Recombinant Mouse IL-Y Protein, CF

Catalog # Availability Size / Price Qty
9989-IL-050
Recombinant Mouse IL-Y Protein, CF Bioactivity
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Recombinant Mouse IL-Y Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit IL-12 induced IFN-gamma secretion by mouse splenocytes. The ED50 for this effect is 10-60 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse IL-Y (IL-12p40/IL-27p28) protein
Mouse IL-12p40
(Met23-Ser335)
Accession # P43432
GGGSGGGSGGGSMouse IL-27p28
(Phe29-Ser234)
Accession # Q8K3I6
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Met23 (IL-12p40)
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
60 kDa
SDS-PAGE
74-84 kDa, reducing conditions

Product Datasheets

9989-IL

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Data Images

Bioactivity Recombinant Mouse IL-Y Protein, CF Bioactivity View Larger

Recombinant Mouse IL-Y (Catalog # 9989-IL) inhibits IL-12 induced IFN-gamma secretion in mouse splenocytes. The ED50 forthis effect is 10-60 ng/mL.

SDS-PAGE Recombinant Mouse IL-Y Protein, CF SDS-PAGE View Larger

2 μg/lane of Recombinant Mouse IL-Y was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 74-84 kDa.

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Background: IL-Y (IL-12p40/IL-27p28)

IL-Y is a new member of the IL-12 cytokine family consists of IL-12p40 and IL-27p28 subunits (1, 2, 3). The mature mouse IL-12p40 subunit contains 312 amino acids and shares 66% and 93%amino acid (aa) identity with human and rat IL-12p40, respectively. The mature mouse IL-27p28 subunit contains 205 amino acids and shares 70% and 89% aa identity with human and rat IL-27p28, respectively. IL-Y inhibits the differentiation and inflammatory responses of Th1 and Th17 cells while promoting expansion of IL-10 and Foxp3 expressing regulatory T cells (3). Treatment of prediabetic non-obese diabetic mice using adenovirus vector expressing IL-Y prevents the onset of hyperglycemia with reduced expression of inflammatory mediators such as IFN-gamma. IL-Y significantly stimulates a unique cytokine and chemokine expression profile as well as to activate STAT3, in part, through a pathway involving WSX-1 (2).

References
  1. Hasegawa, H. et al. (2016) Frontiers in Immunology 7:479.
  2. Flores, R. et al. (2015) Eur J Immunol. 45:3114.
  3. Wang, R. et al. (2012) The Journal of Biological Chemistry 287:36012.

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