Recombinant Mouse IL-12 R beta 1 Protein, CF
Recombinant Mouse IL-12 R beta 1 Protein, CF Summary
Gln20-Glu561, with a C-terminal 6-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 100 μg/mL in sterile PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Background: IL-12 R beta 1
IL-12 R beta 1 is a 100 kDa type I transmembrane protein that belongs to the gp130/G-CSF R family of cytokine receptors. IL-12 R beta 1 is a common subunit of both the IL-12 and IL-23 receptor complexes which play distinct but related roles in T cell mediated inflammatory reactions (1, 2). Mature mouse IL-12 R beta 1 contains a 546 amino acid (aa) extracellular domain (ECD) with five fibronectin type III repeats, and a 147 aa cytoplasmic domain (3). Within the ECD, mouse IL-12 R beta 1 shares 85% and 52% aa sequence identity with rat and human IL-12 R beta 1, respectively. It shares 16% - 21% aa sequence identity with the ECDs of mouse gp130, LIF R, G-CSF R, and IL-23 R. IL-12 and IL-23 are disulfide linked heterodimeric cytokines that share a common p40 subunit (1, 2). IL-12 R beta 1 interacts with p40 at low affinity but does not transmit signals (3). Increased ligand binding affinity and signaling capacity are gained by association of IL-12 R beta 1 with either IL-12 R beta 2 or IL-23 R (4 - 6). IL-12 R beta 2 and IL-23 R are the signal transducing components of these receptor complexes (4, 7). IL-12 R beta 1 is expressed on activated T cells, NK cells, B cells, macrophages, and microglia (8 - 10). IL-12 induced signaling promotes the development of naïve T cells into IFN-beta producing Th1 cells (11). IL-23 contributes to chronic inflammation by inducing the production of IL-17 by memory T cells (12). Naturally occurring homodimers of p40 can function as antagonists of IL-12 and IL-23 and can also induce macrophage chemotaxis in the absence of IL-12 R beta 2 (13, 14).
- Becker, C. et al. (2005) Inflamm. Bowel Dis. 11:755.
- Hunter, C.A. (2005) Nat. Rev. Immunol. 5:521.
- Chua, A.O. et al. (1995) J. Immunol. 155:4286.
- Parham, C. et al. (2002) J. Immunol. 168:5699.
- Wu, C. et al. (1997) J. Immunol. 159:1658.
- Zou, J. et al. (1997) J. Biol. Chem. 272:6073.
- Presky, D.H. et al. (1996) Proc. Natl. Acad. Sci. 93:14002.
- Wu, C. et al. (1997) Eur. J. Immunol. 27:147.
- Airoldi, I. et al. (2000) J. Immunol. 165:6880.
- Li, J. et al. (2003) J. Neurol. Sci. 215:95.
- Schmitt, E. et al. (1994) Eur. J. Immunol. 24:793.
- Yen, D. et al. (2006) J. Clin. Invest. 116:1310.
- Shimozato, O. et al. (2006) Immunology 117:22.
- Russell, T.D. et al. (2003) J. Immunol. 171:6866.
No product specific FAQs exist for this product, however you mayView all Proteins and Enzyme FAQs
Reviews for Recombinant Mouse IL-12 R beta 1 Protein, CF
There are currently no reviews for this product. Be the first to review Recombinant Mouse IL-12 R beta 1 Protein, CF and earn rewards!
Have you used Recombinant Mouse IL-12 R beta 1 Protein, CF?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image