Recombinant Mouse IL-Y Protein, CF Summary
Accession # P43432
Accession # Q8K3I6
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 200 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||
Recombinant Mouse IL-Y (Catalog # 9989-IL) inhibits IL‑12 induced IFN-gamma secretion in mouse splenocytes. The ED50 for this effect is 10‑60 ng/mL.
2 μg/lane of Recombinant Mouse IL-Y was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 74‑84 kDa.
Background: IL-Y (IL-12p40/IL-27p28)
IL-Y is a new member of the IL-12 cytokine family consists of IL-12p40 and IL-27p28 subunits (1, 2, 3). The mature mouse IL-12p40 subunit contains 312 amino acids and shares 66% and 93%amino acid (aa) identity with human and rat IL-12p40, respectively. The mature mouse IL-27p28 subunit contains 205 amino acids and shares 70% and 89% aa identity with human and rat IL-27p28, respectively. IL-Y inhibits the differentiation and inflammatory responses of Th1 and Th17 cells while promoting expansion of IL-10 and Foxp3 expressing regulatory T cells (3). Treatment of prediabetic non-obese diabetic mice using adenovirus vector expressing IL-Y prevents the onset of hyperglycemia with reduced expression of inflammatory mediators such as IFN-gamma. IL-Y significantly stimulates a unique cytokine and chemokine expression profile as well as to activate STAT3, in part, through a pathway involving WSX-1 (2).
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