Recombinant Mouse IL-10 R beta Fc Chimera Protein, CF Summary
|Mouse IL-10 R beta
Accession # Q61190
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS and Trehalose.|
|Reconstitution||Reconstitute at 100 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Background: IL-10 R beta
Interleukin-10 Receptor beta (IL-10 R beta ), also known as IL-10 R2 and CRF2-4, is a 60 kDa transmembrane glycoprotein that functions as a co-receptor for several class 2 cytokines including Interleukins-10, -22, -26, -28A/IFN-lambda 2, -28B/IFN-lambda 3, and -29/IFN-lambda (1, 2). IL-10 R beta associates with ligand-specific receptor subunits to form signaling receptor complexes, e.g. IL-10 R alpha for IL-10 (3, 4), IL-20 R alpha for IL-26 (5, 6), IL-22 R alpha for IL-22 (7, 8), and IL-28 R alpha for IL-28A, IL-28B, and IL-29 (9, 10). IL‑10 R beta is widely expressed, while the associated receptor subunits exhibit differential expression patterns (1). The ligand-specific subunits are responsible for the divergent functions of these cytokines, encompassing immune suppression, promotion or inhibition of inflammation, mucosal defense, antiviral immunity, and hematopoiesis (1). IL-10 R beta deficient mice lack responsiveness to each of those cytokines. IL-10 R beta contributes to ligand binding, but effective signaling is only triggered in the presence of the ligand-specific subunit (8, 9, 11). In the case of IL-10, a cytokine dimer binds to two IL‑10 R alpha /IL-10R1 chains, resulting in recruitment of two IL-10 R beta /IL-10R2 chains (3, 12). Some members of the IL-10 family are monomeric cytokines and interact with single molecules of IL-10 R beta and their ligand‑specific subunit (1). Mature mouse IL-10 R beta consists of a 201 amino acid (aa) extracellular region with two fibronectin type-III domains, a 21 aa transmembrane segment and a 108 aa cytoplasmic domain (13). Within the ECD, mouse IL-10 R beta shares 75% and 87% aa sequence identity with human and rat IL-10 R beta, respectively.
- Commins, S. et al. (2008) J. Allergy Clin. Immunol. 121:1108.
- Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929.
- Kotenko, S.V. et al. (1997) EMBO J. 16:5894.
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- Sheikh, F. et al. (2004) J. Immunol. 172:2006.
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- Xie, M.-H. et al. (2000) J. Biol. Chem. 275:31335.
- Kotenko, S.V. et al. (2003) Nat. Immunol. 4:69.
- Sheppard, P. et al. (2003) Nat. Immunol. 4:63.
- Yoon, S.I. et al. (2006) J. Biol. Chem. 281:35088.
- Pletnev, S. et al. (2005) BMC Struct. Biol. 5:10.
- Gibbs, V.C. and D. Pennica (1997) Gene 183:97.
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