Recombinant Mouse Integrin alpha 4 beta 7 Protein, CF

Catalog # Availability Size / Price Qty
8615-A3-050
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Recombinant Mouse Integrin alpha 4 beta 7 Protein, CF Summary

Purity
>95%, by SDS-PAGE with silver staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of CHO Chinese hamster ovary cells transfected with VCAM-1. The ED50 for this effect is 0.6-3.6 μg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived mouse Integrin alpha 4 beta 7/LPAM-1 protein
Mouse Integrin alpha 4
Tyr34-Gln970 (Arg591Leu)
Accession # AAH68313
GS Linker  Acidic Tail HHHHHH
Mouse Integrin beta 7
Glu20-Arg724
Accession # P26011
GS Linker  Basic Tail
N-terminus C-terminus
N-terminal Sequence
Analysis
Tyr34 (Integrin alpha 4), Glu20 (Integrin beta 7)
Structure / Form
Noncovalently-linked heterodimer
Predicted Molecular Mass
113 kDa (Integrin alpha 4), 84 kDa (Integrin beta 7)
SDS-PAGE
115 - 170 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8615-A3

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: Integrin alpha 4 beta 7/LPAM-1

Integrin alpha 4 beta 7 is an integrin family adhesion receptor that shares subunits with alpha 4 beta 1 (VLA4) and the E-Cadherin receptor, alpha E beta 7 (1). It is a non-covalent heterodimer composed of two type I transmembrane glycoprotein subunits, a 150 kDa alpha 4 (CD49d) subunit and a 130 kDa beta 7 subunit (2, 3). The alpha 4 extracellular domain (ECD) contains an N-terminal beta -propeller structure followed by thigh, calf-1, and calf-2 domains (1). The beta 7 ECD contains a vWFA domain, which interacts with the
alpha 4 beta -propeller to form a binding domain. Metal ion binding sites termed MIDAS and LIMBS promote firm adhesion, and another site termed ADMIDAS is a negative regulatory site that promotes rolling (4-6). The mouse alpha 4 ECD shares 94% and 85% amino acid sequence identity with the rat and human alpha 4 ECD, respectively. The mouse beta 7 ECD shares 94% and 87% amino acid sequence identity with the rat and human beta 7 ECD, respectively. Integrin alpha 4 beta 7 binds the mucosal addressin
MAdCAM-1, as well as VCAM-1 and Fibronectin (7). Integrin alpha 4 beta 7, which is critical for homing to intestinal mucosa, is induced during T cell activation in Peyer’s patches or mesenteric lymph nodes (8, 9). Its expression requires signals from local dendritic and stromal cells, including secreted retinoic acid (10, 11). The HIV-1 envelope protein gp120 binds to the active form of Integrin alpha 4 beta 7, and this may or may not account for the concentration of HIV-1 virus in the gut-associated lymphoid tissue (GALT) (12-14). Integrin alpha 4 beta 7 may also be involved in lymphocyte trafficking in acute intestinal graft vs. host disease (GVHD) (15).

References
  1. Luo, B.H. et al. (2007) Annu. Rev. Immunol. 25:619.
  2. Takada, Y. et al. (1989) EMBO J. 8:1361.
  3. Erle, D.J. et al. (1991) J. Biol. Chem. 266:11009.
  4. Chen, J. et al. (2004) J. Biol. Chem. 279:55556.
  5. Park, E.J. et al. (2007) J. Clin. Invest. 117:2526.
  6. Yu, Y. et al. (2012) J. Cell Biol. 196:131.
  7. Yang, Y. et al. (1998) Eur. J. Immunol. 28:995.
  8. Wagner, N. et al. (1996) Nature 382:366.
  9. Johansson-Lindbom, B. and W.W. Agace (2007) Immunol. Rev. 215:226.
  10. Hammerschmidt, S.I. et al. (2008) J. Exp. Med. 205:2483.
  11. Edele, F. et al. (2008) J. Immunol. 181:3745.
  12. Arthos, J. et al. (2008) Nat. Immunol. 9:301.
  13. Cicala, C. et al. (2009) Proc. Natl. Acad. Sci. USA 106:20877.
  14. Monteiro, P. et al. (2011) J. Immunol. 186:4618.
  15. Chen, Y.B. et al. (2013) Bone Marrow Transplant. 48:598.
Entrez Gene IDs
3676 (Human); 16402 (Mouse)
Alternate Names
269C wild type; CD49 antigen-like family member D; CD49d antigen; CD49d; CD49d/beta7; CD49Dantigen CD49D, alpha-4 subunit of VLA-4 receptor; IA4; Integrin alpha 4 beta 7; integrin alpha 4; integrin alpha-4 subunit; integrin alpha-4; Integrin alpha-IV; integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor); LPAM1; LPAM-1; MGC90518; very late activation protein 4 receptor, alpha 4 subunit; VLA-4 subunit alpha

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