Recombinant Mouse Nidogen-2 Protein, CF Summary
Optimal dilutions should be determined by each laboratory for each application.
Leu31-Lys1403 with an N-terminal 6-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 100 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Nidogen-2 (also named entactin-2) is a 200 kDa, secreted, monomeric basement membrane glycoprotein (1). Nidogens 1 and 2 are expressed in nearly all basement membranes (1 - 3) where they interact with laminins, collagen type IV and proteoglycan family members to form structural scaffolds (4, 5). Mice deleted for both nidogen genes die at birth due to delayed basement membrane maturation of the lungs and heart (7). However, deletion of one nidogen gives a mild phenotype, indicating that their functions overlap (6, 7). In adult, muscle Nidogen-2 expression is concentrated in the basal lamina of synapses and is required for their maturation and maintenance (8). Both nidogens bind laminin, perlecan and collagens I and IV, but only Nidogen-1 binds fibulins (1, 3). The two nidogens share approximately 40% amino acid (aa) identity in mouse and are structurally similar (1, 4, 6). Cleavage of a 30 aa signal sequence from mouse Nidogen-2 produces a 1373 aa mature protein containing three globular domains (G1 - 3) separated by a link region and an extended rod-shaped segment. The G1 domain is reported to bind type IV collagen, the G2 nidogen ( beta -barrel) domain interacts with perlecan, and the C-terminal G3 beta -propeller structure is associated with laminin binding. The mucin-like link region is longer in Nidogen-2 than Nidogen-1, and contains both N- and O-glycosylation (2, 8). It contains one EGF-like motif, and an RGD motif that binds alpha 3 beta 1 integrins. The rod-shaped segment contains four additional EGF-like motifs, two of which bind calcium, and two thyroglobulin type 1 domains that serve as a binding site for alpha v beta 3 integrins. If species‑specific insertions and deletions are disregarded, mature mouse Nidogen-2 shares 80%, 92%, 74%, 73%, 71% and 68% aa identity with human, rat, bovine, porcine, canine and equine Nidogen-2, respectively.
- Kohfeldt, K. et al. (1998) J. Mol. Biol. 282:99.
- Miosge, N. et al. (2001) Histochem. J. 33:523.
- Salmivirta, K. et al. (2002) Exp. Cell Res. 279:188.
- Hohenester, E. and J. Engel (2002) Matrix Biol. 21:115.
- Charonis, A. et al. (2005) Curr. Med. Chem. 12:1495.
- Schymeinsky, J. et al. (2002) Mol. Cell. Biol. 22:6820.
- Bader, B.L. et al. (2005) Mol. Cell. Biol. 25:6846.
- Fox, M.A. et al. (2008) Neural Dev. 3:24.
Citation for Recombinant Mouse Nidogen-2 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
The growth factor-like adipokine tartrate-resistant acid phosphatase 5a interacts with the rod G3 domain of adipocyte-produced nidogen-2.
Authors: Patlaka C, Mai H, Lang P, Andersson G
Biochem Biophys Res Commun, 2014;454(3):446-452.
Sample Types: N/A
Applications: Western Blot
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