Recombinant Mouse IL-22 R alpha 1 Protein, CF

Catalog # Availability Size / Price Qty
4294-MR-050
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Mouse IL-22 R alpha 1 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit IL-22-induced IL-10 secretion by COLO 205 human colorectal adenocarcinoma cells. The ED50 for this effect is 3-10 µg/mL in the presence of 1 ng/mL of rmIL-22.
Source
Mouse myeloma cell line, NS0-derived mouse IL-22 R alpha 1 protein
Thr18-Ala228, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Thr18
Predicted Molecular Mass
24.9 kDa
SDS-PAGE
35-40 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

4294-MR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in sterile PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: IL-22 R alpha 1

The IL-22 receptor, also known as IL-22 R alpha 1 and CRF2-9, is an approximately 65 kDa type I transmembrane glycoprotein that belongs to the type II cytokine receptor family (CRF). IL-22 R alpha 1 contains a 211 amino acid (aa) extracellular domain (ECD) with two fibronectin type III repeats, and a 330 aa cytoplasmic domain (1). Within the ECD, mouse IL-22 R alpha 1 shares 78%, 78%, and 94% aa sequence identity with canine, human, and rat IL-22 R alpha 1, respectively. It shares 20% - 26% aa sequence identity with the ECDs of other class II receptors IL-10 R, IL-20 R, and IL-28 R. IL-22 R alpha 1 associates with either IL-10 R beta or IL-20 R beta to form receptor complexes with distinct ligand selectivities. IL-10 R beta is a shared subunit of the IL-10, -22, -26, -28, and -29 receptors, while IL-20 R beta is a shared subunit of the IL-19, -20, -22, and -24 receptors (2). IL-22 R alpha 1/IL-10 R beta is an IL-22 responsive receptor (3, 4), and IL-22 R alpha 1/IL-20 R beta is an IL-20 or IL-24 responsive receptor (5, 6). In both cases, IL-22 R alpha 1 functions as the high affinity ligand binding subunit, and subsequent association with IL-10 R beta or IL-20 R beta serves to stabilize the complex (3, 6 - 9). IL-22 R alpha 1 contains cytoplasmic motifs for interactions with signal transduction molecules, but association with IL-10 R beta or IL-20 R beta is required for signal transduction (3, 7). IL-22BP functions as a competitive antagonist by binding IL-22 and preventing its association with IL-22 R alpha 1 (8, 10). Even though it is a receptor for interleukins, IL-22 R alpha 1 is not expressed on hematopoietic cells (7, 11, 12). Instead, IL-22 R alpha 1 expression is restricted to epithelial and stromal cells (7, 11 - 14). IL-22 R alpha 1 signaling promotes innate immune responses and wound healing at sites of infection and inflammation. This includes upregulation of antimicrobial, acute phase, proinflammatory, and extracellular matrix proteins as well as proteases (4, 12, 14, 15). IL-22 R alpha 1 signaling also promotes downregulation of proteins involved in keratinocyte differentiation (4, 15).

References
  1. Tachiiri, A. et al. (2003) Genes Immun. 4:153.
  2. Langer, J.A. et al. (2004) Cytokine Growth Factor Rev. 15:33. 
  3. Xie, M.-H. et al. (2000) J. Biol. Chem. 275:31335. 
  4. Boniface, K. et al. (2005) J. Immunol. 174:3695. 
  5. Dumoutier, L. et al. (2001) J. Immunol. 167:3545. 
  6. Wang, M. et al. (2002) J. Biol. Chem. 277:7341. 
  7. Kotenko, S.V. et al. (2001) J. Biol. Chem. 276:2725. 
  8. Li, J. et al. (2004) Int. Immunopharmacol. 4:693.
  9. Logsdon, N.J. et al. (2002) J. Interferon Cytokine Res. 22:1099
  10. Kotenko, S.V. et al. (2001) J. Immunol. 166:7096.
  11. Nagalakshmi, M.L. et al. (2004) Int. Immunopharmacol. 4:577.
  12. Nagalakshmi, M.L. et al. (2004) Int. Immunopharmacol. 4:679.
  13. Aggarwal, S. et al. (2001) J. Interferon Cytokine Res. 21:1047.
  14. Wolk, K. et al. (2004) Immunity 21:241.
  15. Wolk, K. et al. (2006) Eur. J. Immunol. 36:1309.
Long Name
Interleukin 22 Receptor
Entrez Gene IDs
58985 (Human); 230828 (Mouse)
Alternate Names
CRF2-9; CRF2-9interleukin 22 receptor; Cytokine receptor class-II member 9; Cytokine receptor family 2 member 9; IL-22 R alpha 1; IL-22 receptor subunit alpha-1; IL22R alpha 1; IL22R; IL22R1; IL22RA1; IL-22Ra1; IL-22R-alpha-1; IL-TIF-R1; interleukin 22 receptor, alpha 1; interleukin-22 receptor subunit alpha-1; zcytoR11

Citation for Recombinant Mouse IL-22 R alpha 1 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Glycogen synthase kinase-3beta stabilizes the interleukin (IL)-22 receptor from proteasomal degradation in murine lung epithelia.
    Authors: Weathington N, Snavely C, Chen B, Zhao J, Zhao Y, Mallampalli R
    J Biol Chem, 2014;289(25):17610-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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