Recombinant Mouse NPRA/NPR1 Protein, CF

Catalog # Availability Size / Price Qty
9726-NR-050
The Innovator Series.
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Recombinant Mouse NPRA/NPR1 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Bioassay data are not available.
Source
Mouse myeloma cell line, NS0-derived mouse NPRA/NPR1 protein
Ser29-Glu469, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Ser29
Predicted Molecular Mass
50 kDa
SDS-PAGE
59-71 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9726-NR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity not tested The Innovator Series. View Larger

R&D Systems proteins are almost always sold with a bioassay to indicate activity. However, we recognize that sometimes proteins might be novel, and their bioactivity may not be well understood. In addition, some researchers may wish to use polypeptides to make antibodies. To facilitate the advancement of new science, we now offer our Innovator Series of proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NPRA/NPR1

Mouse Natriuretic Peptide Receptor-1 (NPR1), also known as NPRA, ANP-R1 or guanlyl cyclase-A, is a 130 kDa member of the guanylyl cyclase family of proteins. NPR1 is a type I transmembrane glycoprotein that contains a 441 amino acid extracellular domain (ECD) (aa 29‑469) for ligand binding, and a 567 amino acid cytoplasmic region that contains both a protein kinase domain and a guanylate cyclase domain. NPR1 is expressed most highly in kidney, adrenal and adipose tissue (1). NPR1 operates as an oligomer and binds both ANP (atrial natriuretic peptide) and BNP (B-type natriuretic peptide). Binding of ANP to the extracellular ligand binding domain, plus ATP to the intracellular kinase domain activates a cytoplasmic guanylate cyclase (1). This process induces bronchodilation and a reduction in blood pressure (2). Over the extracellular domain, mouse NPR1 is 97% and 86% identical to rat and human NPR1, respectively.

References
  1. Koller K.J. et al. (1991) Science 252:120.
  2. Oliver, P.M. et al. (1998). PNAS 95:2547.
Long Name
Natriuretic Peptide Receptor A
Entrez Gene IDs
4881 (Human); 18160 (Mouse); 24603 (Rat)
Alternate Names
ANPa; ANP-A; ANPRA; ANPR-A; ANPRAEC 4.6.1.2; atrial natriuretic peptide receptor 1; Atrial natriuretic peptide receptor type A; atrionatriuretic peptide receptor A; GC-A; Guanylate cyclase A; GUC2A; GUCY2A; natriuretic peptide A type receptor; natriuretic peptide receptor A; natriuretic peptide receptor A/guanylate cyclase A (atrionatriuretic peptidereceptor A); NPR1; NPRA; NPR-A; Pndr

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