Recombinant Mouse IL-36 gamma/IL-1F9 (aa 13-164) Protein

Carrier Free

Catalog # Availability Size / Price Qty
6996-IL-010/CF

With Carrier

Catalog # Availability Size / Price Qty
6996-IL-010
Recombinant Mouse IL-36 gamma/IL-1F9 (aa 13-164) Protein Bioactivity
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Product Details
Citations (5)
FAQs
Reviews (1)

Recombinant Mouse IL-36 gamma/IL-1F9 (aa 13-164) Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce IL-6 secretion by NIH‑3T3 mouse embryonic fibroblast cells. Towne, J.E. et al. (2004) J. Biol. Chem. 279:13677. The ED50 for this effect is 3-18 ng/mL.
Source
E. coli-derived mouse IL-36 gamma/IL-1F9 protein
Gly13-Ser164
Accession #
N-terminal Sequence
Analysis
Gly13
Structure / Form
Monomer
Predicted Molecular Mass
17.3 kDa
SDS-PAGE
17 kDa, reducing conditions

Product Datasheets

6996-IL (with carrier)

6996-IL/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

6996-IL

Formulation Lyophilized from a 0.2 μm filtered solution in MES, NaCl, TCEP, EDTA, CHAPS and PEG 8000 with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

6996-IL/CF

Formulation Lyophilized from a 0.2 μm filtered solution in MES, NaCl, TCEP, EDTA, CHAPS and PEG 8000 with Trehalose.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Bioactivity Recombinant Mouse IL-36 gamma/IL-1F9 (aa 13-164) Protein Bioactivity View Larger

Recombinant Mouse IL-36 gamma /IL-1F9 (aa 13-164) (Catalog # 6996-IL) induces IL-6 secretion in the NIH‑3T3 mouse embryonic fibroblast cell line. The ED50 for this effect is 3-18 ng/mL.

SDS-PAGE Recombinant Mouse IL-36 gamma/IL-1F9 (aa 13-164) Protein SDS-PAGE View Larger

1 µg/lane of Recombinant Mouse IL-36 gamma /IL-1F9 (aa 13-164) was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 17 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-36 gamma/IL-1F9

IL-36 gamma [previously called IL-1F9, IL-1 epsilon (epsilon), and IL-1H1] is a member of the IL-1 family which includes IL‑1 beta, IL‑1 alpha, IL‑1ra, IL‑18, IL‑36 Ra (IL‑1F5), IL-36 alpha (IL‑1F6), IL‑36 beta (IL‑1F8), IL‑37 (IL‑1F7) and IL-1F10 (1-5). All family members show a 12  beta -strand, beta ‑trefoil configuration, and are believed to have arisen from a common ancestral gene (2, 3). Mouse IL‑36 gamma is an 18-22 kDa, 164 amino acid (aa) intracellular and secreted protein that contains no signal sequence, no prosegment and no potential N‑linked glycosylation sites (1, 2, 4, 6, 7). Mouse IL‑36 gamma (aa 13-164) shares 58%, 84%, 64% and 60% aa sequence identity with human, rat, equine and bovine IL‑36 gamma, respectively, and 23-57% aa sequence identity with other family members. A 193 aa mouse isoform with a 29 aa N‑terminal extension has been reported (8). Highest levels of IL‑36 gamma are produced by Langerhans cells, keratinocytes, and stomach Chief cells and parietal cells; these cells contribute to first‑line defense against pathogens in the skin, lungs and digestive tract (2, 3, 6, 9). Its expression is induced by LPS treatment of monocytes, and by IL‑ alpha / beta, IL‑17 or TNF‑ alpha treatment of keratinocytes and bronchial epithelia (1, 6, 7, 9-11). Skin IL-36 gamma expression is increased in contact hypersensitivity and psoriasis (1, 6, 11). It is elevated in inflammatory disorders of the lung (such as asthma) and viral infections. Lung IL‑36 gamma and other IL‑36 proteins contribute to neutrophil influx (4, 7, 10). The receptor for IL-36 gamma is a combination of IL‑1 Rrp2, mainly found in epithelia and keratinocytes, and the widely expressed IL‑1 RAcP (4, 7, 9). IL‑36 alpha, beta and gamma all activate NF‑ kappa B and MAPK pathways in an IL‑1 Rrp2 dependent manner, and IL‑36 gamma induces production of inflammatory cytokines and chemokines such as CXCL8/IL‑8 (7, 9, 10). Full-length recombinant IL-36 proteins appear less active than their endogenous counterparts, but trimming of the N-termini enhances their activity (12).

References
  1. Kumar, S. et al. (2000) J. Biol. Chem. 275:10308.
  2. Busfield, S.J. et al. (2000) Genomics 66:213.
  3. Dunn, E. et al. (2001) Trends Immunol. 22:533.
  4. Barksby, H.E. et al. (2007) Clin. Exp. Immunol. 149:217.
  5. Dinarello, C. et al. (2010) Nat. Immunol. 11:973.
  6. Debets, R. et al. (2001) J. Immunol. 167:1440.
  7. Chustz, R.T. et al. (2010) Am. J. Respir. Cell Mol. Biol. 45:145.
  8. NCBI Accession # NP_705731.2.
  9. Towne, J.E. et al. (2004) J. Biol. Chem. 279:13677.
  10. Ramadas, R.A. et al. (2011) Am. J. Respir. Cell Mol. Biol. 44:134.
  11. Johnston, A. et al. (2011) J. Immunol. 186:2613.
  12. Blumberg, H. et al. (2010) J. Immunol. 185:4354.
Long Name
Interleukin 36 gamma/Interleukin 1 Family 9
Entrez Gene IDs
56300 (Human); 215257 (Mouse); 499744 (Rat)
Alternate Names
IL-1 epsilon; IL-1 H1; IL-1 Related Protein 2; IL-1(EPSILON); IL1E; IL-1-epsilon; IL1F9; IL-1F9; IL1H1; IL-1H1; IL-1-Related Protein 2; IL1RP2; IL-1rp2; IL36 gamma; IL-36 gamma; IL36G; interleukin 1 family, member 9; interleukin 1-related protein 2; Interleukin 36, Gamma; Interleukin-1 epsilon; interleukin-1 family member 9; Interleukin-1 homolog 1; Interleukin-36 Gamma

Citations for Recombinant Mouse IL-36 gamma/IL-1F9 (aa 13-164) Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Interleukin-36gamma aggravates macrophage foam cell formation and atherosclerosis progression in ApoE knockout mice
    Authors: M Zhang, J Liu, R Gao, Y Hu, L Lu, C Liu, L Ai, J Pan, L Tian, J Fan
    Cytokine, 2021;146(0):155630.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  2. Opposing Effects of IL-1Ra and IL-36Ra on Innate Immune Response to Pseudomonas aeruginosa Infection in C57BL/6 Mouse Corneas
    Authors: N Gao, R Me, C Dai, B Seyoum, FX Yu
    J. Immunol., 2018;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  3. A cytokine network involving IL-36?, IL-23, and IL-22 promotes antimicrobial defense and recovery from intestinal barrier damage
    Authors: VL Ngo, H Abo, E Maxim, A Harusato, D Geem, O Medina-Con, D Merlin, AT Gewirtz, A Nusrat, TL Denning
    Proc. Natl. Acad. Sci. U.S.A., 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  4. Autocrine and Paracrine Regulation of Keratinocyte Proliferation through a Novel Nrf2-IL-36? Pathway
    J Immunol, 2016;196(11):4663-70.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Interleukin-36gamma is expressed by neutrophils and can activate microglia, but has no role in experimental autoimmune encephalomyelitis.
    Authors: Bozoyan L, Dumas A, Patenaude A, Vallieres L
    J Neuroinflammation, 2015;12(0):173.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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Reviews for Recombinant Mouse IL-36 gamma/IL-1F9 (aa 13-164) Protein

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Recombinant Mouse IL-36 gamma/IL-1F9 (aa 13-164) Protein
By Anonymous on 12/24/2017
Application: Characterization prior to use