Recombinant Mouse Integrin alpha L beta 2 Protein, CF Summary
|Mouse Integrin alpha L
Accession # P24063
|His-Pro||GGGSGGGS||Acidic Tail||6-His tag|
|Mouse Integrin beta 2
Accession # P11835
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 400 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Background: Integrin alpha L beta 2
Integrin alpha L beta 2, also called LFA-1, is one of three beta 2 integrin adhesion proteins. The non‑covalent heterodimer of 180 kDa alpha L/CD11a and 95 kDa beta 2/CD18 integrin subunits is expressed on virtually all leukocytes (1‑3). The ligand binding site of LFA-1 is in the N-terminal head region, formed by an interaction of the vWFA
(I, I‑like) domains from each subunit, and the alpha L beta-propeller structure (3‑5). The alpha L subunit contains domains termed thigh, calf-1 and calf-2, while the beta 2 subunit contains a PSI (plexin-semaphorin-integrin) region and four cysteine-rich I-EGF folds (3). Each subunit has a transmembrane sequence and a short cytoplasmic tail connected to the cytoskeleton. Upon activation by “inside‑out” signaling, clustering, or Mg2+ or Mn2+ binding to metal ion-dependent adhesion sites (MIDAS) within the vWFA domains, the molecule unfolds from its inactive, “closed” conformation to expose ligand binding sites (3‑7). Active alpha L beta 2 binds ICAM-1/CD54, ICAM-2, ICAM-3 and JAM-A (1, 8‑10). The adhesion stabilizes interactions between T cells and antigen-presenting cells, decreases the T cell activation threshold, and facilitates transendothelial migration of neutrophils and other leukocytes to sites of inflammation (10‑13). Blockade of LFA-1 may inhibit graft vs. host disease and transplant rejection (14). A constitutively active construct severely impairs immune responses, demonstrating that both activation and de-activation are important (12). Mutations of beta 2, especially in the vWFA domain, cause leukocyte adhesion deficiency (LAD-1) and susceptibility to bacterial infections (15). The 1065 amino acid (aa) mouse alpha L/CD11b ECD shares 87% aa sequence identity with rat, and 70‑74% with human, equine, bovine, porcine, and canine alpha L ECD. Potential mouse alpha L isoforms of 626 and 601 aa have alternate N- or C-termini, respectively. The 679 aa mouse beta 2/CD18 ECD shares 91% aa sequence identity with rat, and 80-82% with human, bovine, canine, and porcine beta 2 ECD.
- Larson, R.S. et al. (1989) J. Cell Biol. 108:703.
- Kishimoto, T.K. et al. (1987) Cell 48:681.
- Hogg, N. et al. (2011) Nat. Rev. Immunol. 11:416.
- Beglova, N. et al. (2002) Nat. Struct. Biol. 9:282.
- Shimaoka, M. et al. (2003) Immunity 19:391.
- Lu, C. et al. (2004) J. Immunol. 173:3972.
- Cairo, C.W. et al. (2006) Immunity 25:297.
- Nortamo, P. et al. (1991) J. Immunol. 146:2530.
- de Fougerolles, A.R. and T.A. Springer (1992) J. Exp. Med. 175:185.
- Ostermann, G. et al. (2002) Nat. Immunol. 3:151.
- Heit, B. et al. (2005) J. Cell Sci. 118:5205.
- Semmrich, M. et al. (2005) J. Exp. Med. 201:1987.
- Dixit, N. et al. (2011) J. Immunol. 187:472.
- Reisman, N.M. et al. (2011) Blood 118:5851.
- Kishimoto, T.K. et al. (1987) Cell 50:193.
Citation for Recombinant Mouse Integrin alpha L beta 2 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Irisin supports integrin-mediated cell adhesion of lymphocytes
Authors: PK Myint, A Ito, MG Appiah, G Obeng, S Darkwah, E Kawamoto, A Gaowa, EJ Park, M Shimaoka
Biochemistry and Biophysics Reports, 2021;26(0):100977.
Sample Types: Whole Cells
Applications: ELISA Capture
What is the amino acid sequence of the acidic and basic tails?
Acidic and basic tails are added to the protein to help facilitate optimal activity. While we generally include sequence information on the product datasheet, the sequences of these tails are considered confidential information.
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