Klotho, also called Klotho-alpha, is the founding member of the Klotho family within the glycosidase-1 superfamily (1, 2). Klotho is expressed in areas concerned with calcium regulation, predominantly in the kidney distal convoluted tubules, but also in the brain choroid plexus (which produces cerebrospinal fluid) and the parathyroid (1). The 1014 amino acid (aa) type I transmembrane protein contains a 34 aa signal sequence, a 948 aa extracellular domain (ECD) containing two extracellular glycosidase-like domains, a 21 aa transmembrane domain and an 11 aa intracellular domain. Within the ECD, mouse Klotho shares 95%, 87% and 87% aa identity with rat, human and equine Klotho, respectively. Although a truncated 554 aa isoform predicts a soluble 70 kDa form, the soluble form found in plasma and cerebrospinal fluid is a 130 kDa form produced by proteolytic cleavage of the glycosylated 135 kDa full-length Klotho (3, 4). A prominent intracellular 120 kDa form of Klotho is localized to endoplasmic reticulum and Golgi membranes (4). Klotho is named for the Greek goddess who spins the thread of life. The phenotype of Klotho-deficient mice resembles premature aging, including arteriosclerosis, osteoporosis, skin atrophy, infertility, emphysema and premature death (2). Conversely, excess Klotho extends lifespan (5). Klotho acts as a cofactor for interaction of FGF23 with FGF R1 (6). This interaction negatively regulates 1 alpha -hydroxylase, the rate-limiting enzyme in the synthesis of 1,25(OH)2D3 (vitamin D) (7). Klotho-deficient mice show severe hyperphosphatemia and ectopic calcification of soft tissues due to excess vitamin D (2, 7). Both Klotho and Klotho-beta are cofactors for FGF19 binding (8). Klotho also shows glucuronidase activity which activates the renal ion channel TRPV5 to reabsorb urinary calcium (9). Klotho has been reported to downregulate insulin or IGF-1 signaling in adipocytes, to bind and antagonize Wnt molecules, and to facilitate release of parathyroid hormone (10 - 12).
Best Seller
Recombinant Mouse Klotho (aa 35-982) Protein, CF
R&D Systems | Catalog # 1819-KL
Loading...
Key Product Details
- R&D Systems CHO-derived Recombinant Mouse Klotho (aa 35-982) Protein (1819-KL)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
CHO
Accession Number
Applications
Bioactivity
Loading...
Product Specifications
Source
Chinese Hamster Ovary cell line, CHO-derived mouse Klotho protein
Ala35-Lys982, with a C-terminal 6-His tag
Ala35-Lys982, with a C-terminal 6-His tag
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ala35
Predicted Molecular Mass
109.5 kDa
SDS-PAGE
125-130 kDa, reducing conditions
Activity
Measured in a cell proliferation assay using BaF3 mouse pro‑B cells transfected with human FGF RIIIc.
The ED50 for this effect is 0.04-0.4 µg/mL in the presence of 1 µg/mL of Recombinant Human FGF‑23 (Catalog # 2604-FG) and 10 µg/mL of heparin.
The ED50 for this effect is 0.04-0.4 µg/mL in the presence of 1 µg/mL of Recombinant Human FGF‑23 (Catalog # 2604-FG) and 10 µg/mL of heparin.
Reviewed Applications
Read 3 reviews rated 4 using 1819-KL in the following applications:
Formulation, Preparation, and Storage
1819-KL
| Formulation | Supplied as a 0.2 μm filtered solution in PBS, Glycerol and EDTA. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Klotho
References
- Nabeshima, Y. (2006) Sci. Aging Knowl. Environ. 8:pe11.
- Kuro-o, M. et al. (1997) Nature 390:45.
- Shiraki-Iida, T. et al. (1998) FEBS Lett. 424:6.
- Imura, A. et al. (2004) FEBS Lett. 565:143.
- Kurosu, H. et al. (2005) Science 309:1829.
- Kurosu, H. et al. (2006) J. Biol. Chem. 281:6120.
- Tsujikawa, H. et al. (2003) Mol. Endocrinol. 17:2393.
- Wu, X. et al. (2007) J. Biol. Chem. 282:29069.
- Chang, Q. et al. (2005) Science 310:490.
- Yamamoto, M. et al. (2005) J. Biol. Chem. 280:38029.
- Liu, H. et al. (2007) Science 317:803.
- Imura, A. et al. (2007) Science 316:1615.
Alternate Names
KL
Gene Symbol
KL
UniProt
Additional Klotho Products
Product Documents for Recombinant Mouse Klotho (aa 35-982) Protein, CF
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Mouse Klotho (aa 35-982) Protein, CF
For research use only
Related Research Areas
Citations for Recombinant Mouse Klotho (aa 35-982) Protein, CF
Powered by Bioz
Customer Reviews for Recombinant Mouse Klotho (aa 35-982) Protein, CF (3)
4 out of 5
3 Customer Ratings
Have you used Recombinant Mouse Klotho (aa 35-982) Protein, CF?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Customer Images
Showing
1
-
3 of
3 reviews
Showing All
Filter By:
-
Application: In vivo studyVerified Customer | Posted 11/16/2022
-
Application: In vivo studyVerified Customer | Posted 11/02/2022In vivo experimental model as a treatment against kidney injury
-
Application: In vivo studyVerified Customer | Posted 12/12/2018
There are no reviews that match your criteria.
Loading...