Human Fibronectin Protein, CF

Catalog # Availability Size / Price Qty
1918-FN-02M
Product Details
Citations (36)
FAQs
Reviews (5)

Human Fibronectin Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to support cell attachment and spreading when used as a substratum for cell culture. In this application, the recommended concentration for this effect is
1‑5 µg/cm2.

Fibronectin can also be added in the media to support cell spreading at a concentration of 0.5-50 µg/mL.
Optimal concentration depends on cell type as well as the application or research objectives.
Source
Human plasma-derived Fibronectin protein
The human plasma used for the isolation of this product was certified by the supplier to be HIV-1 and HBsAg negative at the time of shipment. Human blood products should always be treated in accordance with universal handling precautions.

Product Datasheets

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1918-FN

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1918-FN

Formulation Supplied as a 0.2 μm filtered solution in Tris-HCl, NaCl and Urea.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, 2 to 8 °C as supplied.
  • 3 months, 2 to 8 °C under sterile conditions after opening.
Avoid vortexing and excessive agitation.
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Background: Fibronectin

Fibronectin (Fn) is a large modular glycoprotein that is found as a polymeric fibrillar network in the extracellular matirx (ECM) and as soluble disulfide-linked dimeric protomers in plasma and other body fluids. The protein subunit is made up of three types of homologous structural repeats termed Fn type I, type II, and type III repeats. Multiple isoforms of the protein formed by alternative splicing at numerous sites, resulting in insertions of extra type III domains (EDA and ECB) or parts of the variable type III connecting segment (V/IIICS), have been identified. Fibronectin is a ligand for fibrin, heparin, chondroitin sulfate, collagen/gelatin, and many integrin receptors. It is involved in multiple cellular processes including cell adhesion/migration, blood clotting, morphogenesis, tissue repair, and cell signaling. Fibronectin functions are mediated by the insoluble polymeric fibrils in the ECM. Conversion of the non-functional soluble Fibronectin to Fibronectin fibrils in the ECM is tightly regulated. The process is initiated by binding of Fibronectin to cell surface integrins, resulting in conformational changes and exposure of cryptic epitopes necessary for polymerization (1, 2).

References
  1. Johansson, S. et al. (1997) Frontiers in Bioscience 2:126.
  2. Geiger, B. et al. (2001) Nature Reviews Molecular Cell Biology 2:793.
Entrez Gene IDs
2335 (Human); 280794 (Bovine)
Alternate Names
CIG; ED-B; fibronectin 1; Fibronectin; FINC; FN; FN1; FNZ; GFND; GFND2; LETS; MSF; SMDCF

Citations for Human Fibronectin Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

36 Citations: Showing 1 - 10
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  1. Neuropilin 1 and its inhibitory ligand mini-tryptophanyl-tRNA synthetase inversely regulate VE-cadherin turnover and vascular permeability
    Authors: N Gioelli, LJ Neilson, N Wei, G Villari, W Chen, B Kuhle, M Ehling, F Maione, S Willox, S Brundu, D Avanzato, G Koulouras, M Mazzone, E Giraudo, XL Yang, D Valdembri, S Zanivan, G Serini
    Nature Communications, 2022;13(1):4188.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. A Step-by-Step Refined Strategy for Highly Efficient Generation of Neural Progenitors and Motor Neurons from Human Pluripotent Stem Cells
    Authors: J Ren, C Li, M Zhang, H Wang, Y Xie, Y Tang
    Cells, 2021;10(11):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. The in vitro multilineage differentiation and maturation of lung and airway cells from human pluripotent stem cell-derived lung progenitors in 3D
    Authors: AL Rodrigues, HY Liu, YW Chen, M Porotto, A Moscona, HW Snoeck
    Nature Protocols, 2021;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Expansion and characterization of bone marrow derived human mesenchymal stromal cells in serum-free conditions
    Authors: S Bhat, P Viswanatha, S Chandanala, SJ Prasanna, RN Seetharam
    Scientific Reports, 2021;11(1):3403.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Adipocytes disrupt the translational programme of acute lymphoblastic leukaemia to favour tumour survival and persistence
    Authors: Q Heydt, C Xintaropou, A Clear, M Austin, I Pislariu, F Miraki-Mou, P Cutillas, K Korfi, M Calaminici, W Cawthorn, K Suchacki, A Nagano, JG Gribben, M Smith, JD Cavenagh, H Oakervee, A Castleton, D Taussig, B Peck, A Wilczynska, L McNaughton, D Bonnet, F Mardakheh, B Patel
    Nature Communications, 2021;12(1):5507.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  6. Artificial Protein Coronas Enable Controlled Interaction with Corneal Epithelial Cells: New Opportunities for Ocular Drug Delivery
    Authors: C Astarita, S Palchetti, M Massaro-Gi, M Di Domenic, F Petrillo, S Boffo, G Caracciolo, A Giordano
    Pharmaceutics, 2021;13(6):.
    Species: N/A
    Sample Types: Bioassay
    Applications: Bioassay
  7. Induction of cyclophilin A by influenza A virus infection facilitates group A Streptococcus coinfection
    Authors: X Bai, W Yang, X Luan, H Li, H Li, D Tian, W Fan, J Li, B Wang, W Liu, L Sun
    Cell Reports, 2021;35(7):109159.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. In vivo monitoring of dynamic interaction between neutrophil and human umbilical cord blood-derived mesenchymal stem cell in mouse liver during sepsis
    Authors: SY Ahn, YS Maeng, YR Kim, YH Choe, HS Hwang, YM Hyun
    Stem Cell Res Ther, 2020;11(1):44.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  9. Targeted gene correction of human hematopoietic stem cells for the treatment of Wiskott -�Aldrich Syndrome
    Authors: R Rai, M Romito, E Rivers, G Turchiano, G Blattner, W Vetharoy, D Ladon, G Andrieux, F Zhang, M Zinicola, D Leon-Rico, G Santilli, AJ Thrasher, A Cavazza
    Nat Commun, 2020;11(1):4034.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Identification of drivers of breast cancer invasion by secretome analysis: insight into CTGF signaling
    Authors: JW Hellinger, F Schömel, JV Buse, C Lenz, G Bauerschmi, G Emons, C Gründker
    Sci Rep, 2020;10(1):17889.
    Species: Human
    Sample Types: Organoid
    Applications: Bioassay
  11. Aerobic and resistance training enhances endothelial progenitor cell function via upregulation of caveolin-1 in mice with type 2 diabetes
    Authors: L Zhai, Y Liu, W Zhao, Q Chen, T Guo, W Wei, Z Luo, Y Huang, C Ma, F Huang, X Dai
    Stem Cell Res Ther, 2020;11(1):10.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Tyroxine Hydroxylase-Positive Neuronal Cell Population is Increased by Temporal Dioxin Exposure at Early Stage of Differentiation from Human Embryonic Stem Cells
    Authors: S Nath Sarma, R Nagano, S Ohsako
    Int J Mol Sci, 2019;20(11):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  13. DLC2 operates as a tumor suppressor gene in breast cancer via the RhoGTPase pathway
    Authors: Z Yang, H Chen, M Shu, Y Zhang, L Xue, Y Lin
    Oncol Lett, 2019;17(2):2107-2116.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  14. Targeting VLA4 integrin and CXCR2 mobilizes serially repopulating hematopoietic stem cells
    Authors: D Karpova, MP Rettig, J Ritchey, D Cancilla, S Christ, L Gehrs, E Chendamara, MO Evbuomwan, M Holt, J Zhang, G Abou-Ezzi, H Celik, E Wiercinska, W Yang, F Gao, LG Eissenberg, RF Heier, SD Arnett, MJ Meyers, MJ Prinsen, DW Griggs, A Trumpp, PG Ruminski, DM Morrow, HB Bonig, DC Link, JF DiPersio
    J. Clin. Invest., 2019;129(7):2745-2759.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  15. Zika virus enhances monocyte adhesion and transmigration favoring viral dissemination to neural cells
    Authors: NV Ayala-Nune, G Follain, F Delalande, A Hirschler, E Partiot, GL Hale, BC Bollweg, J Roels, M Chazal, F Bakoa, M Carocci, S Bourdoulou, O Faklaris, SR Zaki, A Eckly, B Uring-Lamb, F Doussau, S Cianferani, C Carapito, FMJ Jacobs, N Jouvenet, JG Goetz, R Gaudin
    Nat Commun, 2019;10(1):4430.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  16. Kindlin-1 promotes pulmonary breast cancer metastasis
    Authors: S Sarvi, H Patel, J Li, GL Dodd, H Creedon, M Muir, J Ward, JC Dawson, M Lee, J Culley, DM Salter, AH Sims, A Byron, VG Brunton
    Cancer Res., 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  17. Wogonin Suppresses the Activity of Matrix Metalloproteinase-9 and Inhibits Migration and Invasion in Human Hepatocellular Carcinoma
    Authors: M Hong, H Cheng, L Song, W Wang, Q Wang, D Xu, W Xing
    Molecules, 2018;23(2):.
    Applications: Bioassay
  18. Effects of Brain-Derived Neurotrophic Factor on MicroRNA Expression Profile in Human Endothelial Progenitor Cells
    Authors: T He, R Sun, Y Li, ZS Katusic
    Cell Transplant, 2018;0(0):9636897187616.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  19. Zika virus infected primary microglia impairs NPCs proliferation and differentiation
    Authors: J Wang, J Liu, R Zhou, X Ding, Q Zhang, C Zhang, L Li
    Biochem. Biophys. Res. Commun., 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  20. Angiopoietin-4-dependent venous maturation and fluid drainage in the peripheral retina
    Authors: H Elamaa, M Kihlström, E Kapiainen, M Kaakinen, I Miinalaine, S Ragauskas, M Cerrada-Gi, S Mering, M Nätynki, L Eklund
    Elife, 2018;7(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  21. ZEB1-repressed microRNAs inhibit autocrine signaling that promotes vascular mimicry of breast cancer cells
    Authors: EM Langer, ND Kendsersky, CJ Daniel, GM Kuziel, C Pelz, KM Murphy, MR Capecchi, RC Sears
    Oncogene, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  22. Oncogenic miR-19a and miR-19b co-regulate tumor suppressor MTUS1 to promote cell proliferation and migration in lung cancer
    Authors: Y Gu, S Liu, X Zhang, G Chen, H Liang, M Yu, Z Liao, Y Zhou, CY Zhang, T Wang, C Wang, J Zhang, X Chen
    Protein Cell, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  23. Innate CD8??(+) lymphocytes enhance anti-CD40 antibody-mediated colitis in mice
    Authors: AA Kumar, AG Delgado, MB Piazuelo, L Van Kaer, D Olivares-V
    Immun Inflamm Dis, 2017;5(2):109-123.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  24. Senescent peritoneal mesothelium creates a niche for ovarian cancer metastases
    Cell Death Dis, 2016;7(12):e2565.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  25. PPFIA1 drives active ?5?1 integrin recycling and controls fibronectin fibrillogenesis and vascular morphogenesis
    Nat Commun, 2016;7(0):13546.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  26. PPFIA1 drives active ?5?1 integrin recycling and controls fibronectin fibrillogenesis and vascular morphogenesis.
    Authors: Giulia Mana, Fabiana Clapero, Emiliano Panieri, Valentina Panero, Ralph T Böttcher, Hui-Yuan Tseng, Federico Saltarin, Elena Astanina, Katarzyna I Wolanska, Mark R Morgan, Martin J Humphries, Massimo M Santoro, Guido Serini, Donatella Valdembri
    Nature Communications, 2016;0(0):2041-1723.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  27. miR-193a-3p functions as a tumor suppressor in lung cancer by down-regulating ERBB4.
    Authors: Liang H, Liu M, Yan X, Zhou Y, Wang W, Wang X, Fu Z, Wang N, Zhang S, Wang Y, Zen K, Zhang C, Hou D, Li J, Chen X
    J Biol Chem, 2015;290(2):926-40.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  28. CRK proteins selectively regulate T cell migration into inflamed tissues.
    Authors: Huang, Yanping, Clarke, Fiona, Karimi, Mobin, Roy, Nathan H, Williamson, Edward K, Okumura, Mariko, Mochizuki, Kazuhiro, Chen, Emily J, Park, Tae-Ju, Debes, Gudrun F, Zhang, Yi, Curran, Tom, Kambayashi, Taku, Burkhardt, Janis K
    J Clin Invest, 2015;125(3):1019-32.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  29. alpha6beta1- and alphaV-integrins are required for long-term self-renewal of murine embryonic stem cells in the absence of LIF.
    Authors: Cattavarayane S, Palovuori R, Tanjore Ramanathan J, Manninen A
    BMC Cell Biol, 2015;16(0):3.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Adhesion Assay
  30. 3D niche microarrays for systems-level analyses of cell fate.
    Authors: Ranga A, Gobaa S, Okawa Y, Mosiewicz K, Negro A, Lutolf M
    Nat Commun, 2014;5(0):4324.
    Species: N/A
    Sample Types: Protein
    Applications: Bioassay
  31. miR-203 suppresses the proliferation and migration and promotes the apoptosis of lung cancer cells by targeting SRC.
    Authors: Wang, Nan, Liang, Hongwei, Zhou, Yong, Wang, Chen, Zhang, Suyang, Pan, Yi, Wang, Yanbo, Yan, Xin, Zhang, Junfeng, Zhang, Chen-Yu, Zen, Ke, Li, Donghai, Chen, Xi
    PLoS ONE, 2014;9(8):e105570.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  32. Tivantinib (ARQ197) displays cytotoxic activity that is independent of its ability to bind MET.
    Authors: Basilico C, Pennacchietti S, Vigna E, Chiriaco C, Arena S, Bardelli A, Valdembri D, Serini G, Michieli P
    Clin Cancer Res, 2013;19(9):2381-92.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  33. Tumor-stromal crosstalk in invasion of oral squamous cell carcinoma: a pivotal role of CCL7.
    Authors: Jung DW, Che ZM, Kim J, Kim K, Kim KY, Williams D, Kim J
    Int. J. Cancer, 2010;127(2):332-44.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  34. Dithiolethione compounds inhibit Akt signaling in human breast and lung cancer cells by increasing PP2A activity.
    Authors: Switzer CH, Ridnour LA, Cheng RY, Sparatore A, Del Soldato P, Moody TW, Vitek MP, Roberts DD, Wink DA
    Oncogene, 2009;28(43):3837-46.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  35. Inhibition of endothelial progenitor cell differentiation by VEGI.
    Authors: Tian F, Liang PH, Li LY
    Blood, 2009;113(21):5352-60.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  36. Lrp6 is required for convergent extension during Xenopus gastrulation.
    Authors: Tahinci E, Thorne CA, Franklin JL, Salic A, Christian KM, Lee LA, Coffey RJ, Lee E
    Development, 2007;134(22):4095-106.
    Species: Xenopus
    Sample Types: Whole Cells
    Applications: Bioassay

FAQs

  1. What is the difference between Bovine Fibronectin Protein, CF (Catalog # 1030-FN) and Human Fibronectin Protein, CF (Catalog # 1918-FN)?

    • Only the source species for the protein is different. Both carrier-free proteins have been validated to be bioactive with the same assay protocols listed and recommended concentrations fall within the same range, although customers should determine their optimal concentrations for their particular cell type and application. 

  2. What is the molecular weight of Human Fibronectin Protein, CF (Catalog # 1918-FN)?

    • This Human Fibronectin Protein is purified from human plasma. There are multiple isoforms of fibronectin that vary in molecular weight. Due to the varied weight of the different isoforms, the MW of purified fibronectin can run between 180-285 kDa in an SDS-PAGE gel under reducing conditions.

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Reviews for Human Fibronectin Protein, CF

Average Rating: 4.6 (Based on 5 Reviews)

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Human Fibronectin Protein, CF
By Anonymous on 07/28/2022
Application: Cell Adhesion

By Anonymous on 06/15/2022
Application: Binding assay/Protein-protein interaction

Human Fibronectin Protein, CF
By Irving Garcia on 09/29/2020
Application: Cell Adhesion
Reason for Rating: Works great, easy to use.

Human Fibronectin Protein, CF
By Anonymous on 08/04/2019
Application: Binding assay/Protein-protein interaction

Human Fibronectin Protein, CF
By Balaji Mahender on 02/05/2018
Application: Immunoassay Standard