Recombinant Mouse Jagged 2 Fc Chimera Protein, CF

R&D Systems | Catalog # 4748-JG

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Key Product Details

  • R&D Systems NS0-derived Recombinant Mouse Jagged 2 Fc Chimera Protein (4748-JG)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

Q9QYE5

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
View all Jagged 2 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Jagged 2 protein
Mouse Jagged 2
(Met27-Lys1084)
Accession # Q9QYE5		 IEGRMDP Mouse IgG2A
(Glu98-Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met27

Predicted Molecular Mass

140.8 kDa (monomer)

SDS-PAGE

160-165 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to enhance BMP-2 induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. Nobta, M. et al. (2005) J. Biol. Chem. 280:15842.
The ED50 for this effect is 0.3-1.2 µg/mL.

Reviewed Applications

Read 1 review rated 5 using 4748-JG in the following applications:

Formulation, Preparation, and Storage

4748-JG
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and EDTA.
Reconstitution

Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Jagged 2

Mouse Jagged 2 is a 150 kDa (1) member of the Delta-Serrate-Lag-2 (DSL) family of ligands that activate LIN12/Notch proteins, and thereby regulate cell fate determinations during development (2-6). It is a type 1 transmembrane protein that is synthesized as a 1247 amino acid (aa) precursor. It contains a 23 aa signal sequence, a large, 1059 aa extracellular region, a 21 aa transmembrane region, and a short, 144 aa cytoplasmic region. The extracellular region contains four potential N-linked glycosylation sites, a DSL domain, 16 EGF-like repeats, many of which are also sites of calcium binding, and a cysteine-rich region just proximal to the transmembrane segment (3). Mouse Jagged 2 shares 93% and 90% aa sequence identity with rat and human Jagged 2, respectively. Jagged 2 is expressed highest in fetal thymus, epidermis, foregut, dorsal root ganglia, and inner ear (3). In 2-week old mice, the Jagged 2 transcript is most abundant in heart, lung, thymus, skeletal muscle, brain, and testis (3). Functionally, it is suggested that Jagged 2 engages the Notch1 pathway of signal transduction (3). It is involved in the development of the mammalian limb, branchial arches, central and peripheral nervous systems,  gamma delta  T cell lineage differentiation, and the establishment of functional natural killer cell lines (4-6).

References

  1. Tsai, S. et al. (2000) Blood, 96:950.
  2. Shawber, C. et al. (1996) Dev. Biol. 180:370.
  3. Luo, B. et al. (1997) Mol. Cell. Biol. 17:6057.
  4. Valsecchi, V. et al. (1997) Mech. Dev. 69:203.
  5. DeHart, S. et al. (2005) Blood 105:3521.
  6. de La Coste, A. and A.A. Freitas (2006) Immunol. Lett. 102:1.

Alternate Names

HJ2, JAG2

Entrez Gene IDs

3714 (Human); 16450 (Mouse); 29147 (Rat)

Gene Symbol

JAG2

UniProt

Q9QYE5

Additional Jagged 2 Products

Product Documents for Recombinant Mouse Jagged 2 Fc Chimera Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse Jagged 2 Fc Chimera Protein, CF

For research use only

Related Research Areas

Notch Pathway

Citations for Recombinant Mouse Jagged 2 Fc Chimera Protein, CF

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    Application: Cell Proliferation
    Verified Customer | Posted 10/21/2016
    Recombinant Mouse Jagged 2 Fc Chimera Protein, CF 4748-JG

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