Recombinant Mouse Integrin alpha 4 beta 7 Protein, CF

R&D Systems | Catalog # 8615-A3

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Key Product Details

  • R&D Systems CHO-derived Recombinant Mouse Integrin alpha 4 beta 7 Protein (8615-A3)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

AAH68313 (Integrin alpha 4) & P26011 (Integrin beta 7)

Structure / Form

Noncovalently-linked heterodimer

Applications

Bioactivity
View all Integrin alpha 4 beta 7/LPAM-1 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived mouse Integrin alpha 4 beta 7/LPAM-1 protein
Mouse Integrin alpha 4
Tyr34-Gln970 (Arg591Leu)
Accession # AAH68313		 GS Linker  Acidic Tail HHHHHH
Mouse Integrin beta 7
Glu20-Arg724
Accession # P26011		 GS Linker  Basic Tail
N-terminus C-terminus

Purity

>95%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Tyr34 (Integrin alpha 4), Glu20 (Integrin beta 7)

Predicted Molecular Mass

113 kDa (Integrin alpha 4), 84 kDa (Integrin beta 7)

SDS-PAGE

115 - 170 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of CHO Chinese hamster ovary cells transfected with VCAM-1.
The ED50 for this effect is 0.6-3.6 μg/mL.

Formulation, Preparation, and Storage

8615-A3
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Integrin alpha 4 beta 7/LPAM-1

Integrin alpha 4 beta 7 is an integrin family adhesion receptor that shares subunits with alpha 4 beta 1 (VLA4) and the E-Cadherin receptor, alpha E beta 7 (1). It is a non-covalent heterodimer composed of two type I transmembrane glycoprotein subunits, a 150 kDa alpha 4 (CD49d) subunit and a 130 kDa beta 7 subunit (2, 3). The alpha 4 extracellular domain (ECD) contains an N-terminal beta -propeller structure followed by thigh, calf-1, and calf-2 domains (1). The beta 7 ECD contains a vWFA domain, which interacts with the
alpha 4 beta -propeller to form a binding domain. Metal ion binding sites termed MIDAS and LIMBS promote firm adhesion, and another site termed ADMIDAS is a negative regulatory site that promotes rolling (4-6). The mouse alpha 4 ECD shares 94% and 85% amino acid sequence identity with the rat and human alpha 4 ECD, respectively. The mouse beta 7 ECD shares 94% and 87% amino acid sequence identity with the rat and human beta 7 ECD, respectively. Integrin alpha 4 beta 7 binds the mucosal addressin
MAdCAM-1, as well as VCAM-1 and Fibronectin (7). Integrin alpha 4 beta 7, which is critical for homing to intestinal mucosa, is induced during T cell activation in Peyer’s patches or mesenteric lymph nodes (8, 9). Its expression requires signals from local dendritic and stromal cells, including secreted retinoic acid (10, 11). The HIV-1 envelope protein gp120 binds to the active form of Integrin alpha 4 beta 7, and this may or may not account for the concentration of HIV-1 virus in the gut-associated lymphoid tissue (GALT) (12-14). Integrin alpha 4 beta 7 may also be involved in lymphocyte trafficking in acute intestinal graft vs. host disease (GVHD) (15).

References

  1. Luo, B.H. et al. (2007) Annu. Rev. Immunol. 25:619.
  2. Takada, Y. et al. (1989) EMBO J. 8:1361.
  3. Erle, D.J. et al. (1991) J. Biol. Chem. 266:11009.
  4. Chen, J. et al. (2004) J. Biol. Chem. 279:55556.
  5. Park, E.J. et al. (2007) J. Clin. Invest. 117:2526.
  6. Yu, Y. et al. (2012) J. Cell Biol. 196:131.
  7. Yang, Y. et al. (1998) Eur. J. Immunol. 28:995.
  8. Wagner, N. et al. (1996) Nature 382:366.
  9. Johansson-Lindbom, B. and W.W. Agace (2007) Immunol. Rev. 215:226.
  10. Hammerschmidt, S.I. et al. (2008) J. Exp. Med. 205:2483.
  11. Edele, F. et al. (2008) J. Immunol. 181:3745.
  12. Arthos, J. et al. (2008) Nat. Immunol. 9:301.
  13. Cicala, C. et al. (2009) Proc. Natl. Acad. Sci. USA 106:20877.
  14. Monteiro, P. et al. (2011) J. Immunol. 186:4618.
  15. Chen, Y.B. et al. (2013) Bone Marrow Transplant. 48:598.

Alternate Names

CD49d/beta7, LPAM-1, LPAM1

Entrez Gene IDs

3676 (Human); 16402 (Mouse)

Gene Symbol

ITGA4

UniProt

AAH68313 (Integrin alpha 4) & P26011 (Integrin beta 7)

Additional Integrin alpha 4 beta 7/LPAM-1 Products

Product Documents for Recombinant Mouse Integrin alpha 4 beta 7 Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse Integrin alpha 4 beta 7 Protein, CF

For research use only

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FAQs for Recombinant Mouse Integrin alpha 4 beta 7 Protein, CF

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  • Q: What is the amino acid sequence of the acidic and basic tails?

    A: Acidic and basic tails are added to the protein to help facilitate optimal activity. While we generally include sequence information on the product datasheet, the sequences of these tails are considered confidential information.

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