Recombinant Mouse KGF/FGF-7 Protein

Formulations:
Catalog # Availability Size / Price Qty
5028-KG-025
Product Details
Citations (9)
FAQs
Reviews

Recombinant Mouse KGF/FGF-7 Protein Summary

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using 4MBr‑5 rhesus monkey epithelial cells. Rubin, J.S. et al. (1989) Proc. Natl. Acad. Sci. USA 86:802. The ED50 for this effect is 10-50 ng/mL.
Source
E. coli-derived mouse KGF/FGF-7 protein
Cys32-Thr194
Accession #
N-terminal Sequence
Analysis
Cys32
Predicted Molecular Mass
18.7 kDa
SDS-PAGE
19 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

5028-KG

Formulation Lyophilized from a 0.2 μm filtered solution in MOPS, Na2SO4, EDTA and DTT with BSA as a carrier protein.
Reconstitution Reconstitute at 250 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

5028-KG/CF

Formulation Lyophilized from a 0.2 μm filtered solution in MOPS, Na2SO4, EDTA and DTT.
Reconstitution Reconstitute at 250 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: KGF/FGF-7

KGF (keratinocyte growth factor), also known as FGF-7 (fibroblast growth factor-7), is one of 22 known members of the mouse FGF family of secreted proteins that plays a key role in development, morphogenesis, angiogenesis, wound healing, and tumorigenesis (1 - 4). KGF expression is restricted to cells of mesenchymal origin. When secreted, it acts as a paracrine growth factor for nearby epithelial cells (1). KGF speeds wound healing by being dramatically upregulated in response to damage to skin or internal structures that results in high local concentrations of inflammatory mediators such as IL-1 and TNF-alpha. (2, 5). KGF promotes cell migration and invasion, and mediates melanocyte transfer to keratinocytes upon UVB radiation (6, 7). It has been used ectopically to avoid chemotherapy-induced oral mucositis in patients with hematological malignancies (1). Deletion of KGF affects kidney development, producing abnormally small ureteric buds and fewer nephrons (8). It also impedes hair follicle differentiation (9). The 194 amino acid (aa) KGF precursor contains a 31 aa signal sequence and, like all other FGFs, an ~120 aa beta -trefoil scaffold that includes receptor- and heparin-binding sites. KGF signals only through the IIIb splice form of the tyrosine kinase receptor, FGF R2 (FGF R2-IIIb/KGF R) (10). Receptor dimerization requires an octameric or larger heparin or heparin sulfate proteoglycan (11). FGF-10, also called KGF2, shares 51% aa identity and similar function to KGF, but shows more limited expression than KGF and uses an additional receptor, FGF R2-IIIc (12). Following receptor engagement, KGF is typically degraded, while FGF-10 is recycled (12). Mature mouse KGF, which is active across species, shares 96% aa sequence identity with human, rat, bovine, equine, and ovine forms.

References
  1. Finch, P.W. and J.S. Rubin (2006) J. Natl. Cancer Inst. 98:812.
  2. Werner, S. et al. (2007) J. Invest. Dermatol. 127:998.
  3. Werner, S. (1998) Cytokine Growth Factor Rev. 9:153.
  4. Mason, I.J. et al. (1994) Mech. Dev. 45:15.
  5. Geer, D.J. et al. (2005) Am. J. Pathol. 167:1575.
  6. Niu, J. et al. (2007) J. Biol. Chem. 282:6001.
  7. Cardinali, G. et al. (2005) J. Invest. Dermatol. 125:1190.
  8. Qiao, J. et al. (1999) Development 126:547.
  9. Guo, L. et al. (1996) Genes Dev. 10:165.
  10. de Georgi, V. et al. (2007) Dermatol. Clin. 25:477.
  11. Hsu, Y-R. et al. (1999) Biochemistry 38:2523.
  12. Belleudi, F. et al. (2007) Traffic 8:1854.
Long Name
Keratinocyte Growth Factor
Entrez Gene IDs
2252 (Human); 403915 (Canine)
Alternate Names
FGF7; FGF-7; fibroblast growth factor 7HBGF-7; HBGF7; HBGF-7; Heparin-binding growth factor 7; keratinocyte growth factor; KGF; KGFfibroblast growth factor 7 (keratinocyte growth factor)

Citations for Recombinant Mouse KGF/FGF-7 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Efficient Differentiation of Mouse Induced Pluripotent Stem Cells into Alveolar Epithelium Type II with a BRD4 Inhibitor
    Authors: T Momozane, E Fukui, S Funaki, M Fujii, Y Kinehara, E Ito, S Miyagawa, Y Ohno, Y Sawa, M Okumura, Y Shintani
    Stem Cells Int, 2019;2019(0):1271682.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. FGF-7 Dictates Osteocyte Cell Processes Through Beta-Catenin Transduction
    Authors: XY Liu, X Li, MR Bai, X Chen, CL Wang, J Xie, L Ye
    Sci Rep, 2018;8(1):14792.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Transcription factor Etv5 is essential for the maintenance of alveolar type II cells
    Authors: Z Zhang, K Newton, SK Kummerfeld, J Webster, DS Kirkpatric, L Phu, J Eastham-An, J Liu, WP Lee, J Wu, H Li, MR Junttila, VM Dixit
    Proc. Natl. Acad. Sci. U.S.A, 2017;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Bioassay
  4. AIM2 Inflammasome Is Critical for Influenza-Induced Lung Injury and Mortality
    Authors: H Zhang, J Luo, JF Alcorn, K Chen, S Fan, J Pilewski, A Liu, W Chen, JK Kolls, J Wang
    J. Immunol., 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Hierarchical patterning modes orchestrate hair follicle morphogenesis
    Authors: JD Glover, KL Wells, F Matthäus, KJ Painter, W Ho, J Riddell, JA Johansson, MJ Ford, CAB Jahoda, V Klika, RL Mort, DJ Headon
    PLoS Biol., 2017;15(7):e2002117.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  6. Image-guided intrathymic injection of multipotent stem cells supports lifelong T-cell immunity and facilitates targeted immunotherapy.
    Authors: Tuckett, Andrea Z, Thornton, Raymond, Shono, Yusuke, Smith, Odette M, Levy, Emily R, Kreines, Fabiana, van den Brink, Marcel R, Zakrzewski, Johannes
    Blood, 2014;123(18):2797-805.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  7. Periostin controls keratinocyte proliferation and differentiation by interacting with the paracrine IL-1alpha/IL-6 loop.
    Authors: Taniguchi K, Arima K, Masuoka M, Ohta S, Shiraishi H, Ontsuka K, Suzuki S, Inamitsu M, Yamamoto K, Simmons O, Toda S, Conway S, Hamasaki Y, Izuhara K
    J Invest Dermatol, 2014;134(5):1295-304.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ELISA (Standard)
  8. LIM kinase regulation of cytoskeletal dynamics is required for salivary gland branching morphogenesis.
    Authors: Ray S, Fanti J, Macedo D, Larsen M
    Mol Biol Cell, 2014;25(16):2393-407.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  9. Self-renewal of embryonic-stem-cell-derived progenitors by organ-matched mesenchyme.
    Authors: Sneddon, Julie B, Borowiak, Malgorza, Melton, Douglas
    Nature, 2012;491(7426):765-8.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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