Recombinant Mouse Lumican Protein, CF

R&D Systems | Catalog # 2745-LU

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Key Product Details

  • R&D Systems NS0-derived Recombinant Mouse Lumican Protein (2745-LU)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

P51885

Applications

Bioactivity
View all Lumican Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Lumican protein
Gln19-Asn338, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained: Gln19 predicted

Predicted Molecular Mass

37.3 kDa

SDS-PAGE

55-65 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of BCE C/D‑1b bovine corneal endothelial cells.
≥50% of cells will adhere at 10 μg/mL
Optimal dilutions should be determined by each laboratory for each application.

Also measured by its ability to modulate collagen fibrillogenesis.

Reviewed Applications

Read 1 review rated 4 using 2745-LU in the following applications:

Formulation, Preparation, and Storage

2745-LU
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Lumican

Lumican is a 40 kDa member of the family of small leucine-rich repeat proteoglycans (SLRPs) and the class II subfamily (1). Mouse Lumican is synthesized as a 338 amino acid (aa) precursor that contains an 18 aa signal sequence and a 320 aa mature chain (SwissProt #: 51885). The mature chain contains a negatively charged N-terminal domain containing sulfated tyrosine and disulfide bonds, 12 leucine-rich repeats (LRRs), four potential sites of N-linked glycosylation, and a carboxyl terminal domain containing two conserved cysteines (1). Mature mouse Lumican is 98%, 88%, and 70% aa identical to mature rat, human, and chick Lumican, respectively. SLRPs constitute an important fraction of noncollagenous extracellular matrix proteins (ECM) proteins (1, 2). Lumican is expressed in a variety of tissues, including skin, artery, lung, cornea, kidney, bone, aorta, and articular cartilage (1). Lumican’s role in vivo has been found using Lumican null mice. These mice have functional deficits including corneal opacity as well as skin and tendon fragility associated with disorganized and loosely packed collagen fibers (1, 3-6). The abnormal connective tissue phenotype seen in the Lumican null mice shows the importance of the role of Lumican in collagen fibrillogenesis (1). In addition to the control of collagen fibril assembly, Lumican has been shown to play a role in the regulation of cell proliferation (7, 8), migration (8 - 9), and adhesion (9). Lumican’s overexpression has been reported in carcinoid tumor, breast, colorectal, neuroendocrine, uterine cervical and pancreatic cancers (10).

References

  1. Nikitovic, D. et al. (2008) IUBMB Life 60:818.
  2. Blochberger, T.C. et al. (1992) J. Biol. Chem. 267:347.
  3. Chakravarti, S. et al. (1998) J. Cell Biol. 141:1277.
  4. Chakravarti, S. et al. (2000) Invest. Ophthalmol. Vis. Sci. 41:3365.
  5. Jepsen, K.J. et al. (2002) J. Biol. Chem. 277:35532.
  6. Chakravarti, S. et al. (2003) Invest. Ophthalmol. Vis. Sci. 44:2422.
  7. Vuillermoz, B. et al. (2004) Exp. Cell Res. 296:294
  8. Nikitovic, D. et al. (2008) FEBS J. 275:350.
  9. D’Onofrio, M.F. et al. (2008) Biochem. Biophys. Res. Commun. 365:266.
  10. Ishiwata, T. et al. (2007) Oncol. Rep. 18:537.

Alternate Names

LDC, LUM, SLRR2D

Entrez Gene IDs

4060 (Human); 17022 (Mouse)

Gene Symbol

LUM

UniProt

P51885

Additional Lumican Products

Product Documents for Recombinant Mouse Lumican Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Recombinant Mouse Lumican Protein, CF

For research use only

Related Research Areas

Proteoglycans

Citations for Recombinant Mouse Lumican Protein, CF

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    Application: In vitro bioactivity in cell culture
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