Home / LRRN4 / Recombinant Mouse LRRN4 His-tag Protein, CF

Recombinant Mouse LRRN4 His-tag Protein, CF

Catalog #: 10411-LR Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
10411-LR-050

Save 15% on Select RUO Reagents. See Details. 

Recombinant Mouse LRRN4 His-tag Protein SDS-PAGE
1 Image
Product Details
FAQs
Reviews
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse LRRN4 His-tag Protein, CF Summary

  • R&D Systems NS0-derived Recombinant Mouse LRRN4 His-tag Protein (10411-LR)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Bioassay data are not available.
Source
Mouse myeloma cell line, NS0-derived mouse LRRN4 protein
Gln22-Ser676, with a C-terminal 6-His tag
Accession #
P59383.2
N-terminal Sequence
Analysis
Gln22 inferred from enzymatic pyroglutamate treatment revealing Ser23
Predicted Molecular Mass
72 kDa
SDS-PAGE
110-130 kDa, under reducing conditions

Product Datasheets

You must select a language.

x

10411-LR

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10411-LR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 µg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

SDS-PAGE Recombinant Mouse LRRN4 His-tag Protein SDS-PAGE View Larger

2 μg/lane of Recombinant Mouse LRRN4 His-tag (Catalog # 10411-LR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 110-130 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: LRRN4

LRRN4 (Leucine-rich repeat neuronal protein 4) is a type I transmembrane protein that is a member of the LRRN family. It is expressed in lung, heart, ovary, and neuronal tissues (1-3). Mature mouse LRRN4 is composed of a 657 amino acid (aa) extracellular domain (ECD) that includes ten LRRs, one LRRCT and a fibronectin type-III like domain, a 21 aa transmembrane segment, and a 36 aa cytoplasmic domain. Within the ECD, mouse LRRN4 shares 66% and 84% aa sequence identity with human and rat LRRN4, respectively. LRRN4-deficient mice show defects in the memory retention, suggesting this protein may play an important role in hippocampus-dependent long-lasting memory (1). In addition, LRRN4 is found in about 8% dorsal root ganglion (DRG) neurons (2). These neurons are small-sized neurons that function as nociceptors. LRRN4 expression was decreased in the DRG by sciatic axotomy suggesting that LRRN4 might function as a synaptic adhesion molecule to maintain nociceptive circuits (2). LRRN4 is also expressed in primary mesothelial cells and may be developed as a maker for detection of mesothelioma antigens (4).

References
  1. Bando, T. et al. (2005) Mol. Cell. Biol. 25:4166.
  2. Bando, T. et al. (2012) Neurosci. Lett. 531:24.
  3. Bando, T. et al. (2013) Neurosci. Lett. 548:73.
  4. Kanamori-Katayama, M. (2011) PloS. One. 6(10):e24391.
Long Name
Leucine-rich Repeat Neuronal 4
Entrez Gene IDs
164312 (Human); 320974 (Mouse); 311443 (Rat)
Alternate Names
C20orf75; chromosome 20 open reading frame 75; dJ1056H1.1; FLJ23994; leucine rich repeat neuronal 4; leucine-rich repeat neuronal protein 4; LRRN4; MGC25027; Neuronal leucine-rich repeat protein 4; NLRR4; NLRR-4

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reviews for Recombinant Mouse LRRN4 His-tag Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Mouse LRRN4 His-tag Protein, CF and earn rewards!

Have you used Recombinant Mouse LRRN4 His-tag Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review