Recombinant Mouse IGSF9 Protein, CF

Catalog # Availability Size / Price Qty
9140-S9-050
Recombinant Mouse IGSF9 Protein, CF Data
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Recombinant Mouse IGSF9 Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit neurite outgrowth of dissociated E13 chick embryonic dorsal root ganglia (DRG) neurons. Able to significantly inhibit neurite outgrowth when immobilized as a 3 µL droplet containing 150 ng on a nitrocellulose-coated microplate
Source
Mouse myeloma cell line, NS0-derived mouse IGSF9 protein
Arg21-Leu730, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Arg21
Predicted Molecular Mass
78 kDa
SDS-PAGE
95-110 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9140-S9

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity View Larger

Recombinant Mouse IGSF9 (Catalog # 9140-S9) Inhibits Neurite Outgrowth in E13 Chicken Embryonic Dorsal Root Ganglia (DRG) Neurons. A) Normal Neurite outgrowth is observed in untreated chicken E13 DRG neurons. B) Chicken DRG neurite outgrowth is significantly inhibited when Recombinant Mouse IGSF9 is immobilized as a 3 μL droplet containing 150 ng of IGSF9 protein on a nitrocellulose-coated microplate.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IGSF9

IGSF9, also known as Dasm1, is an approximately 130 kDa transmembrane protein that plays a role in neuronal synapse maintenance and function (1). Mature mouse Dasm1 consists of a 714 amino acid (aa) extracellular domain (ECD) with 5 Ig-like domains and 2 fibronectin type-3 domains, a 21 aa transmembrane segment, and a 424 aa cytoplasmic domain (2). Within the ECD, mouse Dasm1 shares 90% and 96% aa sequence identity with human and rat Dasm1, respectively. Alternative splicing generates an additional isoform that is truncated following the fifth Ig-like domain. Dasm1 is expressed in the dorsal root and trigeminal ganglia, forebrain, cortex, dentate gyrus, pyramidal cells, Purkinje cells, and hippocampal CA1 interneurons (2-5). It localizes to dendrites, cell bodies, and post-synaptic densities (3, 6). Dasm1 functions as a homophilic adhesion protein that supports the maintenance of inhibitory synapses as well as inhibitory neurotransmission (5, 6).

References
  1. Hansen, M. and P.S. Walmod (2013) Neurochem. Res. 38:1236.
  2. Doudney, K. et al. (2002) Genomics 79:663.
  3. Shi, S.-H. et al. (2004) Proc. Natl. Acad. Sci. USA 101:13341.
  4. Mishra, A. et al. (2008) Mol. Cell. Biol. 28:2782.
  5. Mishra, A. et al. (2014) J. Neurosci. 34:4187.
  6. Shi, S.-H. et al. (2004) Proc. Natl. Acad. Sci. USA 101:13346.
Long Name
Immunoglobulin Superfamily, Member 9
Entrez Gene IDs
57549 (Human); 93842 (Mouse); 304982 (Rat)
Alternate Names
Dasm1; FP18798; IGSF9; IGSF9A; immunoglobulin superfamily member 9A; immunoglobulin superfamily, member 9; KIAA1355; Nrt1; Protein Turtle Homolog A; TUTLA

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