Recombinant Mouse OLT-2/TARM1 Fc Chimera Protein, CF Summary
Accession # B6A8R8
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 500 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Recombinant Mouse OLT-2/TARM1 Fc Chimera (10000-TA) inhibits IFN-gammasecretion by human peripheral blood mononuclear cells in the presence ofanti-CD3 antibody. The ED50 for this effect is 1-10 μg/mL.
2 μg/lane of Recombinant Mouse OLT-2/TARM1 Fc Chimera was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 70-80 kDa and 140-160 kDa, respectively.
TARM-1 (T cell interacting, activating receptor on myeloid cells-1), also name OLT-2 (OSCAR-like Transcript 2), is a type 1 membrane protein expressed by neutrophils, inflammatory monocytes, macrophages, and dendritic cells. Mature mouse TARM-1 contains a 242 amino acid (aa) long extracellular domain with two Ig-like C2 domains, a 21 aa long transmembrane domain and a 9 aa long cytoplasmic domain. The extracellular domain of mouse TARM-1 shares 81.6% and 46.5% aa identity with rat and human TARM-1 respectively. TARM-1 associates with the ITAM bearing adaptor FcR gamma, but not with DAP10 or DAP12. TARM1 expression is also up-regulated by bone marrow-derived macrophages and dendritic cells following stimulation with TLR agonists in vitro. TARM1 receptor stimulation on macrophages and neutrophils co-stimulate the secretion of proinflammatory cytokines induced by TLR ligands, such as LPS. TARM1 Fc fusion protein inhibits anti-CD3 induced CD4+ cell activation, suggesting that TARM1 ectodomain interacts with an unidentified receptor on T cells to inhibit T cell activation (1).
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