Recombinant Mouse Netrin-1 Protein

Formulations:
Catalog # Availability Size / Price Qty
1109-N1-025
Recombinant Mouse Netrin-1 Protein Data
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Product Details
Citations (27)
FAQs
Reviews (1)

Recombinant Mouse Netrin-1 Protein Summary

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized recombinant rat UNC5H2 Fc Chimera at 5 µg/mL (100 µL/well) can bind Recombinant Mouse Netrin-1 with a linear range of 3‑200 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse Netrin-1 protein
Mouse Netrin-1
(Val22-Ala603)
Accession # AAC52971
IEGR GGGSGGGSGGGS 10-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Val22
Predicted Molecular Mass
68.2 kDa
SDS-PAGE
83 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1109-N1

Formulation Lyophilized from a 0.2 μm filtered solution in PBS and EDTA with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

1109-N1/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS and EDTA with Trehalose.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

SDS-PAGE View Larger

1 μg/lane of Recombinant Mouse Netrin-1 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a band at 83 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Netrin-1

Mouse Netrin-1 is a member of the laminin-related family of axon-guidance molecules, collectively referred to as Netrins (netr is Sanskrit for “one who guides”). The molecule’s cDNA encodes a 603 amino acid (aa) protein precursor that has structural similarity to the N-terminal gamma ‑chain of laminin. It contains a globular domain, three EGF repeats, and a C-terminal heparin-binding domain. Mouse Netrin-1 shares 52% aa identity with mouse Netrin-3, and 98% and 87% aa identity with human and chicken Netrin-1, respectively. Cells reported to express Netrin-1 in the embryo include cells of the floor plate, ventricular zone of the spinal cord, the brain, the ganglionic eminence, and parts of the diencephalon. Netrins were first identified for promoting the outgrowth of commissural axons and are also involved in helping migrating cells and axonal growth cones navigate to their targets. Netrins can provide both attractive and repulsive cues to neurons, depending on the receptors present and cellular context. In the adult, Netrin-1 is likely involved in axon regeneration in peripheral nerves. Netrin-1 has also been shown to be expressed outside of the nervous system and to be involved in development of such tissues as the pancreas, lung, bowel, bone and mammary gland. In non-neural organogenesis, Netrin‑1 provides an adhesive rather than guidance function. The DCC (deleted in colorectal carcinoma), Neogenin, the UNC5 family of receptors, and the adenosine A2b receptors are proposed to be functional receptors for Netrin-1 (1‑7).

References
  1. Puschel, A. (1999) Mech. Dev. 83:65.
  2. Hedgecock, E. and C. Norris (1997) Trends Genet. 13:251.
  3. Kappler, J. et al. (2000) Biochem. Biophys. Res. Commun. 271:287.
  4. Madison, R. et al. (2000) Exp. Neurology 161:563.
  5. Srinivasan, K. et al. (2003) Dev. Cell 4:371.
  6. Livesey, F.J. (1999) Cell Mol. Life Sci. 56:62.
  7. Corset, V. et al. (2000) Nature 407:747.
Entrez Gene IDs
9423 (Human); 18208 (Mouse); 396389 (Chicken)
Alternate Names
netrin 1; Netrin1; Netrin-1; NTN1; NTN1Lnetrin 1, mouse, homolog of

Citations for Recombinant Mouse Netrin-1 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

27 Citations: Showing 1 - 10
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  1. On-Site Ribosome Remodeling by Locally Synthesized Ribosomal Proteins in Axons
    Authors: T Shigeoka, M Koppers, HH Wong, JQ Lin, R Cagnetta, A Dwivedy, J de Freitas, FW van Tartwi, F Ströhl, JM Cioni, J Schaeffer, M Carrington, CF Kaminski, H Jung, WA Harris, CE Holt
    Cell Rep, 2019;29(11):3605-3619.e10.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  2. A Spatiotemporal Sequence of Sensitization to Slits and Semaphorins Orchestrates Commissural Axon Navigation
    Authors: A Pignata, H Ducuing, L Boubakar, T Gardette, K Kindbeiter, M Bozon, S Tauszig-De, J Falk, O Thoumine, V Castellani
    Cell Rep, 2019;29(2):347-362.e5.
    Species: Chicken
    Sample Types: Whole Tissue
    Applications: Bioassay
  3. The m6A reader YTHDF1 regulates axon guidance through translational control of Robo3.1 expression
    Authors: M Zhuang, X Li, J Zhu, J Zhang, F Niu, F Liang, M Chen, D Li, P Han, SJ Ji
    Nucleic Acids Res., 2019;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Netrin-1 attenuates brain injury after middle cerebral artery occlusion via downregulation of astrocyte activation in mice
    Authors: X He, Y Liu, X Lin, F Yuan, D Long, Z Zhang, Y Wang, A Xuan, GY Yang
    J Neuroinflammation, 2018;15(1):268.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  5. Netrin-1 promotes diabetic corneal wound healing through molecular mechanisms mediated via the adenosine 2B receptor
    Authors: Y Zhang, P Chen, G Di, X Qi, Q Zhou, H Gao
    Sci Rep, 2018;8(1):5994.
    Species: Mouse
    Sample Types: In Vivo
  6. Netrin 1 mediates protective effects exerted by insulin-like growth factor 1 on cochlear hair cells
    Authors: K Yamahara, T Nakagawa, J Ito, K Kinoshita, K Omori, N Yamamoto
    Neuropharmacology, 2017;119(0):26-39.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  7. Functional Roles of Netrin-1 in Osteoblast Differentiation
    Authors: T Sato, S Kokabu, Y Enoki, N Hayashi, M Matsumoto, M Nakahira, M Sugasawa, T Yoda
    In Vivo, 2017;31(3):321-328.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  8. A novel role for the DNA repair gene Rad51 in Netrin-1 signalling
    Authors: KA Glendining, D Markie, RJ Gardner, EA Franz, SP Robertson, CL Jasoni
    Sci Rep, 2017;7(0):39823.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  9. An Alzheimer's disease-linked rare mutation potentiates netrin receptor uncoordinated-5C-induced signaling that merges with amyloid ? precursor protein signaling
    Authors: Y Hashimoto, Y Toyama, S Kusakari, M Nawa, M Matsuoka
    J Biol Chem, 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Netrin-1 is a critical autocrine/paracrine factor for osteoclast differentiation.
    Authors: Mediero A, Ramkhelawon B, Perez-Aso M, Moore K, Cronstein B
    J Bone Miner Res, 2015;30(5):837-54.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Regulation of patterned dynamics of local exocytosis in growth cones by netrin-1.
    Authors: Ros O, Cotrufo T, Martinez-Marmol R, Soriano E
    J Neurosci, 2015;35(13):5156-70.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: Bioassay
  12. The Netrin/RGM receptor, Neogenin, controls adult neurogenesis by promoting neuroblast migration and cell cycle exit.
    Authors: O'Leary C, Bradford D, Chen M, White A, Blackmore D, Cooper H
    Stem Cells, 2015;33(2):503-14.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  13. Netrin-1 promotes adipose tissue macrophage retention and insulin resistance in obesity.
    Authors: Ramkhelawon B, Hennessy E, Menager M, Ray T, Sheedy F, Hutchison S, Wanschel A, Oldebeken S, Geoffrion M, Spiro W, Miller G, McPherson R, Rayner K, Moore K
    Nat Med, 2014;20(4):377-84.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  14. The Parkinsonian mimetic, 6-OHDA, impairs axonal transport in dopaminergic axons.
    Authors: Lu, Xi, Kim-Han, Jeong So, Harmon, Steve, Sakiyama-Elbert, Shelly E, O'Malley, Karen L
    Mol Neurodegener, 2014;9(0):17.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  15. Netrin-1 protects against L-Arginine-induced acute pancreatitis in mice.
    Authors: Chen J, Cai Q, Shen P, Yan R, Wang C, Yang D, Fu H, Chen X
    PLoS ONE, 2012;7(9):e46201.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  16. Genetic analysis of DSCAM's role as a Netrin-1 receptor in vertebrates.
    Authors: Palmesino E, Haddick PC, Tessier-Lavigne M, Kania A
    J. Neurosci., 2012;32(2):411-6.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  17. The Parkinsonian mimetic, MPP+, specifically impairs mitochondrial transport in dopamine axons.
    Authors: Kim-Han JS, Antenor-Dorsey JA, O'Malley KL
    J. Neurosci., 2011;31(19):7212-21.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  18. Netrin-1 Regulates Th1/Th2/Th17 Cytokine Production and Inflammation through UNC5B Receptor and Protects Kidney against Ischemia-Reperfusion Injury.
    Authors: Tadagavadi RK, Wang W
    J. Immunol., 2010;185(0):3750-8.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  19. Neural RNA-binding protein Musashi1 controls midline crossing of precerebellar neurons through posttranscriptional regulation of Robo3/Rig-1 expression.
    Authors: Kuwako K, Kakumoto K, Imai T
    Neuron, 2010;67(3):407-21.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  20. Neuronal guidance protein Netrin-1 induces differentiation in human embryonal carcinoma cells.
    Authors: Mancino M, Esposito C, Watanabe K, Nagaoka T, Gonzales M, Bianco C, Normanno N, Salomon DS, Strizzi L
    Cancer Res., 2009;69(5):1717-21.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  21. Hypoxia-inducible factor-dependent induction of netrin-1 dampens inflammation caused by hypoxia.
    Authors: Rosenberger P, Schwab JM, Mirakaj V, Masekowsky E, Mager A, Morote-Garcia JC, Unertl K, Eltzschig HK
    Nat. Immunol., 2009;10(2):195-202.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  22. Netrin-1 attenuates ischemic stroke-induced apoptosis.
    Authors: Wu TW, Li WW, Li H
    Neuroscience, 2008;156(3):475-82.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  23. LIM-only protein 4 interacts directly with the repulsive guidance molecule A receptor Neogenin.
    Authors: Schaffar G, Taniguchi J, Brodbeck T, Meyer AH, Schmidt M, Yamashita T, Mueller BK
    J. Neurochem., 2008;107(2):418-31.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  24. Netrin-1 and kidney injury. I. Netrin-1 protects against ischemia-reperfusion injury of the kidney.
    Authors: Wang W, Reeves WB, Ramesh G
    Am. J. Physiol. Renal Physiol., 2008;294(4):F739-47.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  25. Netrin-1 can affect morphogenesis and differentiation of the mouse mammary gland.
    Authors: Strizzi L, Mancino M, Bianco C, Raafat A, Gonzales M, Booth BW, Watanabe K, Nagaoka T, Mack DL, Howard B, Callahan R, Smith GH, Salomon DS
    J. Cell. Physiol., 2008;216(3):824-34.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  26. Age- and dose-related effects on MSC engraftment levels and anatomical distribution in the central nervous systems of nonhuman primates: identification of novel MSC subpopulations that respond to guidance cues in brain.
    Authors: Isakova IA, Baker K, Dutreil M, Dufour J, Gaupp D, Phinney DG
    Stem Cells, 2007;25(12):3261-70.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Cells
    Applications: Bioassay
  27. Netrin-1 induces angiogenesis via a DCC-dependent ERK1/2-eNOS feed-forward mechanism.
    Authors: Nguyen A, Cai H
    Proc. Natl. Acad. Sci. U.S.A., 2006;103(17):6530-5.
    Species: Bovine
    Sample Types: Whole Cells
    Applications: Bioassay

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Recombinant Mouse Netrin-1 Protein
By Anonymous on 05/12/2016
Application: In vitro bioactivity in cell culture