Recombinant Mouse L1CAM Fc Chimera Protein, CF

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Recombinant Mouse L1CAM Fc Chimera Protein, CF Summary

Product Specifications

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by the ability of the immobilized protein to support the adhesion of Neuro‑2A mouse neuroblastoma cells. Recombinant Mouse L1CAM Fc Chimera immobilized at 2.5 µg/mL with 100 µL/well will induce  >40% cell adhesion.
Optimal dilutions should be determined by each laboratory for each application.
Mouse myeloma cell line, NS0-derived mouse L1CAM protein
Mouse L1CAM
(Met1-Glu1123) with a Phe176Leu Substitution
Accession # P11627
N-terminus C-terminus
Accession #
N-terminal Sequence
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
150.5 kDa (monomer)
180‑200 kDa, reducing conditions

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in MES and NaCl.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: L1CAM

Neural cell adhesion molecule L1 (NCAM‑L1; also L1‑CAM, L1, and CD171) is a 200 ‑ 220 kDa type I transmembrane glycoprotein of the immunoglobulin superfamily and L1/neurofascin/NgCAM family (1, 2). NCAM-L1 is expressed by neurons, especially by growing axons on their growth cones (2). Non-neuronal cells such as Schwann cells, astrocytes, epithelial cells, and cells of myelomonocytic and lymphoid origin also express NCAM-L1 (2). Full length mouse NCAM-L1 is synthesized as a 1260 amino acid (aa) precursor that contains a 19 aa signal sequence, a 1104 aa extracellular domain (ECD), a 23 aa transmembrane region, and a 114 aa cytoplasmic tail that is highly conserved (3). The ECD contains six Ig-like C2-type domains, five fibronectin type III domains, and 21 potential sites for N‑linked glycosylation. Mouse NCAM-L1 shares 88% aa sequence identity with human NCAM-L1. NCAM-L1 is critical for neural development.  Specifically, NCAM-L1 plays a key role in neuronal cell migration, axon outgrowth, axon fasciculation, synaptogenesis, and myelination (4). NCAM-L1 mediates hemophilic cell-cell interaction but also binds heterophilic ligands like axonin-1, CD24, CD9, neurocan and several integrins (4). It has been shown that NCAM-L1 can undergo membrane-proximal cleavage by ADAM10 and ADAM17, leading to the release of the soluble extracellular domain and the generation of a membrane-bound stub (4). Remaining intact, the soluble extracellular domain has been suggested to serve as a substrate for integrin-mediated cell adhesion, thereby stimulating cellular motility and cell migration (4). It has also been found that NCAM‑L1 plays a role in the ontogeny of human tumors and its expression is linked to poor prognosis (1). Over‑expression promotes tumor-cell invasion and motility, growth in nude mice and tumor metastasis (1). Research shows that proteolytic processing by ADAM10 and presenilin/ gamma -secretase is essential for the nuclear signaling of NCAM-L1 in human carcinoma cell lines (1). Defects in NCAM-L1 are the cause of MASA syndrome (mental retardation, aphasia, shuffling gait and adducted thumbs), which is also known as CRASH syndrome (corpus callosum hypoplasia, psychomotor retardation, adducted thumbs, spastic paraparesis and hydrocephalus (5, 6).

  1. Riedle, S. et al. (2009) Biochem. J. 420:391.
  2. Kenwrick, S. and P. Doherty (1998) Bioessays 20:668.
  3. Crossin, K.L. and L.A. Krushel (2000) Dev. Dyn. 218:260.
  4. Maretzky, T. et al. (2005) Mol. Cell. Biol. 25:9040.
  5. Kamiguchi, H. et al. (1998) Mol. Cell Neurosci. 12:48.
  6. Striha, L. et al. (2000) J. Child Neurol. 15:239.
Long Name
Cell Adhesion Molecule L1
Entrez Gene IDs
3897 (Human); 16728 (Mouse); 50687 (Rat)
Alternate Names
antigen identified by monoclonal R1; CAML1; CAML1N-CAML1; CD171 antigen; CD171; HSAS; HSAS1; L1 cell adhesion molecule; L1CAM; MASA; MIC5; N-CAM-L1; NCAM-L1; neural cell adhesion molecule L1; S10; SPG1

Citations for Recombinant Mouse L1CAM Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Antagonistic L1 Adhesion Molecule Mimetic Compounds Inhibit Glioblastoma Cell Migration In Vitro
    Authors: V Nagaraj, M Mikhail, M Baronio, A Gatto, A Nayak, T Theis, U Cavallaro, M Schachner
    Biomolecules, 2022;12(3):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Neural cell-cell and cell-substrate adhesion through N-cadherin, N-CAM and L1.
    Authors: Wiertz RW, Marani E, Rutten WL
    J Neural Eng, 2011;8(4):046004.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay


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