Recombinant Mouse Meteorin Protein

Carrier Free

Catalog # Availability Size / Price Qty
3475-MN-025/CF

With Carrier

Catalog # Availability Size / Price Qty
3475-MN-025
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Mouse Meteorin Protein Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. This protein is able to significantly enhance neurite outgrowth in the range of 60 ng/3 μL drop‑300 ng/3 μL drop.
Source
Mouse myeloma cell line, NS0-derived mouse Meteorin protein
Gly22-Asp291 with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Gly22
Predicted Molecular Mass
30 kDa
SDS-PAGE
33 kDa, reducing conditions

Product Datasheets

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3475-MN (with carrier)

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3475-MN/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3475-MN

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

3475-MN/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Meteorin

Meteorin, named after meteor because this protein can transform glial cells into cells with an elongated tail, is a 30 kDa member of the Meteorin family (1). The secreted protein is also known as glial cell differentiation factor and hypoxia/reoxygenation regulatory factor. Mouse Meteorin is synthesized as a 291 amino acid (aa) precursor with a 21 aa signal sequence and a 270 aa mature chain (SwissProt # Q8C1Q4).  Splicing variants produce two isoforms. Isoform 2 is missing residues 1‑164 found in isomer 1, and has a two aa substitution for aa 165-166. Mouse Meteorin is 81% aa identical to human Meteorin (2). Meteorin is expressed in the central nervous system both during development and in adult mice (2). During development, Meteorin mRNA appears both in the central and peripheral nervous systems with the most prominent levels in neural progenitors, glial progenitors, and cells of the astrocyte lineage. In the adult mouse, Meteorin is detected only in the brain (2). Within the brain, the most prominent expression is found in the cerebellum where it is expressed by glial cells interspersed between Purkinje neurons (2). Meteorin is also detectable in several discrete neuronal populations, such as the superior colliculus, the ocular motor nucleus, the raphe and pontine nuclei, and various thalamic nuclei (2).Functionally, Meteorin plays important roles in both glial cell differentiation and axonal network formation during neurogenesis (1). In addition, research shows that when Meteorin is expressed and secreted by perivascular astrocytes, it up-regulates thrombospondin-1/-2 to attenuate angiogenesis in the surrounding endothelial cells and to promote vascular maturation (3).

References
  1. Nishino, J. et al. (2004) EMBO J. 23:1998.
  2. Jorgensen, J.R. et al. (2009) J. Mol. Neurosci. March 4 Published online.
  3. Park, J.A. et al. (2008) Glia 56:247.
Entrez Gene IDs
79006 (Human); 70083 (Mouse); 287151 (Rat)
Alternate Names
C16orf23; c380A1.2; Meteorin; METRN

Citation for Recombinant Mouse Meteorin Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Meteorin links the bone marrow hypoxic state to hematopoietic stem/progenitor cell mobilization
    Authors: YW Dai, JK Ma, R Jiang, XL Zhan, SY Chen, LL Feng, Q Zhang, TB Liang, K Lv, GJ Yang, JF Lu, J Chen, XJ Lu
    Cell Reports, 2022;40(12):111361.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Bioassay

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