ICFlow IC050G  
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His Tag PerCP-conjugated Antibody

ICFlow IC050C  
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His Tag Alexa Fluor® 700-conjugated Antibody

ICFlow IC050N  
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Protein Purification Kits
Description Application Cat# Citations Images  

Histidine-tagged Protein Purification Resin

IP999
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