Recombinant Mouse Integrin alpha 1 beta 1 Protein, CF

R&D Systems | Catalog # 8188-AB

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Key Product Details

  • R&D Systems CHO-derived Recombinant Mouse Integrin alpha 1 beta 1 Protein (8188-AB)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

Q3V3R4(Integrin alpha 1) & P09055 (Integrin beta 1)

Structure / Form

Noncovalently-linked heterodimer

Applications

Bioactivity
View all Integrin alpha 1 beta 1 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived mouse Integrin alpha 1 beta 1 protein
Mouse Integrin alpha 1
(Phe29-Pro1141)
Accession # Q3V3R4		 His-Pro GGGSGGGS Acidic Tail 6-His tag
Mouse Integrin beta 1
(Gln21-Asp728)
Accession # P09055		 His-Pro GGGSGGGS Basic Tail
N-terminus C-terminus

Purity

>95%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Phe29 (Integrin alpha 1) & No results obtained: Gln21 predicted (Integrin beta 1), sequencing might be blocked

Predicted Molecular Mass

132 kDa (Integrin alpha 1) & 86.5 kDa (Integrin beta 1)

SDS-PAGE

120-135 kDa & 180-215 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Collagen I is immobilized at 5 μg/mL, Recombinant Mouse Integrin alpha 1 beta 1 can bind with an apparent Kd <5 nM.
Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation, and Storage

8188-AB
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Integrin alpha 1 beta 1

Integrin alpha 1 beta 1, also called VLA1, is the only alpha 1 integrin family adhesion receptor, one of twelve integrins that share the beta 1 subunit, and one of four collagen-binding integrins (1-6). It is the non-covalent heterodimer of 190-210 kDa alpha 1 (CD49a) and 130 kDa beta 1 (CD29) type I transmembrane glycoprotein subunits. It is found on cells including activated T cells, B cells, monocytes, vascular smooth muscle cells, osteoblasts and adipocytes (2, 7-9). The alpha 1 extracellular domain (ECD) contains an I (inserted) domain which includes the ligand binding site (2, 4). The beta 1 ECD contains a vWFA domain, which participates in binding (3). Each subunit then has a transmembrane sequence and a short cytoplasmic tail. Divalent cations and intracellular (inside-out) signaling convert the dimer from the folded, inactive form to its most active, extended conformation (1, 2). The 1113 amino acid (aa) mouse alpha 1 extracellular domain (ECD) shares 88% and 96% aa sequence identity with human and rat alpha 1, respectively, while the 708 aa mouse beta 1 ECD shares 93% and 98% aa sequence identity with human and rat beta 1, respectively. alpha 1 beta 1 preferentially binds collagens I, IV, VI, XIII and XVI, but also binds laminin (4-11). alpha 1 beta 1 is reported to down-regulate EGF R signaling, increase expression of caveolin-1, reduce production of reactive oxygen species, regulate collagen expression, control MMP collagenase and gelatinase activity, and mediate the renal basement membrane disorder Alport syndrome (11-13). These effects may begin by alpha 1 beta 1 binding of caveolin-1, initiating signaling pathways that involve the phosphatase TC-PTP, kinases ERK and p38, and the transcription factor PPAR-gamma (11-14). alpha 1 beta 1 down-regulates MMP-mediated angiostatin formation, enhancing tumor vascularization (9). alpha 1 beta 1-null mice are deficient in fibroblast collagen IV and laminin-mediated cell spreading and migration, show defects in bone healing, and are resistant to Alport renal fibrosis (10-12, 15). When expressed in the same epithelial cells, alpha 1 beta 1 negatively regulates integrin alpha 2 beta 1-mediated cell adhesion and migration (16).

References

  1. Takada, Y. et al. (2007) Genome Biol. 8:215.
  2. Luo, B-H. et al. (2007) Annu. Rev. Immunol. 25:619.
  3. Argraves, W.S. et al. (1987) J. Cell Biol. 105:1183.
  4. Briesewitz, R. et al. (1993) J. Biol. Chem. 268:2989.
  5. Holers, V.M. et al. (1989) J. Exp. Med. 169:1589.
  6. Tulla, M. et al. (2001) J. Biol. Chem. 276:48206.
  7. Eble, J.A. et al. (2006) J. Biol. Chem. 281:25745.
  8. Hall, D.E. et al. (1990) J. Cell Biol. 110:2175.
  9. Pozzi, A. et al. (2000) Proc. Natl. Acad. Sci. USA 97:2202.
  10. Gardner, H. et al. (1999) J. Cell Sci. 112:263.
  11. Dennis, J. et al. (2010) Am. J. Pathol. 177:2527.
  12. Cosgrove, D. et al. (2008) Am. J. Pathol. 172:761.
  13. Chen, X. et al. (2010) Mol. Cell. Biol. 30:3048.
  14. Borza, C.M. et al. (2010) J. Biol. Chem. 285:40114.
  15. Gardner, H. et al. (1996) Dev. Biol. 175:301.
  16. Abair, T.D. et al. (2008) Exp. Cell Res. 314:3593.

Entrez Gene IDs

3672 (Human)

Gene Symbol

ITGA1

UniProt

Q3V3R4(Integrin alpha 1) & P09055 (Integrin beta 1)

Additional Integrin alpha 1 beta 1 Products

Product Documents for Recombinant Mouse Integrin alpha 1 beta 1 Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse Integrin alpha 1 beta 1 Protein, CF

For research use only

Citations for Recombinant Mouse Integrin alpha 1 beta 1 Protein, CF

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FAQs for Recombinant Mouse Integrin alpha 1 beta 1 Protein, CF

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  • Q: What is the amino acid sequence of the acidic and basic tails?

    A: Acidic and basic tails are added to the protein to help facilitate optimal activity. While we generally include sequence information on the product datasheet, the sequences of these tails are considered confidential information.

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