Recombinant Mouse NPRA/NPR1 Protein, CF

R&D Systems | Catalog # 9726-NR

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Key Product Details

  • R&D Systems NS0-derived Recombinant Mouse NPRA/NPR1 Protein (9726-NR)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

P18293
View all NPRA/NPR1 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse NPRA/NPR1 protein
Ser29-Glu469, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser29

Predicted Molecular Mass

50 kDa

SDS-PAGE

59-71 kDa, reducing conditions

Activity

Bioassay data are not available.

Scientific Data Images for Recombinant Mouse NPRA/NPR1 Protein, CF

The Innovator Series.

The Innovator Series.

R&D Systems proteins are almost always sold with a bioassay to indicate activity. However, we recognize that sometimes proteins might be novel, and their bioactivity may not be well understood. In addition, some researchers may wish to use polypeptides to make antibodies. To facilitate the advancement of new science, we now offer our Innovator Series of proteins.

Formulation, Preparation, and Storage

9726-NR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NPRA/NPR1

Mouse Natriuretic Peptide Receptor-1 (NPR1), also known as NPRA, ANP-R1 or guanlyl cyclase-A, is a 130 kDa member of the guanylyl cyclase family of proteins. NPR1 is a type I transmembrane glycoprotein that contains a 441 amino acid extracellular domain (ECD) (aa 29‑469) for ligand binding, and a 567 amino acid cytoplasmic region that contains both a protein kinase domain and a guanylate cyclase domain. NPR1 is expressed most highly in kidney, adrenal and adipose tissue (1). NPR1 operates as an oligomer and binds both ANP (atrial natriuretic peptide) and BNP (B-type natriuretic peptide). Binding of ANP to the extracellular ligand binding domain, plus ATP to the intracellular kinase domain activates a cytoplasmic guanylate cyclase (1). This process induces bronchodilation and a reduction in blood pressure (2). Over the extracellular domain, mouse NPR1 is 97% and 86% identical to rat and human NPR1, respectively.

References

  1. Koller K.J. et al. (1991) Science 252:120.
  2. Oliver, P.M. et al. (1998). PNAS 95:2547.

Long Name

Natriuretic Peptide Receptor A

Alternate Names

ANPa, ANPRA, GC-A, GUCY2A, NPR1, NPRA, Pndr

Entrez Gene IDs

4881 (Human); 18160 (Mouse); 24603 (Rat)

Gene Symbol

NPR1

UniProt

P18293

Additional NPRA/NPR1 Products

Product Documents for Recombinant Mouse NPRA/NPR1 Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse NPRA/NPR1 Protein, CF

For research use only

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