Recombinant Mouse IL-Y Protein, CF

Catalog # Availability Size / Price Qty
9989-IL-050
Recombinant Mouse IL-Y Protein, CF Bioactivity
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Recombinant Mouse IL-Y Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit IL-12 induced IFN-gamma secretion by mouse splenocytes. The ED50 for this effect is 10-60 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse IL-Y (IL-12p40/IL-27p28) protein
Mouse IL-12p40
(Met23-Ser335)
Accession # P43432
GGGSGGGSGGGSMouse IL-27p28
(Phe29-Ser234)
Accession # Q8K3I6
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Met23 (IL-12p40)
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
60 kDa
SDS-PAGE
74-84 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9989-IL

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Data Images

Bioactivity Bioactivity View Larger

Recombinant Mouse IL-Y (Catalog # 9989-IL) inhibits IL‑12 induced IFN-gamma secretion in mouse splenocytes. The ED50 forthis effect is 10‑60 ng/mL.

SDS-PAGE SDS-PAGE View Larger

2 μg/lane of Recombinant Mouse IL-Y was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 74‑84 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-Y (IL-12p40/IL-27p28)

IL-Y is a new member of the IL-12 cytokine family consists of IL-12p40 and IL-27p28 subunits (1, 2, 3). The mature mouse IL-12p40 subunit contains 312 amino acids and shares 66% and 93%amino acid (aa) identity with human and rat IL-12p40, respectively. The mature mouse IL-27p28 subunit contains 205 amino acids and shares 70% and 89% aa identity with human and rat IL-27p28, respectively. IL-Y inhibits the differentiation and inflammatory responses of Th1 and Th17 cells while promoting expansion of IL-10 and Foxp3 expressing regulatory T cells (3). Treatment of prediabetic non-obese diabetic mice using adenovirus vector expressing IL-Y prevents the onset of hyperglycemia with reduced expression of inflammatory mediators such as IFN-gamma. IL-Y significantly stimulates a unique cytokine and chemokine expression profile as well as to activate STAT3, in part, through a pathway involving WSX-1 (2).

References
  1. Hasegawa, H. et al. (2016) Frontiers in Immunology 7:479.
  2. Flores, R. et al. (2015) Eur J Immunol. 45:3114.
  3. Wang, R. et al. (2012) The Journal of Biological Chemistry 287:36012.
Long Name
Interleukin Y
Entrez Gene IDs
246778 (Human)
Alternate Names
IL-Y (IL-12p40/IL-27p28)

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