Recombinant Mouse Integrin alpha 2 beta 1 Protein, CF

Catalog # Availability Size / Price Qty
7828-A2-050
R&D Systems Recombinant Proteins and Enzymes
1 Image
Product Details
FAQs
Supplemental Products
Reviews

Recombinant Mouse Integrin alpha 2 beta 1 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When bovine collagen-II is coated at 10 μg/mL, Recombinant Mouse Integrin alpha 2 beta 1 binds with an apparent KD <1nM.
Source
Chinese Hamster Ovary cell line, CHO-derived mouse Integrin alpha 2 beta 1 protein
Mouse Integrin alpha 2
(Tyr27-Thr1129)
Accession # Q62469.2
His-Pro GGGSGGGS Acidic Tail HHHHHH
Mouse Integrin beta 1
(Gln21-Asp728)
Accession # P09055
His-Pro GGGSGGGS Basic Tail
N-terminus C-terminus
N-terminal Sequence
Analysis
Tyr27 (Integrin  alpha 2) & Gln21 predicted: No results obtained, sequencing might be blocked (Integrin beta 1)
Structure / Form
Noncovalently-linked heterodimer
Predicted Molecular Mass
130 kDa (Integrin  alpha 2) & 86.5 kDa (Integrin beta 1)
SDS-PAGE
120-160 kDa, reducing conditions

Complete Your Experiment

Product Datasheets

You must select a language.

x

7828-A2

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7828-A2

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Integrin alpha 2 beta 1

Integrin alpha 2 beta 1 is one of twelve integrin family adhesion receptors that share the beta 1 subunit (1‑3). It is the non‑covalent heterodimer of 160 kDa alpha 2 (CD49b) and 130 kDa beta 1 (CD29) type I transmembrane glycoprotein subunits. It is one of six very late antigens on activated T cells, designated VLA2 (3). The alpha 2 extracellular domain (ECD) contains an I (inserted) domain which includes the ligand binding site (2, 3). The beta 1 ECD contains a vWFA domain, which participates in binding. Each subunit then has a transmembrane sequence and a short cytoplasmic tail. The dimer is folded when it is least active. Divalent cations and intracellular (inside‑out) signaling convert it to its most active, extended and open conformation (1, 2). The 1103 amino acid (aa) mouse alpha 2 extracellular domain (ECD) shares 93% aa sequence identity with rat and  80‑81% with human, canine, bovine and equine alpha 2, while the 708 aa mouse beta 1 ECD shares 98% aa identity with rat and 93-94% with human, bovine, porcine, ovine, canine and feline beta 1. The I domain‑containing beta 1 integrins ( alpha 1 beta 1, alpha 2 beta 1, alpha 10 beta 1 and alpha 11 beta 1) all bind collagens, with alpha 2 beta 1 preferring collagens I‑III (4, 5). Platelet alpha 2 beta 1, also called GPIa, cooperates with another adhesion protein, GPVI, to coordinate platelet collagen binding and activation (3, 6, 7). Other alpha 2 beta 1 ligands include laminin, decorin, E‑cadherin, and collagen‑like regions of collectin molecules such as C1q (4). Adhesion is synergized by crosstalk with syndecan‑1 or HGF R/c‑Met, and antagonized by crosstalk with integrin alpha 1 beta 1 (8‑10). In addition to expression on selected hematopoietic cells, alpha 2 beta 1 is present on a wide variety of non‑hematopoietic cells (4). Mice deficient in the alpha 2 subunit have defects in innate immune responses, wound mast cell infiltration and angiogenesis, and platelet responses to collagen (6, 11, 12). In innate immunity, alpha 2 beta 1 binding to C1q initiates the complement cascade and co‑stimulates mast cell activation, triggering neutrophil influx (4, 12).

References
  1. Takada, Y. et al. (2007) Genome Biol. 8:215.
  2. Luo, B-H. et al. (2007) Annu. Rev. Immunol. 25:619.
  3. Takada, Y. and M.E. Hemler (1989) J. Cell Biol. 109:397.
  4. Zutter, M.M. and B.T Edelson (2007) Immunobiology 212:343.
  5. McCall-Culbreath, K.D. and M.M. Zutter (2008) Curr. Drug Targets 9:139.
  6. Sarratt, K.L. et al. (2005) Blood 106:1268.
  7. Lecut, C. et al. (2005) Thromb. Haemost. 94:107.
  8. Vuoriluoto, K. et al. (2008) Exp. Cell Res. 314:3369.
  9. McCall-Culbreath, K.D. et al. (2008) Blood 111:3562.
  10. Abair, T.D. et al. (2008) Exp. Cell Res. 314:3593.
  11. Zweers, M. et al. (2006) J. Invest. Dermatol. 127:467.
  12. Edelson, B.T. et al. 2006) Blood 107:143.
Entrez Gene IDs
3673 (Human); 16401 (Mouse)
Alternate Names
Integrin alpha 2 beta 1

FAQs

  1. What is the amino acid sequence of the acidic and basic tails?

    • Acidic and basic tails are added to the protein to help facilitate optimal activity. While we generally include sequence information on the product datasheet, the sequences of these tails are considered confidential information.

View all Proteins and Enzyme FAQs

Reconstitution Buffers

Reviews for Recombinant Mouse Integrin alpha 2 beta 1 Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Mouse Integrin alpha 2 beta 1 Protein, CF and earn rewards!

Have you used Recombinant Mouse Integrin alpha 2 beta 1 Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review