Recombinant Mouse Integrin alpha V beta 1 Protein, CF

Catalog # Availability Size / Price Qty
7705-AV-050
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Mouse Integrin alpha V beta 1 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Mouse Integrin  alpha V beta 1 is coated at 5 μg/mL, Human Fibronectin (Catalog # 1918-FN) binds with an apparent Kd <0.1 nM.
Source
Chinese Hamster Ovary cell line, CHO-derived mouse Integrin alpha V beta 1 protein
Mouse Integrin alpha V
(Phe31-Val988)
Accession # P43406
HP GS Linker Acidic Tail HHHHHH
Mouse Integrin beta 1
(Gln21-Asn728)
Accession # P09055
His  GS Linker Basic Tail
N-terminus C-terminus
N-terminal Sequence
Analysis
Phe31 ( alpha V subunit) & Gln21 predicted ( beta 1 subunit), No results obtained: sequencing might be blocked
Structure / Form
Noncovalently-linked heterodimer
Predicted Molecular Mass
115 kDa ( alpha V subunit) & 86.4 kDa ( beta 1 subunit)
SDS-PAGE
115-165 kDa, reducing conditions

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7705-AV

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7705-AV

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 400 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Integrin alpha V beta 1

Integrin alpha V beta 1 is one of five alpha V and twelve beta 1 containing Integrin family adhesion receptor heterodimers (1‑3). The non‑covalent heterodimer of 170 kDa alpha V and 130 kDa beta 1/CD29 is present on cells that express both subunits, and dimer formation is dependent on the availability of the individual subunits (4). Since the alpha V and beta 1 subunits are widely expressed, the alpha V beta 1 heterodimer potentially forms in many cell types. The 958 aa mouse alpha V extracellular domain (ECD) shares 92‑95% aa sequence identity with human and bovine alpha V, while the 708 aa mouse beta 1 ECD shares 98% aa identity with rat and 93‑94% aa identity with human, bovine, porcine, ovine, canine and feline beta 1. The alpha V ECD contains an N‑terminal beta ‑propeller structure, followed by domains termed thigh, calf‑1 and calf‑2 (1). The beta 1 ECD contains a vWFA domain, which participates in binding. Each subunit then has a transmembrane sequence and a short cytoplasmic tail. The dimer is folded when it is least active. Divalent cations and intracellular (inside‑out) signaling convert it to its most active, extended and open conformation (1). alpha V integrins bind ligands that contain an RGD motif, including vitronectin, fibronectin and osteopontin (4‑9). The relatively weak binding affinity of alpha V beta 1 to vitronectin and fibronectin is thought to facilitate its activity in cyclic binding and release during cell migration (4, 5). In oligodendrocytes, astrocytes and pancreatic beta cells, alpha V beta 1 is expressed early in differentiation when cells are migrating and is down‑regulated when differentiation is complete (5‑7). alpha V beta 1 has also been found to be a receptor for angiopoietin‑2 in Tie2‑deficient glioma cells, and to mediate cell entry of viruses such as foot‑and‑mouth disease virus and human metaneumovirus (10‑12).

References
  1. Hynes, R.O. (2002) Cell 110:673.
  2. Suzuki, S. et al. (1987) J. Biol. Chem. 262:14080.
  3. Argraves, W.S. et al. (1987) J. Cell Biol. 1025:1183.
  4. Koistinen, P. and J. Heino (2002) J. Biol. Chem. 277:24835.
  5. Kaido, T. et al. (2004) J. Biol. Chem. 279:17731.
  6. Milner, R. et al. (1996) J. Neurosci. 16:7240.
  7. Milner, R. et al. (2001) Mol. Cell. Neurosci. 18:108.
  8. Koivisto, L. et al. (2000) Exp. Cell Res. 255:10.
  9. Hu, D.D. et al. (1995) J. Biol. Chem. 270:26232.
  10. Hu, B. et al. (2006) Cancer Res. 66:775.
  11. Jackson, T. et al. (2002) J. Virol. 76:935.
  12. Cseke, G. et al. (2009) Proc. Natl. Acad. Sci. USA 106:1566.
Entrez Gene IDs
3685 (Human)
Alternate Names
CD51; Integrin alpha V beta 1; integrin subunit alpha V; MSK8; VNRA; VTNR

FAQs

  1. What is the amino acid sequence of the acidic and basic tails?

    • Acidic and basic tails are added to the protein to help facilitate optimal activity. While we generally include sequence information on the product datasheet, the sequences of these tails are considered confidential information.

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