Recombinant Mouse LILRC1 Protein, CF

  
  • Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its binding ability in a functional ELISA. When Recombinant Mouse LILRC1 (Catalog # 9288-T4) is coated at 2 µg/mL, Recombinant Human Angiopoietin-like Protein 7/ANGPTL7 (Catalog # 914-AN) binds with an ED50 = 60-360 ng/mL.
  • Source
    Mouse myeloma cell line, NS0-derived Gln17-Asn248, with a C-terminal 6-His tag
  • Accession #
  • N-terminal Sequence
    Analysis
    No results obtained. Gln17 inferred from enzymatic pyroglutamate treatment revealing Glu18
  • Predicted Molecular Mass
    27 kDa
  • SDS-PAGE
    36-50 kDa, reducing conditions
9288-T4
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and NaCl.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
When Recombinant Mouse LILRC1 (Catalog #
9288-T4) is coatedat 2 µg/mL, Recombinant Human Angiopoietin-likeProtein 7/ANGPTL7 (Catalog # 914-AN) binds with an
ED50 = 60-360 ng/mL.
Background: LILRC1
The leukocyte immunoglobulin-like receptors (LILR) comprise a family of activating and inhibitory type immunoreceptors whose genes are located in the same locus that encodes killer cell Ig-like receptors (KIRs) (1). LILRA5, also known as ILT11, LIR-9, CD85f, and LILRC1, consists of a 227 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 10 aa cytoplasmic tail (2, 3). The ECD contains two Ig-like domains (4), and the transmembrane segment contains a positively charged aspartic acid residue which may mediate its association with the signaling molecule, FcR common gamma chain (3, 5). Within the ECD, mouse LILRA5 shares 56% and 85% aa sequence identity with human and rat LILRA5, respectively. LILRA5 is expressed by monocytes, macrophages, and neutrophils (2, 3, 5). It is found as an approximately 40 kDa molecule on the cell surface, while a soluble 25 kDa form can be released into the synovial fluid of rheumatoid arthritis patients (2, 5). Cross-linking of LILRA5 on monocytes induces the expression of pro-inflammatory cytokines (IL-1 beta, IL-6, TNF-alpha) as well as the
anti-inflammatory IL-10 (2, 5). R&D Systems in-house testing indicates that LILRA5 binds to Angiopoietin-like 7, consistent with the demonstrated functional interactions between other members of these protein families (6).
  • References:
    1. Thomas, R. et al. (2010) Clin. Rev. Allergy Immunol. 38:159.
    2. Borges, L. et al. (2003) Blood 101:1484.
    3. Hoelsbrekken, S.E. et al. (2005) Immunogenetics 57:479.
    4. Shiroishi, M. et al. (2006) J. Biol. Chem. 281:19536.
    5. Mitchell, A. et al. (2008) Eur. J. Immunol. 38:3459.
    6. Zheng, J. et al. (2012) Nature 485:656.
  • Long Name:
    Leukocyte Immunoglobulin-like Receptor C1
  • Entrez Gene IDs:
    232801 (Mouse); 691533 (Rat)
  • Alternate Names:
    LILRC1
Related Research Areas
Primary Antibodies
Description Application Cat# Citations Images  

His Tag Antibody

WB, Flow, AP MAB050R  
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His Tag Antibody

WB, Simple Western, Flow, AP MAB050 17  
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His Tag Horseradish Peroxidase-conjugated Antibody

WB, Simple Western MAB050H 3  
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His Tag APC-conjugated Antibody

ICFlow IC050A  
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His Tag PE-conjugated Antibody

ICFlow IC050P 1  
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His Tag Biotinylated Antibody

WB BAM050 1  
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His Tag Alexa Fluor® 488-conjugated Antibody

ICFlow IC050G  
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His Tag PerCP-conjugated Antibody

ICFlow IC050C  
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His Tag Alexa Fluor® 700-conjugated Antibody

ICFlow IC050N  
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Protein Purification Kits
Description Application Cat# Citations Images  

Histidine-tagged Protein Purification Resin

IP999
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