Recombinant Mouse OCAM/NCAM2 His-tag Protein, CF Summary
Met1-Asn697, with a C-terminal 6-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 500 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Recombinant Mouse OCAM/NCAM2 His-tag (Catalog # 10154-CM) supports the adhesion of T47D human breast cancer cells. The ED50 for this effect is 0.6-3.6 μg/mL.
2 μg/lane of Recombinant Mouse OCAM/NCAM2 His-tag (Catalog # 10154-CM) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 95-110 kDa.
OCAM (olfactory cell adhesion molecule), also known as NCAM2 (neural cell adhesion molecule 2) and RNCAM (Rb‑8 neural cell adhesion molecule) is an approximately 100 kDa type I transmembrane glycoprotein belonging to the NCAM family within the immunoglobulin superfamily (1‑5). OCAM dimerizes and participates in homophilic adhesion, but unlike NCAM1/CD56, it is not polysialic acid‑glycosylated, and does not participate in heparin sulfate binding or heterophilic adhesion (2). The 837 amino acid (aa) form of human or mouse OCAM contains a 19 aa signal sequence, a 678 aa extracellular domain (ECD) with five Ig‑like C2‑type domains and two fibronectin type III domains, a transmembrane domain and a 119 aa cytoplasmic domain (2‑4). The first two Ig‑like domains mediate homodimer formation in trans (6). In mouse, a glycosyl phosphatidylinositol‑linked form may show differential expression and function on olfactory axons (1‑3, 7). The ECD of mouse OCAM shares 93%, 98% and 92% aa sequence identity with human, rat, and bovine OCAM, respectively. OCAM is expressed by a subset of axons in the olfactory, vomeronasal, and retrosplenial cortex in a zone‑specific manner (1‑3, 7‑9). It is thought to be important for organization of axons and dendrites and segregation of axodendritic and dendrodendritic synapses within glomeruli (1, 7, 10, 11). Because OCAM is encoded on chromosome 21 in humans, it is implicated in neurological abnormalities in Down Syndrome (trisomy 21) (1, 3, 4). It may also be implicated in development of autism and Alzheimer's disease, and over‑expressed in some breast and prostate cancers (1, 5).
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- Kulahin, N. et al. (2011) Structure 19:203.
- Alenius, M. and S. Bohm (2003) Development 130:917.
- von Campenhausen, H. et al. (1997) Neuroreport 8:2607.
- Ichinohe, N. et al. (2003) Eur. J. Neurosci. 18:1764.
- Walz, A. et al. (2006) Mol. Cell. Neurosci. 32:1.
- Borisovska, M. et al. (2011) J. Physiol. 589:1927.
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