Recombinant Mouse IL-18 R beta/IL-1 R7 Fc Chimera, CF

R&D Systems | Catalog # 9459-AP

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Key Product Details

  • R&D Systems NS0-derived Recombinant Mouse IL-18 R beta/IL-1 R7 Fc Chimera (9459-AP)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

XP_006495773

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
View all IL-18 R beta/IL-1 R7 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse IL-18 R beta/IL-1 R7 protein
Mouse IL-18 R beta /IL-1 R7
(Glu15 & Phe20-Asp357)
Accession # XP_006495773		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu15, Phe20

Predicted Molecular Mass

66 kDa

SDS-PAGE

74-95 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse IL‑18 R beta /IL‑1 R7 Fc Chimera is coated at 2 μg/mL (100 μL/well), the concentration of Recombinant Mouse IL‑18/IL‑F4 (Catalog # 9139‑IL) that produces 50% optimal binding response is 1-6 μg/mL

Scientific Data Images for Recombinant Mouse IL-18 R beta/IL-1 R7 Fc Chimera, CF

Recombinant Mouse IL-18 R beta/IL-1 R7 Fc Chimera Bioactivity

Recombinant Mouse IL-18 R beta/IL-1 R7 Fc Chimera Bioactivity

When Recombinant Mouse IL-18 R beta /IL-1 R7 Fc Chimera (Catalog # 9459-AP) is coated at 2 µg/mL (100 µL /well), Recombinant Mouse IL-18/IL-1F4 (Catalog # 9139-IL) binds with an ED50 of 1-6 µg/mL.

Formulation, Preparation, and Storage

9459-AP
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-18 R beta/IL-1 R7

IL-18, originally described as an interferon-gamma inducing factor (IGIF), is a member of the IL-1 family of cytokines that has multiple immunoregulatory functions. It has potent IFN-gamma inducing activities and plays a key role in the activation of T helper type 1 (Th1) responses (1). The functional IL-18 receptor complex consists of two components, the IL-18 R alpha (IL-1 R5) and IL-18 R beta (also termed IL-1 R7 and AcPL) subunits. Both subunits are members of the IL-1 receptor superfamily. Although IL-18 R alpha by itself binds IL-18 with low-affinity and IL-18 R beta does not bind IL-18 in vitro, co-expression of IL-18 R alpha and IL-18 R beta is required for high-affinity binding and IL-18 responsiveness (1, 2). Mouse IL-18 R beta cDNA encodes a 614 amino acid (aa) residue precursor type I membrane protein with a 19 aa signal peptide, a 337 aa extracellular region containing three immunoglobulin-like domains, a single transmembrane domain and a 237 aa cytoplasmic domain. Human and mouse IL-18 R beta share 65% aa sequence identity. The expression of IL-18 R beta parallels that of IL-18 R alpha and is detected in numerous tissues including lung, spleen, leukocytes and colon (1).

References

  1. Born, T.L. et al. (1998) J. Biol. Chem. 273:29445.
  2. Wu, C., et al.  (2003) J. Immunol. 273:5571.

Long Name

Interleukin 18 Receptor beta

Alternate Names

AcPL, CD218b, IL-1 R7, IL-18Rb, IL18R beta, IL18RAP

Entrez Gene IDs

8807 (Human); 16174 (Mouse)

Gene Symbol

IL18RAP

UniProt

XP_006495773

Additional IL-18 R beta/IL-1 R7 Products

Product Documents for Recombinant Mouse IL-18 R beta/IL-1 R7 Fc Chimera, CF

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Product Specific Notices for Recombinant Mouse IL-18 R beta/IL-1 R7 Fc Chimera, CF

For research use only

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