Recombinant Mouse IL-34 Protein

Formulations:
Catalog # Availability Size / Price Qty
5195-ML-010
Product Details
Citations (9)
FAQs
Reviews (1)

Recombinant Mouse IL-34 Protein Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using M‑NFS‑60 mouse myelogenous leukemia lymphoblast cells. Nakoinz, I. et al. (1990) J. Immunol. 145:860. The ED50 for this effect is 10‑40 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse IL-34 protein
Asn21-Pro235, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Asn21
Structure / Form
Noncovalently-linked homodimer
Predicted Molecular Mass
25.4 kDa
SDS-PAGE
35-40 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

5195-ML

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

5195-ML/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-34

Interleukin 34 (IL-34; also known as uncharacterized protein C16orf77 homolog) is a 39 kDa, secreted cytokine that belongs to no known cytokine family (1). Mouse IL-34 is synthesized as a 235 amino acid (aa) precursor with a 20 aa signal sequence and a 215 aa mature chain (SwissProt # Q8R1R4). The mature chain contains two potential sites for N-linked glycosylation. There are three isoforms for IL-34. Isoform 1 (Q8R1R4-1) is the recombinant mouse IL-34 described in this insert. Isoform 2 (Q8R1R4-2) has an 85 aa substitution for the final 101 aa’s in isoform 1, and isoform 3 (Q8R1R4-3) lacks Q81 in isoform 1. Mouse IL-34 is 71% identical to human IL-34 on the amino acid level. IL-34 is expressed in various tissues, including heart, brain, lung, liver, kidney, spleen, thymus, testes, ovary, small intestine, prostate, and colon, and it is most abundant in the spleen (1). The receptor for IL-34 is colony-stimulating factor 1 receptor (CSF-1R) (1). IL-34 stimulates monocyte viability (1). In functional studies, IL-34, like CSF-1, the other ligand for CSF-1R, stimulated phosphorylation of extracellular signal-regulated kinase-1 and -2 (ERK1/2) in human monocytes (1). In addition, IL-34 promoted the formation of the colony-forming unit-macrophage (CFU-M), a macrophage progenitor, in human bone marrow cultures (1).

References
  1. Lin, H. et al. (2008) Science 320:807.
Long Name
Interleukin 34
Entrez Gene IDs
146433 (Human); 76527 (Mouse)
Alternate Names
C16orf77; IL34; IL-34; interleukin 34

Citations for Recombinant Mouse IL-34 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. A primary neural cell culture model to study neuron, astrocyte, and microglia interactions in neuroinflammation
    Authors: N Goshi, RK Morgan, PJ Lein, E Seker
    J Neuroinflammation, 2020;17(1):155.
    Species: Rat
    Sample Types: Whole Cell
    Applications: Cell Culture
  2. Diverse Requirements for Microglial Survival, Specification, and Function Revealed by Defined-Medium Cultures
    Authors: CJ Bohlen, FC Bennett, AF Tucker, HY Collins, SB Mulinyawe, BA Barres
    Neuron, 2017;94(4):759-773.e8.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Role of chondroitin sulfate (CS) modification in the regulation of protein tyrosine receptor type Z (PTPRZ) activity: pleiotrophin-PTPRZ-A signaling is involved in oligodendrocyte differentiation
    J Biol Chem, 2016;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Interleukin-34 restores blood-brain barrier integrity by upregulating tight junction proteins in endothelial cells.
    Authors: Jin, Shijie, Sonobe, Yoshifum, Kawanokuchi, Jun, Horiuchi, Hiroshi, Cheng, Yi, Wang, Yue, Mizuno, Tetsuya, Takeuchi, Hideyuki, Suzumura, Akio
    PLoS ONE, 2014;9(12):e115981.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  5. CSF-1 receptor-mediated differentiation of a new type of monocytic cell with B cell-stimulating activity: its selective dependence on IL-34.
    Authors: Yamane F, Nishikawa Y, Matsui K, Asakura M, Iwasaki E, Watanabe K, Tanimoto H, Sano H, Fujiwara Y, Stanley E, Kanayama N, Mabbott N, Magari M, Ohmori H
    J Leukoc Biol, 2014;95(1):19-31.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Colony-stimulating factor 1 receptor (CSF1R) signaling in injured neurons facilitates protection and survival.
    Authors: Luo, Jian, Elwood, Fiona, Britschgi, Markus, Villeda, Saul, Zhang, Hui, Ding, Zhaoqing, Zhu, Liyin, Alabsi, Haitham, Getachew, Ruth, Narasimhan, Ramya, Wabl, Rafael, Fainberg, Nina, James, Michelle, Wong, Gordon, Relton, Jane, Gambhir, Sanjiv S, Pollard, Jeffrey, Wyss-Coray, Tony
    J Exp Med, 2013;210(1):157-72.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Bioassay
  7. Cloning and expression of feline colony stimulating factor receptor (CSF-1R) and analysis of the species specificity of stimulation by colony stimulating factor-1 (CSF-1) and interleukin-34 (IL-34).
    Cytokine, 2013;61(2):630-8.
    Species: Feline
    Sample Types: Whole Cells
    Applications: Bioassay
  8. CSF-1 receptor-dependent colon development, homeostasis and inflammatory stress response.
    Authors: Huynh D, Akcora D, Malaterre J, Chan C, Dai X, Bertoncello I, Stanley E, Ramsay R
    PLoS ONE, 2013;8(2):e56951.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  9. The newly discovered cytokine IL-34 is expressed in gingival fibroblasts, shows enhanced expression by pro-inflammatory cytokines, and stimulates osteoclast differentiation.
    Authors: Bostrom E, Lundberg P
    PLoS ONE, 2013;8(12):e81665.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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Recombinant Mouse IL-34 Protein
By Anonymous on 04/17/2020
Application: CellProlif