Recombinant Mouse IGSF9 Protein, CF Summary
Arg21-Leu730, with a C-terminal 6-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 250 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Recombinant Mouse IGSF9 (Catalog # 9140-S9) Inhibits Neurite Outgrowth in E13 Chicken Embryonic Dorsal Root Ganglia (DRG) Neurons. A) Normal Neurite outgrowth is observed in untreated chicken E13 DRG neurons. B) Chicken DRG neurite outgrowth is significantly inhibited when Recombinant Mouse IGSF9 is immobilized as a 3 μL droplet containing 150 ng of IGSF9 protein on a nitrocellulose-coated microplate.
IGSF9, also known as Dasm1, is an approximately 130 kDa transmembrane protein that plays a role in neuronal synapse maintenance and function (1). Mature mouse Dasm1 consists of a 714 amino acid (aa) extracellular domain (ECD) with 5 Ig-like domains and 2 fibronectin type-3 domains, a 21 aa transmembrane segment, and a 424 aa cytoplasmic domain (2). Within the ECD, mouse Dasm1 shares 90% and 96% aa sequence identity with human and rat Dasm1, respectively. Alternative splicing generates an additional isoform that is truncated following the fifth Ig-like domain. Dasm1 is expressed in the dorsal root and trigeminal ganglia, forebrain, cortex, dentate gyrus, pyramidal cells, Purkinje cells, and hippocampal CA1 interneurons (2-5). It localizes to dendrites, cell bodies, and post-synaptic densities (3, 6). Dasm1 functions as a homophilic adhesion protein that supports the maintenance of inhibitory synapses as well as inhibitory neurotransmission (5, 6).
- Hansen, M. and P.S. Walmod (2013) Neurochem. Res. 38:1236.
- Doudney, K. et al. (2002) Genomics 79:663.
- Shi, S.-H. et al. (2004) Proc. Natl. Acad. Sci. USA 101:13341.
- Mishra, A. et al. (2008) Mol. Cell. Biol. 28:2782.
- Mishra, A. et al. (2014) J. Neurosci. 34:4187.
- Shi, S.-H. et al. (2004) Proc. Natl. Acad. Sci. USA 101:13346.
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