Recombinant Mouse OCAM/NCAM2 His-tag Protein, CF

Catalog # Availability Size / Price Qty
10154-CM-050
Recombinant Mouse OCAM/NCAM2 His-tag Protein, CF Bioactivity
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Recombinant Mouse OCAM/NCAM2 His-tag Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of T47D human breast cancer cells. The ED50 for this effect is 0.6-3.6 μg/mL.
Source
Mouse myeloma cell line, NS0-derived mouse OCAM/NCAM2 protein
Met1-Asn697, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Leu20
Predicted Molecular Mass
76.8 kDa
SDS-PAGE
95-110 kDa, under reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10154-CM

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Bioactivity Bioactivity View Larger

Immobilized Recombinant Mouse OCAM/NCAM2 His-tag (Catalog # 10154-CM)supports the adhesion of  T47D humanbreast cancer cells. The ED50 for this effect is 0.6-3.6 μg/mL.

SDS-PAGE SDS-PAGE View Larger

2 μg/lane of Recombinant Mouse OCAM/NCAM2 His-tag(Catalog # 10154-CM) was resolved with SDS-PAGE under reducing (R) andnon-reducing (NR) conditions and visualized by Coomassie®Blue staining, showing bands at 95-110 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: OCAM/NCAM2

OCAM (olfactory cell adhesion molecule), also known as NCAM2 (neural cell adhesion molecule 2) and RNCAM (Rb‑8 neural cell adhesion molecule) is an approximately 100 kDa type I transmembrane glycoprotein belonging to the NCAM family within the immunoglobulin superfamily (1‑5). OCAM dimerizes and participates in homophilic adhesion, but unlike NCAM1/CD56, it is not polysialic acid‑glycosylated, and does not participate in heparin sulfate binding or heterophilic adhesion (2). The 837 amino acid (aa) form of human or mouse OCAM contains a 19 aa signal sequence, a 678 aa extracellular domain (ECD) with five Ig‑like C2‑type domains and two fibronectin type III domains, a transmembrane domain and a 119 aa cytoplasmic domain (2‑4). The first two Ig‑like domains mediate homodimer formation in trans (6). In mouse, a glycosyl phosphatidylinositol‑linked form may show differential expression and function on olfactory axons (1‑3, 7). The ECD of mouse OCAM shares 93%, 98% and 92% aa sequence identity with human, rat, and bovine OCAM, respectively. OCAM is expressed by a subset of axons in the olfactory, vomeronasal, and retrosplenial cortex in a zone‑specific manner (1‑3, 7‑9). It is thought to be important for organization of axons and dendrites and segregation of axodendritic and dendrodendritic synapses within glomeruli (1, 7, 10, 11). Because OCAM is encoded on chromosome 21 in humans, it is implicated in neurological abnormalities in Down Syndrome (trisomy 21) (1, 3, 4). It may also be implicated in development of autism and Alzheimer's disease, and over‑expressed in some breast and prostate cancers (1, 5).

References
  1. Winther, M. et al. (2012) Int. J. Biochem. Cell Biol. 44:441.
  2. Yoshihara, Y. et al. (1997) J. Neurosci. 17:5930.
  3. Alenius, M. and S. Bohm (1997) J. Biol. Chem. 272:26083.
  4. Paoloni-Giacobino, A. et al. (1997) Genomics 43:43.
  5. Takahashi, S. et al. (2011) Cancer Sci. 102:808.
  6. Kulahin, N. et al. (2011) Structure 19:203.
  7. Alenius, M. and S. Bohm (2003) Development 130:917.
  8. von Campenhausen, H. et al. (1997) Neuroreport 8:2607.
  9. Ichinohe, N. et al. (2003) Eur. J. Neurosci. 18:1764.
  10. Walz, A. et al. (2006) Mol. Cell. Neurosci. 32:1.
  11. Borisovska, M. et al. (2011) J. Physiol. 589:1927.
Long Name
Opioid-binding and Cell Adhesion Molecule/Neural Cell Adhesion Molecule 2
Entrez Gene IDs
4685 (Human); 17968 (Mouse)
Alternate Names
NCAM2; N-CAM-2; NCAM-2; NCAM21MGC51008; neural cell adhesion molecule 2 precursor10; neural cell adhesion molecule 2; OCAM; Rb-8; RNCAM

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