Recombinant Mouse OSCAR Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
1633-OS-050
Recombinant Mouse OSCAR Fc Chimera Protein, CF Binding Activity
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Recombinant Mouse OSCAR Fc Chimera Protein, CF Summary

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Bovine Collagen I is coated at 10 µg/mL, Recombinant Mouse OSCAR Fc Chimera binds with an ED50 of 80-640 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse OSCAR protein
Mouse OSCAR
(Asp19-Asn228)
Accession # Q8VBT3
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Asp19
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
49 kDa
SDS-PAGE
56-66 kDa, reducing conditions

Product Datasheets

1633-OS

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Data Image

Binding Activity Recombinant Mouse OSCAR Fc Chimera Protein, CF Binding Activity View Larger

When Bovine College I is coated at 10 µg/mL, Recombinant MouseOSCAR Fc Chimera binds with an ED50 of 80‑640 ng/mL.

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Background: OSCAR

OSCAR (Osteoclast-associated immunoglobulin-like receptor), is a type I transmembrane protein of the leukocyte receptor complex (LRC) family (1). The mature mouse OSCAR includes a 213 amino acid (aa) extracellular domain (ECD) with two Ig domains homology to PIR family proteins, a 17 aa transmembrane region, and a 17 aa cytoplasmic domain. The ECD of mouse OSCAR shares 77% and 84% aa sequence identity with the ECD of human and rat OSCAR, respectively (2). OSCAR is specifically expressed by pre-osteoclasts and signals via FcR gamma (3). OSCAR co-stimulates one of the major FcR gamma‑associated pathways required for osteoclastogenesis in vivo (3). Collagen type I and collagen type II serve as ligands for OSCAR (4). OSCAR binds to specific motifs within fibrillary collagens in the extracellular matrix (ECM) (3). OSCAR promoted osteoclastogenesis in vivo, and its binding to the collagen motif led to signaling that increased numbers of osteoclasts in culture (3). OSCAR contains two Ig domains, D1 and D2. Direct binding assays on OSCAR molecules showed that D2, but not D1, is critical for collagen binding (5). OSCAR may contribute to the pathogenesis and severity of a number of diseases including osteoporosis, atherosclerosis, chronic obstructive pulmonary disease and rheumatoid arthritis (5).

References
  1. Kim, N. et al. (2002) J. Exp. Med. 195:201.
  2. Haywood, J. et al. (2016) Proc. Natl. Acad. Sci. USA 113:1038.
  3. Barrow, A.D. et al. (2011) J. Clin. Invest. 121:3505.
  4. Shultz, H.S. et al. (2016) Eur. J. Immunol. 46:952.
  5. Zhou, L. et al. (2016) Blood 127:529.
Long Name
Osteoclast-associated Receptor
Entrez Gene IDs
126014 (Human); 232790 (Mouse)
Alternate Names
hOSCAR; MGC33613; OSCAR; osteoclast associated receptor OSCAR-S1; osteoclast associated receptor OSCAR-S2; osteoclast associated, immunoglobulin-like receptor; osteoclast-associated immunoglobulin-like receptor; Osteoclast-associated receptor; PIGR3; PIgR-3; Poly-Ig receptor 3; Polymeric immunoglobulin receptor 3

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