Recombinant Mouse IL-20 R beta Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
4388-MR-050

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Recombinant Mouse IL-20 R beta Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to bind recombinant mouse IL-20 in a functional ELISA with an estimated KD<10 nM.
Source
Mouse myeloma cell line, NS0-derived mouse IL-20 R beta protein
Mouse IL-20 R beta
(Asp25-Gly223)
Accession # NP_001028715
KDNIEGRMDP Mouse IgG2A
(Glu98-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Asp14 & Val16
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
49.7 kDa (monomer)
SDS-PAGE
62-66 kDa, reducing conditions

Product Datasheets

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4388-MR

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

4388-MR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: IL-20 R beta

IL-20 receptor beta (IL-20 R beta ), also known as IL-20 R2, is a type I transmembrane glycoprotein in the class II cytokine receptor family. These receptors are characterized by tandem fibronectin type III domains in their extracellular region and the lack of a WSXWS motif (1). Class II cytokine receptors form heterodimeric signaling receptor complexes that mediate class II cytokine signals. Subunits of the different receptor complexes are shared and serve multiple functions (1). Based on the structure of the human receptor, mouse IL-20 R beta should contain a 202 amino acid (aa) extracellular domain (ECD) with two fibronectin type III domains, a 16 aa transmembrane segment, and a 44 aa cytoplasmic domain (2). Within the ECD, mouse IL-20 R beta will share 81% aa sequence identity with human IL-20 R beta. It shares 18%‑29% aa sequence identity with the mouse class II cytokine receptors IFN-alpha R1, IFN-alpha R2, IFN-gamma R1, IFN-gamma R2, IL-10 R alpha, IL-10 R beta, IL-20 R alpha, IL-22BP, IL-22 R alpha, IL-28 R, and tissue factor. IL-20 R beta is generally expressed in psoriatic skin (keratinocytes and select endothelium), rheumatoid arthritis synovial membranes, and hepatocytes of LPS-treated mice, and it contributes to the local inflammatory reaction (3‑6). IL-20 R beta heterodimerizes with IL-20 R alpha to form the receptor complex that mediates IL-19, IL-20 and IL-24 signals (3, 7‑10). It also heterodimerizes with IL-22 R to form the functional receptor complex for IL-20 and IL-24 (7‑9). Binding of these IL-10 family class II cytokines to their receptors induces activation of the JAK-STAT signal transduction pathway.

References
  1. Langer, J.A. et al. (2004) Cytokine Growth Factor Rev. 15:33. 
  2. Accession # NP_001032323 and SwissProt # Q6UXL0.
  3. Blumberg, H. et al. (2001) Cell 104:9. 
  4. Hsu, Y.-H. et al. (2006) Arthritis Rheum. 54:2722. 
  5. Wegenka, U.M. et al. (2007) J. Hepatol. 46:257. 
  6. Chan, J.R. et al. (2006) J. Exp. Med. 203:2577.
  7. Parrish-Novak, J. et al. (2002) J. Biol. Chem. 277:47517.
  8. Dumoutier, L. et al. (2001) J. Immunol. 167:3545.
  9. Wang, M. et al. (2002) J. Biol. Chem. 277:7341.
  10. Pletnev, S. et al. (2003) Biochemistry 42:12617.
Long Name
Interleukin 20 Receptor beta
Entrez Gene IDs
53833 (Human); 213208 (Mouse)
Alternate Names
FNDC6; DIRS1; FNDC6; IL-20 R beta; IL20R beta; IL-20R2; IL20RB interleukin 20 receptor beta; IL20RB; IL-20Rb; MGC34923

Citations for Recombinant Mouse IL-20 R beta Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Interleukin 19 suppresses RANKL-induced osteoclastogenesis via the inhibition of NF-&kappaB and p38MAPK activation and c-Fos expression in RAW264.7 cells
    Authors: M Tsubaki, T Takeda, T Matsuda, Y Yamamoto, A Higashinak, K Yamamoto, K Tsurushima, T Ishizaka, S Nishida
    Cytokine, 2021-05-27;144(0):155591.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Dysregulation of suppressor of cytokine signaling 3 in keratinocytes causes skin inflammation mediated by interleukin-20 receptor-related cytokines.
    Authors: Uto-Konomi A, Miyauchi K, Ozaki N
    PLoS ONE, 2012-07-05;7(7):e40343.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr

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Recombinant Mouse IL-20 R beta Fc Chimera Protein, CF
By Anonymous on 09/28/2020
Application: Immunoassay Standard