Recombinant Mouse Laminin alpha 4 Protein, CF Summary
Gln826-Ala1816, with a C-terminal 10-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in Tris and NaCl, pH 6.0.|
|Reconstitution||Reconstitute at 200 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Background: Laminin alpha 4
The alpha 4 chain of laminin (LAMA4) is a secreted ~210 kDa glycoprotein that contributes to the formation of LN411 (laminin-8, subunits alpha 4 beta 1 gamma 1), LN421 (laminin‑9, subunits alpha 4 beta 2 gamma 1), and LN423 (laminin-14, subunits alpha 4 beta 2 gamma 3) (1 ‑ 4). Laminin alpha, beta and gamma subunits interact via alpha -helical domains to create cruciform, disulfide‑linked, coiled-coil laminin heterotrimers (1, 2). LAMA4 is mainly expressed in mesodermal tissues, and is produced by endothelium, cardiac and skeletal muscle fibers, lung mesenchymal cells, pre‑adipocytes, kidney mesangial cells, peripheral nerves and neutrophils (3 ‑ 8). It is found in the subendothelium in muscle, brain, and bone marrow, and in heart, skin, and the perineurium of peripheral nerves (3 ‑ 7, 9, 10). The mouse LAMA4 cDNA encodes 1815 amino acids (aa), including a 24 aa signal sequence and a 1792 aa mature protein that contains three EGF-like repeats (aa 82 ‑ 240) followed by four coiled-coil regions (aa 431 ‑ 799) and five globular, laminin G‑like (LG) domains (aa 826 ‑ 1813). Two potential isoforms exist, one truncated after Ala885 and the second after Ser679. A protease-sensitive region between LG3 and LG4 produces a processed ~180 kDa form that is detected in vivo (10). Within the LG region, mouse LAMA4 shares 91% and 97% aa identity with the equivalent region of human and rat LAMA4, respectively. LAMA4 LG domains serve as Ca++‑mediated receptors for integrins such as alpha v beta 3 and alpha 3 beta 1 (mainly at LG2), fibulin-1 and -2 (multiple LG), and heparan sulfate proteoglycans such as syndecans 2 and 4 (mainly at LG4) (8 ‑ 12). Deletion of LAMA4 in mice results in misalignment of pre- and post-synaptic membranes at neuromuscular junctions, faulty nerve myelination, chronic kidney disease, deficient neutrophil extravasation, and microvessel instability, which causes cardiomyopathy and circulatory dysfunctions (5 ‑ 8, 12 ‑ 14). These results show the importance of LAMA4-containing laminins in the nervous system and the circulation.
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Citations for Recombinant Mouse Laminin alpha 4 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
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Inhibiting multiple forms of cell death optimizes ganglion cells survival after retinal ischemia reperfusion injury
Authors: Q Qin, N Yu, Y Gu, W Ke, Q Zhang, X Liu, K Wang, M Chen
Cell Death & Disease, 2022;13(5):507.
Sample Types: Whole Cells
Laminins affect T cell trafficking and allograft fate.
Authors: Warren K, Iwami D, Harris D, Bromberg J, Burrell B
J Clin Invest, 2014;124(5):2204-18.
Sample Types: Whole Cells
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