Recombinant Mouse MDGA1 Protein, CF

Catalog # Availability Size / Price Qty
7587-MD-050
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Mouse MDGA1 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Able to significantly enhance neurite outgrowth when immobilized at 1.9-15 μg/mL on a nitrocellulose-coated microplate.
Source
Mouse myeloma cell line, NS0-derived mouse MDGA1 protein
Gln19-Pro910, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
No results obtained: Gln19 predicted, sequencing might be blocked
Structure / Form
Noncovalently-linked homodimer
Predicted Molecular Mass
99.8 kDa (monomer)
SDS-PAGE
115-125 kDa, reducing conditions

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7587-MD

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7587-MD

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 300 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: MDGA1

MDGA1 (MAM domain containing glycosylphosphatidylinositol anchor 1) is a 135-140 kDa glycoprotein within the IgCAM superfamily of adhesion molecules (1-3). MDGA1 is thought to mediate neuronal migration, neurite outgrowth, and cell-cell adhesion within the central and peripheral nervous system (1, 4-8). Mouse MDGA1 precursor is a 940 amino acid (aa) protein that produces an 899 aa mature protein with six Ig-like domains, a fibronectin type III domain (aa 622-724), a MAM domain (aa 736-903), and a GPI anchor (aa 917) (1-3). Mature mouse MDGA1 shares 95%, 97%, and 94% aa sequence identity with mature human, rat, and canine MDGA1, respectively. Mouse MDGA1 also shares 52% aa sequence identity with mouse MDGA2, and may also share some functional redundancy (1, 4). MDGA1 is mainly expressed on restricted populations of neurons in the central and peripheral nervous system, such as embryonic neurons destined for cortical layers 2/3, migrating basilar pontine neurons and D1 interneurons of the spinal cord (1, 5-7). Deletion or down‑regulation of mouse MDGA1 slows radial migration of neurons, indicating a role for MDGA1 in radial migration of cortical neurons (4, 5). MAM and Ig-like domains are involved in heterophilic and homophilic adhesion (7, 8). MDGA1 expression is also reported on primary cells and cell lines from leukemias, lymphomas, and tumors of the lung, colon, uterus, stomach and breast (3).

References
  1. Litwack, E.D. et al. (2004) Mol. Cell. Neurosci. 25:263.
  2. Diaz-Lopez, A. et al. (2005) Exp. Cell Res. 307:91.
  3. De Juan, C. et al. (2002) Oncogene 21:3089.
  4. Ishikawa, T. et al. (2011) Dev. Dyn. 240:96.
  5. Takeuchi, A. and D.D.M. O'Leary (2006) J. Neurosci. 26:4460.
  6. Takeuchi, A. et al. (2007) Cereb Cortex. 17:1531.
  7. Fujimura, Y. et al. (2006) Brain Res. 1101:12.
  8. Diaz-Lopez, A. et al. (2011) Cancer Microenviron. 4:23.
Long Name
MAM Domain Containing Glycosylphosphatidylinositol Anchor 1
Entrez Gene IDs
266727 (Human); 74762 (Mouse)
Alternate Names
FLJ45018; GPIM; MAM domain containing glycosylphosphatidylinositol anchor 1; MAMDC3; MDGA1

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